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    Nucleic acid construct for expressing active substances which can be activated by proteases, and preparation and use
    3.
    发明授权
    Nucleic acid construct for expressing active substances which can be activated by proteases, and preparation and use 失效
    用于表达可由蛋白酶活化的活性物质的核酸构建体，以及制备和使用

    公开(公告)号：US06670147B1

    公开(公告)日：2003-12-30

    申请号：US09256237

    申请日：1999-02-24

    申请人： Hans Heinrich Heidtmann ,  Rolf Mueller ,  Hans-Harald Sedlacek

    发明人： Hans Heinrich Heidtmann ,  Rolf Mueller ,  Hans-Harald Sedlacek

    IPC分类号： C12P2106

    CPC分类号： C12N9/6432 ,  A61K48/00 ,  C12N15/63 ,  C12N15/85 ,  C12N2830/007 ,  C12N2830/008 ,  C12N2840/20 ,  C12Y304/21006

    摘要： The invention relates to a nucleic acid construct for expressing an active substance which is activated by an enzyme which is released from mammalian cells, which construct comprises the following components: a) at least one promoter element, b) at least one DNA sequence which encodes an active compound (protein B), c) a least one DNA sequence which encodes an amino acid sequence (part structure C) which can be cleaved specifically by an enzyme which is released from a mammalian cell, and d) at least one DNA sequence which encodes a peptide or protein (part structure D) which is bound to the active compound (protein B) by way of the cleavable amino acid sequence (part structure C) and inhibits the activity of the active compound (protein B), and also to the use of the nucleic acid construct for preparing a drug for treating diseases.

    摘要翻译： 本发明涉及用于表达由哺乳动物细胞释放的酶活化的活性物质的核酸构建物，该构建体包含以下组分：a）至少一个启动子元件，b）至少一个编码 活性化合物（蛋白B），c）至少一个DNA序列，其编码可以被从哺乳动物细胞释放的酶特异性切割的氨基酸序列（部分结构C），和d）至少一个DNA序列 其编码通过可切割氨基酸序列（部分结构C）与活性化合物（蛋白B）结合并抑制活性化合物（蛋白B）的活性的肽或蛋白质（部分结构D），并且还 涉及使用核酸构建体来制备用于治疗疾病的药物。

    Oncogene- or virus-controlled expression systems
    5.
    发明授权
    Oncogene- or virus-controlled expression systems 失效
    致癌基因或病毒控制的表达系统

    公开(公告)号：US06465246B1

    公开(公告)日：2002-10-15

    申请号：US09196099

    申请日：1998-11-20

    申请人： Rolf Mueller ,  Hans-Harald Sedlacek

    发明人： Rolf Mueller ,  Hans-Harald Sedlacek

    IPC分类号： C12N1509

    CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K48/00 ,  C12N15/635 ,  C12N15/85 ,  C12N2830/007 ,  C12N2840/20

    摘要： Nucleic acid constructs for expressing an effector gene, with the nucleic acid construct comprising a promoter I (component a) which controls the expression of a transcription factor gene (component b), a transcription factor gene (component b), a promoter II (component c) to which the gene product of the transcription factor gene binds and which controls the expression of an effector gene (component d), and effector gene (component d), wherein the activity of the gene product of the transcription factor gene depends on one or more cellular regulatory proteins which bind to this gene product and affect its activity, and isolated cells containing the nucleic acid constructs, can be used for preparing a drug for treating diseases and in methods of treating diseases.

    摘要翻译： 用于表达效应基因的核酸构建体，其核酸构建体包含控制转录因子基因（组分b）表达的启动子I（组分a），转录因子基因（组分b），启动子II（组分 c）转录因子基因的基因产物结合并控制效应基因（组分d）和效应基因（组分d）的表达，其中转录因子基因的基因产物的活性取决于一个 或更多结合该基因产物并影响其活性的细胞调节蛋白和含有核酸构建体的分离的细胞可用于制备用于治疗疾病的药物和治疗疾病的方法。

    Self-enhancing, pharmacologically controllable expression systems
    6.
    发明授权
    Self-enhancing, pharmacologically controllable expression systems 失效

    公开(公告)号：US06383785B1

    公开(公告)日：2002-05-07

    申请号：US08987348

    申请日：1997-12-09

    申请人： Rolf Mueller ,  Hans-Harald Sedlacek

    发明人： Rolf Mueller ,  Hans-Harald Sedlacek

    IPC分类号： C12N1566

    CPC分类号： A61K48/00 ,  A61K38/47 ,  C12N15/63 ,  C12N15/635

    摘要： The invention relates to a nucleic acid construct which constitutes a self-enhancing expression system and which comprises the following components: at least one first structural gene that encodes an active compound; at least one second structural gene that encodes a transcription factor protein; and at least one activation sequence comprised of at least one sequence that binds the transcription factor protein and at least one promoter sequence; wherein each activation sequence activates the expression of a structural gene and the expression of the transcription factor protein; and to the use of the nucleic acid construct for preparing a drug for treating diseases.

    Nucleic acid constructs containing hybrid promoters
    9.
    发明授权
    Nucleic acid constructs containing hybrid promoters 失效
    含有杂合启动子的核酸构建体

    公开(公告)号：US06576758B1

    公开(公告)日：2003-06-10

    申请号：US08936603

    申请日：1997-09-24

    申请人： Klaus-Heinrich Seifart ,  Rolf Mueller ,  Hans-Harald Sedlacek

    发明人： Klaus-Heinrich Seifart ,  Rolf Mueller ,  Hans-Harald Sedlacek

    IPC分类号： C12N1563

    CPC分类号： A61K48/00 ,  C12N15/63

    摘要： Nucleic acid constructs containing hybrid promoters for use in gene therapy and genetic manipulation. The invention relates to a nucleic acid construct for the precise, regulated expression of genes in host cells, which construct exhibits at least one mutation which inhibits the proper expression of the expressed gene and exhibits at least one additional second mutation which relieves the inhibition due to the first mutation, to an isolated cell which harbors the nucleic acid construct, and to the use of the nucleic acid construct for preparing pharmaceuticals and for treating diseases with excessive cell proliferation.

    摘要翻译： 含有用于基因治疗和遗传操作的杂合启动子的核酸构建体。 本发明涉及用于精确调节宿主细胞中基因表达的核酸构建体，其构建表现出至少一种抑制表达基因的适当表达的突变，并显示至少一种额外的第二突变，其减轻由于 第一个突变，含有核酸构建体的分离细胞，以及核酸构建体用于制备药物和用于治疗具有过度细胞增殖的疾病的用途。