公开(公告)号：US08308922B2

公开(公告)日：2012-11-13

申请号：US10587745

申请日：2005-01-26

申请人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

发明人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

IPC分类号： G01N27/26

CPC分类号： G01N27/403

摘要： Electrochemical transducer arrays are already known from the prior art. According to the invention, the transducer array is provided with at least one flexible, planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is disposed. The metal substrate and the insulator disposed thereon are structured in such a manner as to give metal areas which are electrically insulated the one from the other and which serve as sensor areas. The metal substrate used is self-contained so that the structured metal areas can be contacted from the lower side.

摘要翻译： 电化学换能器阵列从现有技术已经是已知的。 根据本发明，换能器阵列设置有至少一个柔性的平面金属基底，其上布置有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 金属基板和设置在其上的绝缘体以使得彼此电绝缘并用作传感器区域的金属区域的方式构造。 使用的金属基板是独立的，使得结构化金属区域可以从下侧接触。

公开(公告)号：US07591938B2

公开(公告)日：2009-09-22

申请号：US10537248

申请日：2003-11-28

申请人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

发明人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

IPC分类号： G01N27/26 ,  G01N33/487 ,  G01F1/64

CPC分类号： G01N33/5438 ,  G01N27/3277

摘要： Electrically charged molecules need to be transported in order to create a DNA sensor. The following measures are undertaken: base metals are introduced into a solution as a positive ion; negatively charged molecules are transported in an opposite direction and are enriched in the vicinity of the measuring electrodes. Binding-specific separation of the charged molecules can be achieved by forming metal layers on the measuring electrodes by depositing metal ions from the solution when a suitable potential is selected. Target DNA can more particularly be introduced into the vicinity of the catcher molecules on the measuring electrodes and non-specifically bound DNA can be removed. According to the associated device, the electrode arrangement may be associated with a sacrificial electrode made of more base metal than the material of the measuring electrodes. The measuring electrodes in particular may be made of noble metal, preferably gold, and the sacrificial electrode may be made of copper.

摘要翻译： 需要运送电荷分子以产生DNA传感器。 进行以下措施：将碱金属作为正离子引入溶液中; 带负电荷的分子以相反的方向输送并富集在测量电极附近。 带电分子的结合特异性分离可以通过在选择合适的电位时从溶液中沉积金属离子而在测量电极上形成金属层来实现。 目标DNA可以更特别地引入到测量电极上的捕获剂分子附近，并且可以除去非特异性结合的DNA。 根据相关装置，电极装置可以与由比测量电极的材料更多的基底金属制成的牺牲电极相关联。 测量电极特别可以由贵金属制成，优选由金制成，并且牺牲电极可以由铜制成。

公开(公告)号：US20060086625A1

公开(公告)日：2006-04-27

申请号：US10537248

申请日：2003-11-28

申请人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

发明人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

IPC分类号： G01N27/26 ,  G01N33/487 ,  G01F1/64

CPC分类号： G01N33/5438 ,  G01N27/3277

摘要： Electrically charged molecules need to be transported in order to create a DNA sensor. The following measures are undertaken: base metals are introduced into a solution as a positive ion; negatively charged molecules are transported in an opposite direction and are enriched in the vicinity of the measuring electrodes. Binding-specific separation of the charged molecules can be achieved by forming metal layers on the measuring electrodes by depositing metal ions from the solution when a suitable potential is selected. Target DNA can more particularly be introduced into the vicinity of the catcher molecules on the measuring electrodes and non-specifically bound DNA can be removed. According to the associated device, the electrode arrangement may be associated with a sacrificial electrode made of more base metal than the material of the measuring electrodes. The measuring electrodes in particular may be made of noble metal, preferably gold, and the sacrificial electrode may be made of cooper.

摘要翻译： 需要运送电荷分子以产生DNA传感器。 进行以下措施：将碱金属作为正离子引入溶液中; 带负电荷的分子以相反的方向输送并富集在测量电极附近。 带电分子的结合特异性分离可以通过在选择合适的电位时从溶液中沉积金属离子而在测量电极上形成金属层来实现。 目标DNA可以更特别地引入到测量电极上的捕获剂分子附近，并且可以除去非特异性结合的DNA。 根据相关装置，电极装置可以与由比测量电极的材料更多的基底金属制成的牺牲电极相关联。 测量电极特别可以由贵金属制成，优选由金制成，并且牺牲电极可以由铜制成。

公开(公告)号：US20090130658A1

公开(公告)日：2009-05-21

申请号：US11665380

申请日：2005-10-17

申请人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kuhn ,  Peter Paulicka ,  Manfred Stanzel

发明人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kuhn ,  Peter Paulicka ,  Manfred Stanzel

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L3/502723 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

摘要： A cartridge (card) having a system of microchannels and/or microcavities is used for automated DNA or protein analysis. In at least one embodiment, the microchannels or microcavities include geometrical structures for receiving dry reagents. For the purpose of industrial production, the cartridge is produced from a flat card support, e.g., by injection moulding. The reagents are spotted into the open channels, dried and then the channels are sealed by way of a film. A finished cartridge can thus be provided with a test sample and the fully automated measuring sequence can be initiated by inserting said cartridge into a read-out device.

摘要翻译： 具有微通道和/或微腔系统的药筒（卡）用于自动化DNA或蛋白质分析。 在至少一个实施例中，微通道或微腔包括用于接收干试剂的几何结构。 为了工业生产的目的，墨盒由平板卡支撑件制成，例如通过注射成型。 将试剂点入开放通道，干燥，然后通过膜封闭通道。 因此，成品墨盒可以设置有测试样品，并且可以通过将所述墨盒插入读出装置来启动完全自动测量顺序。

公开(公告)号：US20080283397A1

公开(公告)日：2008-11-20

申请号：US10587745

申请日：2005-01-26

申请人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

发明人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

IPC分类号： G01N33/487

CPC分类号： G01N27/403

摘要： Electrochemical transducer arrays are already known from the prior art. According to the invention, the transducer array is provided with at least one flexible, planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is disposed. The metal substrate and the insulator disposed thereon are structured in such a manner as to give metal areas which are electrically insulated the one from the other and which serve as sensor areas. The metal substrate used is self-contained so that the structured metal areas can be contacted from the lower side.

摘要翻译： 电化学换能器阵列从现有技术已经是已知的。 根据本发明，换能器阵列设置有至少一个柔性的平面金属基底，其上布置有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 金属基板和设置在其上的绝缘体以使得彼此电绝缘并用作传感器区域的金属区域的方式构造。 使用的金属基板是独立的，使得结构化金属区域可以从下侧接触。

公开(公告)号：US07851227B2

公开(公告)日：2010-12-14

申请号：US11665331

申请日：2005-10-17

申请人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kühn ,  Peter Paulicka ,  Manfred Stanzel

发明人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kühn ,  Peter Paulicka ,  Manfred Stanzel

IPC分类号： G01N1/10 ,  G01N35/00

CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L3/502723 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

摘要： Especially in order to carry out the so-called enzyme-coupled DNA hybridization test in a closed cartridge including a microfluid system, using stored dry reagents, the reagents must be dissolved in the microfluid system and transported into the measuring chamber directly before the measurement. During the dissolution of the reagents in water, air cushions that cannot reach the measuring chamber must absolutely be prevented from forming upstream of the reagent liquid. According to an embodiment of the invention, the liquid measuring sample and the liquid reagents are transported in such a way that the air cushion is directed into the waste line and the measuring sample and the reagents are then introduced into the measuring chamber without any air bubbles. In this way, measuring errors can be avoided.

摘要翻译： 特别是为了在包含微流体系统的封闭筒中进行所谓的酶偶联DNA杂交试验，使用储存的干试剂，试剂必须溶解在微流体系统中，并在测量前直接运送到测量室中。 在将试剂溶解在水中时，必须绝对防止不能到达测量室的气垫在试剂液体的上游形成。 根据本发明的一个实施例，液体测量样品和液体试剂以这样一种方式运输，使得气垫被引导到废液管线中，然后将测量样品和试剂引入测量室，而没有任何气泡 。 这样可以避免测量误差。

公开(公告)号：US20090136922A1

公开(公告)日：2009-05-28

申请号：US11665331

申请日：2005-10-17

申请人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kuhn ,  Peter Paulicka ,  Manfred Stanzel

发明人： Heike Barlag ,  Siegfried Birkle ,  Walter Gumbrecht ,  Daniela Kuhn ,  Peter Paulicka ,  Manfred Stanzel

IPC分类号： C12Q1/68 ,  C12M1/00

CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L3/502723 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

摘要： Especially in order to carry out the so-called enzyme-coupled DNA hybridisation test in a closed cartridge including a microfluid system, using stored dry reagents, the reagents must be dissolved in the microfluid system and transported into the measuring chamber directly before the measurement. During the dissolution of the reagents in water, air cushions that cannot reach the measuring chamber must absolutely be prevented from forming upstream of the reagent liquid. According to an embodiment of the invention, the liquid measuring sample and the liquid reagents are transported in such a way that the air cushion is directed into the waste line and the measuring sample and the reagents are then introduced into the measuring chamber without any air bubbles. In this way, measuring errors can be avoided.

摘要翻译： 特别是为了在包括微流体系统的封闭式筒体中进行所谓的酶偶联DNA杂交试验，使用储存的干试剂，试剂必须溶解在微流体系统中，并在测量前直接输送到测量室中。 在将试剂溶解在水中时，必须绝对防止不能到达测量室的气垫在试剂液体的上游形成。 根据本发明的一个实施例，液体测量样品和液体试剂以这样一种方式运输，使得气垫被引导到废液管线中，然后将测量样品和试剂引入测量室，而没有任何气泡 。 这样可以避免测量误差。

公开(公告)号：US08128803B2

公开(公告)日：2012-03-06

申请号：US11989938

申请日：2006-07-31

申请人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

发明人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

IPC分类号： G01N33/543 ,  G01N27/26 ,  G01N27/49

CPC分类号： C12Q1/001 ,  G01N27/3271 ,  Y10S435/817

摘要： A method follow the change in concentration of a redox-active substance, whereby suitable potentials for a reduction process or an oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of measuring phase and relaxation phase being determined in a suitable manner. A rapid relaxation of the concentration gradient is electrochemically forced so that the measurement can be carried out on simple transducer arrays. A device for carrying out the method includes a transducer array in addition to a suitable potentiostat. The transducer array can be formed of a planar metal substrate on which at least one flexible insulator is disposed, the metal surface and the insulator surface being firmly linked. The transducer array can also be formed of silicone-based CMOS structures.

摘要翻译： 一种遵循氧化还原活性物质浓度变化的方法，由此将适当的还原过程或氧化过程的电位施加到测量装置的工作电极上。 工作电极的电位是脉冲的，并且交替地产生测量相位和松弛相位，测量相位和弛豫阶段的脉冲长度以适当的方式确定。 浓度梯度的快速弛豫是电化学强制的，以便可以在简单的换能器阵列上进行测量。 用于执行该方法的装置包括除合适的恒电位仪之外的换能器阵列。 换能器阵列可以由其上设置有至少一个柔性绝缘体的平面金属基板形成，金属表面和绝缘体表面牢固地连接。 换能器阵列也可以由硅基CMOS结构形成。

公开(公告)号：US08105478B2

公开(公告)日：2012-01-31

申请号：US10587837

申请日：2005-01-26

申请人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

发明人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

IPC分类号： G01N27/26 ,  G01N33/53 ,  G01N33/487

CPC分类号： G01N27/3277 ,  G01N2333/916 ,  Y10T436/143333

摘要： In order to follow the change in concentration of a redox-active substance, potential suitable for a reducing process or oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of the measuring phase and relaxation phase being predetermined in a suitable manner. In this manner, a rapid relaxation of the concentration gradient is forced electrochemically so that the measurement can be carried out on simple transducer arrays. The device includes a transducer array in addition to a suitable potentiostat. The transducer array may include a planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is located. The array is generated by suitably structuring the substrate.

摘要翻译： 为了跟踪氧化还原活性物质的浓度变化，适用于还原过程或氧化过程的电位被施加到测量装置的工作电极。 工作电极的电位是脉冲的，交替地产生测量相位和弛豫阶段，测量相位和弛豫阶段的脉冲长度以合适的方式被预先确定。 以这种方式，电化学强迫浓缩梯度的快速松弛，使得可以在简单的换能器阵列上进行测量。 除了合适的恒电位仪之外，该装置还包括一个换能器阵列。 换能器阵列可以包括平面金属基底，其上定位有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 该阵列通过适当地构造基底而产生。

公开(公告)号：US20100155263A1

公开(公告)日：2010-06-24

申请号：US11989938

申请日：2006-07-31

申请人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

发明人： Heike Barlag ,  Walter Gumbrecht ,  Konrad Mund

IPC分类号： G01N33/543

CPC分类号： C12Q1/001 ,  G01N27/3271 ,  Y10S435/817

摘要： A method follow the change in concentration of a redox-active substance, whereby suitable potentials for a reduction process or an oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of measuring phase and relaxation phase being determined in a suitable manner. A rapid relaxation of the concentration gradient is electrochemically forced so that the measurement can be carried out on simple transducer arrays. A device for carrying out the method includes a transducer array in addition to a suitable potentiostat. The transducer array can be formed of a planar metal substrate on which at least one flexible insulator is disposed, the metal surface and the insulator surface being firmly linked. The transducer array can also be formed of silicone-based CMOS structures.

摘要翻译： 一种遵循氧化还原活性物质浓度变化的方法，由此将适当的还原过程或氧化过程的电位施加到测量装置的工作电极上。 工作电极的电位是脉冲的，并且交替地产生测量相位和松弛相位，测量相位和弛豫阶段的脉冲长度以适当的方式确定。 浓度梯度的快速弛豫是电化学强制的，以便可以在简单的换能器阵列上进行测量。 用于执行该方法的装置包括除合适的恒电位仪之外的换能器阵列。 换能器阵列可以由其上设置有至少一个柔性绝缘体的平面金属基板形成，金属表面和绝缘体表面牢固地连接。 换能器阵列也可以由硅基CMOS结构形成。