公开(公告)号：US06812023B1

公开(公告)日：2004-11-02

申请号：US09561205

申请日：2000-04-27

申请人： Henry Lamparski ,  Curtis Ruegg ,  Jean-Bernard Le Pecq ,  Di-Hewi Hsu ,  Jenq Yuan Yao

发明人： Henry Lamparski ,  Curtis Ruegg ,  Jean-Bernard Le Pecq ,  Di-Hewi Hsu ,  Jenq Yuan Yao

IPC分类号： C12N506

CPC分类号： H01J37/32431 ,  A61K2039/55555 ,  C12N5/0639 ,  C12N2500/90 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/24 ,  C23C16/4401 ,  C23C16/509 ,  G01N33/5432 ,  Y10S435/82

摘要： The present invention relates to methods of preparing biological material, for use in various experimental, diagnostic or therapeutic uses, including immunotherapy treatment or prophylaxy of tumors. More particularly, the present invention relates to methods of preparing membrane vesicles (in particular exosomes) released by various types of mammalian cells, comprising diafiltration and/or density cushion centrifugation. The invention also provides novel methods for characterizing and analyzing exosome preparations, which can be used in quality control assay for the purpose of pharmaceutical product production. The invention is suitable to produce pharmaceutical grade preparations of such membrane vesicles and to fully characterize said preparations, for use in human beings.

摘要翻译： 本发明涉及制备生物材料的方法，用于各种实验，诊断或治疗用途，包括免疫治疗或预防肿瘤。 更具体地说，本发明涉及制备由各种哺乳动物细胞释放的膜泡囊（特别是外来体）的方法，包括渗滤和/或密度缓冲离心。 本发明还提供用于表征和分析外来体制剂的新方法，其可用于药物产品生产目的的质量控制测定。 本发明适用于生产这种膜泡囊的药物级制剂，并且完全表征所述制剂用于人类。

公开(公告)号：US20050042272A1

公开(公告)日：2005-02-24

申请号：US10502333

申请日：2003-03-13

申请人： Yafei Hou ,  Du-Hwei Hsu ,  Anita Mehta-Damani ,  Henry Lamparski ,  Redro Paz ,  Jean-Bernard Le Pecq

发明人： Yafei Hou ,  Du-Hwei Hsu ,  Anita Mehta-Damani ,  Henry Lamparski ,  Redro Paz ,  Jean-Bernard Le Pecq

IPC分类号： C12N5/02 ,  A61K35/26 ,  A61K39/00 ,  A61K39/29 ,  A61K39/39 ,  A61K45/00 ,  C07K14/18 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/10 ,  A61K9/127 ,  C12P7/64 ,  A61K38/20

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/5154 ,  A61K2039/5158 ,  A61K2039/55555 ,  C12N5/0636 ,  C12N5/0639 ,  C12N2500/14 ,  C12N2501/20 ,  C12N2501/515 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2770/24222 ,  C12N2770/24234

摘要： The present invention relates to compositions comprising vesicles released from activated T lymphocytes, as well as to methods for their production and uses. Said vesicles contain a set of bioactive molecules which confer remarkable properties, such as antigen recognition, antigen presentation and other regulatory and effector functions. This invention also relates to methods for transferring or delivering antigenic molecules (e.g., peptides, peptide/MHC complexes, TCR or subunit thereof, etc.) to antigen presenting cells (APCs) using said vesicles, to induce specific immune responses, particularly specific CTL responses. The invention further relates to methods of delivering molecules selectively or specifically to target cells using said vesicles.

摘要翻译： 本发明涉及包含从活化的T淋巴细胞释放的囊泡的组合物，以及其生产和用途的方法。 所述囊泡含有一组生物活性分子，其具有显着的性质，例如抗原识别，抗原呈递和其它调节和效应子功能。 本发明还涉及使用所述囊泡将抗原分子（例如肽，肽/ MHC复合物，TCR或其亚基等）转移或递送至抗原呈递细胞（APC）以诱导特异性免疫应答，特别是特异性CTL 回应。 本发明还涉及使用所述囊泡选择性地或特异性地递送靶向细胞的方法。

公开(公告)号：US06585968B2

公开(公告)日：2003-07-01

申请号：US09898883

申请日：2001-07-02

申请人： Andrew S. Little ,  Daniel R. Henderson ,  Eric R. Schuur ,  Henry Lamparski

发明人： Andrew S. Little ,  Daniel R. Henderson ,  Eric R. Schuur ,  Henry Lamparski

IPC分类号： A61K4800

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/4715 ,  C12N15/63 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2830/002 ,  C12N2830/008

摘要： Adenovirus vectors replication specific for cells expressing &agr;-fetoprotein (AFP) and their methods of use are provided. By providing for a transcriptional initiating regulation dependent upon AFP expression, virus replication is restricted to target cells expressing AFP, particularly hepatocellular carcinoma cells. The adenovirus vectors can be used to detect and monitor samples for the presence of AFP-producing cells as well as to kill selectively malignant cells producing AFP.

公开(公告)号：US20060165658A1

公开(公告)日：2006-07-27

申请号：US11267275

申请日：2005-11-07

申请人： Andrew Little ,  Henry Lamparski ,  Daniel Henderson ,  Eric Schuur

发明人： Andrew Little ,  Henry Lamparski ,  Daniel Henderson ,  Eric Schuur

IPC分类号： C12N15/86 ,  C12N15/861 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10345 ,  C12N2830/008

摘要： Adenovirus vectors replication specific for cells expressing α-fetoprotein (AFP) and their methods of use are provided. By providing for a transcriptional initiating regulation dependent upon AFP expression, virus replication is restricted to target cells expressing AFP, particularly hepatocellular carcinoma cells. The adenovirus vectors can be used to detect and monitor samples for the presence of AFP-producing cells as well as to kill selectively malignant cells producing AFP.

公开(公告)号：US06254862B1

公开(公告)日：2001-07-03

申请号：US09033428

申请日：1998-03-02

申请人： Andrew S. Little ,  Daniel R. Henderson ,  Eric R. Schuur ,  Henry Lamparski

发明人： Andrew S. Little ,  Daniel R. Henderson ,  Eric R. Schuur ,  Henry Lamparski

IPC分类号： A61K4800

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/4715 ,  C12N15/63 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2830/002 ,  C12N2830/008

摘要： Adenovirus vectors replication specific for cells expressing &agr;-fetoprotein (AFP) and their methods of use are provided. By providing for a transcriptional initiating regulation dependent upon AFP expression, virus replication is restricted to target cells expressing AFP, particularly hepatocellular carcinoma cells. The adenovirus vectors can be used to detect and monitor samples for the presence of AFP-producing cells as well as to kill selectively malignant cells producing AFP.

摘要翻译： 提供了针对表达甲胎蛋白（AFP）的细胞特异性的腺病毒载体及其使用方法。 通过提供依赖于AFP表达的转录起始调节，病毒复制限于表达AFP的靶细胞，特别是肝细胞癌细胞。 腺病毒载体可用于检测和监测样品中是否存在产生AFP的细胞，以及选择性地杀死产生AFP的恶性细胞。