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公开(公告)号：US20140235457A1
公开(公告)日：2014-08-21
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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公开(公告)号：US20180051332A9
公开(公告)日：2018-02-22
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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公开(公告)号：US09926596B2
公开(公告)日：2018-03-27
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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4.发明授权公开(公告)号：US09187783B2
公开(公告)日：2015-11-17
申请号：US13824129
申请日：2011-10-04
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.
摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。
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公开(公告)号：US20100103416A1
公开(公告)日：2010-04-29
申请号：US12630683
申请日：2009-12-03
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US07636160B2
公开(公告)日：2009-12-22
申请号：US12321781
申请日：2009-01-26
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US07636159B2
公开(公告)日：2009-12-22
申请号：US12283957
申请日：2008-09-17
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
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公开(公告)号：US20090021735A1
公开(公告)日：2009-01-22
申请号：US12283957
申请日：2008-09-17
IPC分类号： G01N21/62
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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公开(公告)号：US20080105831A1
公开(公告)日：2008-05-08
申请号：US11749411
申请日：2007-05-16
IPC分类号： G01J1/58
CPC分类号： G01N21/6428 , G01N21/6452 , G01N21/648 , G01N2021/6432
摘要： An embodiment generally relates to a system for analysis of an analyte. The system can include a transparent substrate. The system also includes an excitation light source configured to induce an evanescent wave excitation of a fluorescently labeled molecule near the access to the transparent substrate and a detector for detecting the fluorescently labeled molecule.
摘要翻译： 实施方案一般涉及用于分析分析物的系统。 该系统可以包括透明基板。 该系统还包括激发光源，其被配置为在靠近透明衬底的位置处诱导荧光标记分子的ev逝波激发和用于检测荧光标记分子的检测器。
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公开(公告)号：US20130011842A1
公开(公告)日：2013-01-10
申请号：US13619298
申请日：2012-09-14
IPC分类号： G01N21/64
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译： 提供了一种用于检测标记珠粒的装置。 该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。
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