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    Cell culture of fetal liver in layered state in a partitioned micro-space
    1.
    发明授权
    Cell culture of fetal liver in layered state in a partitioned micro-space 有权
    在分层微空间中分层状态的胎肝细胞培养

    公开(公告)号：US09428721B2

    公开(公告)日：2016-08-30

    申请号：US12866581

    申请日：2009-02-05

    申请人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    发明人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/32 ,  G01N33/50

    CPC分类号： C12M23/22 ,  C12M23/12 ,  G01N33/5073

    摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

    摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。

    CELL CULTURE METHOD AND SCREENING METHOD
    2.
    发明申请
    CELL CULTURE METHOD AND SCREENING METHOD 有权
    细胞培养方法和筛选方法

    公开(公告)号：US20110045500A1

    公开(公告)日：2011-02-24

    申请号：US12866581

    申请日：2009-02-05

    申请人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    发明人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    IPC分类号： G01N33/53 ,  C12N5/071 ,  C12N5/079

    CPC分类号： C12M23/22 ,  C12M23/12 ,  G01N33/5073

    摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

    摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。

    CULTURE METHOD FOR CAUSING DIFFERENTIATION OF PLURIPOTENT MAMMALIAN CELLS
    3.
    发明申请
    CULTURE METHOD FOR CAUSING DIFFERENTIATION OF PLURIPOTENT MAMMALIAN CELLS 审中-公开
    培养方法，用于引起巨噬细胞的分化

    公开(公告)号：US20130071932A1

    公开(公告)日：2013-03-21

    申请号：US13636855

    申请日：2011-03-23

    申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

    发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

    IPC分类号： C12N5/071 ,  C12N5/095 ,  C12N5/076 ,  C12N5/0735

    CPC分类号： C12N5/0606 ,  C12M23/12 ,  C12N2502/28 ,  C12N2506/02 ,  C12N2535/10

    摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

    摘要翻译： 提供了通过使用其中形成有多个微室的细胞培养室，实现胚状体大小的控制并且可以在控制胚状体大小的状态下诱导分化的方法。 用于引起多能哺乳动物细胞分化的培养方法使用在培养表面上形成有多个微室（11）的细胞培养室（10）。 细胞培养室（10）具有由空间形成的培养面，其中微室（11）具有高度为10μm〜500μm，底面积为100μm2〜1mm2的空间结构。 引起多能哺乳动物细胞分化的培养方法包括通过使用细胞培养室（10）培养多能哺乳动物细胞以获得至少部分分化为内胚层谱系细胞的细胞群。