公开(公告)号：US09428721B2

公开(公告)日：2016-08-30

申请号：US12866581

申请日：2009-02-05

申请人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

发明人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/32 ,  G01N33/50

CPC分类号： C12M23/22 ,  C12M23/12 ,  G01N33/5073

摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。

公开(公告)号：US20130071932A1

公开(公告)日：2013-03-21

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/095 ,  C12N5/076 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12M23/12 ,  C12N2502/28 ,  C12N2506/02 ,  C12N2535/10

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

摘要翻译： 提供了通过使用其中形成有多个微室的细胞培养室，实现胚状体大小的控制并且可以在控制胚状体大小的状态下诱导分化的方法。 用于引起多能哺乳动物细胞分化的培养方法使用在培养表面上形成有多个微室（11）的细胞培养室（10）。 细胞培养室（10）具有由空间形成的培养面，其中微室（11）具有高度为10μm〜500μm，底面积为100μm2〜1mm2的空间结构。 引起多能哺乳动物细胞分化的培养方法包括通过使用细胞培养室（10）培养多能哺乳动物细胞以获得至少部分分化为内胚层谱系细胞的细胞群。

公开(公告)号：US20110104126A1

公开(公告)日：2011-05-05

申请号：US12992255

申请日：2009-05-13

申请人： Hideki Taniguchi ,  Yun-Wen Zheng

发明人： Hideki Taniguchi ,  Yun-Wen Zheng

IPC分类号： A61K35/407 ,  C12N5/071 ,  C12Q1/02 ,  A61P1/16

CPC分类号： C12N5/0672

摘要： The present invention provides a human hepatic stem cell which is sorted based on the presence or absence of the expression of at least one marker selected from the group consisting of CD318, CD90, CD66 and CD13. A method of preparing the human hepatic stem cell, a method of inducing differentiation of the same and a method of using the same are also provided.

摘要翻译： 本发明提供一种人肝干细胞，其根据是否存在选自CD318，CD90，CD66和CD13的至少一种标志物的表达进行分选。 还提供了制备人肝细胞的方法，诱导其分化的方法及其使用方法。

公开(公告)号：US10513685B2

公开(公告)日：2019-12-24

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12M1/32

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

公开(公告)号：US20110045500A1

公开(公告)日：2011-02-24

申请号：US12866581

申请日：2009-02-05

申请人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

发明人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

IPC分类号： G01N33/53 ,  C12N5/071 ,  C12N5/079

CPC分类号： C12M23/22 ,  C12M23/12 ,  G01N33/5073

摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。

公开(公告)号：US20110217725A1

公开(公告)日：2011-09-08

申请号：US13125896

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/07 ,  C12Q1/02

CPC分类号： C12N5/0062 ,  C12M23/12 ,  C12N2502/00 ,  C12N2503/02 ,  C12N2513/00

摘要： To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

摘要翻译： 提供包括各种供体的培养的活细胞的细胞培养试剂盒及其制造方法。 细胞培养试剂盒包括培养板和在其上培养的活细胞。 培养板包括多个微室（33），并且来自各种供体的活细胞粘附到多个微室（33）的表面。 具体而言，将来自各种供体的活细胞D1，D2和D3粘附到多个微室（33）。 在每个微室（33）中，可以培养衍生自一个供体的活细胞或来自各种供体的活细胞。 来自各种供体的活细胞作为整体在细胞培养试剂盒中附着和培养，这使得可以提供使用来自各种供体的细胞进行测试的细胞培养试剂盒。

公开(公告)号：US20110207964A1

公开(公告)日：2011-08-25

申请号：US12672327

申请日：2008-08-07

申请人： Michio Terasaka ,  Tetsuaki Fukushima ,  Hideki Taniguchi ,  Masaharu Jono

发明人： Michio Terasaka ,  Tetsuaki Fukushima ,  Hideki Taniguchi ,  Masaharu Jono

IPC分类号： C07C291/04 ,  C07C209/50

CPC分类号： C07C209/50 ,  C07C209/02 ,  C07C211/08 ,  C07C291/04

摘要： The present invention relates to a process for producing a tertiary amine in the presence of a catalyst containing copper and at least one element selected from the group consisting of elements belonging to Groups 2, 3, 7 and 12 of the Periodic Table (long form of the periodic table), said process including the steps of (a) reducing an amide compound in a hydrogen atmosphere; and (b) introducing a dialkyl amine containing a linear or branched alkyl group having 1 to 6 carbon atoms into a reaction product obtained in the step (a), and treating the reaction product with the dialkyl amine. The present invention provides a process for producing high-purity aliphatic tertiary amines containing a less amount of by-products by reducing aliphatic acid amides under moderate conditions using a chromium-free catalyst, as well as a process for producing amine derivatives such as amine oxide by using the aliphatic tertiary amines, with a good productivity in an economical manner.

摘要翻译： 本发明涉及在含有铜的催化剂和选自元素周期表第2,3,7和12族元素中的至少一种元素的存在下制备叔胺的方法（长形式 所述方法包括以下步骤：（a）在氢气氛中还原酰胺化合物; 和（b）将含有具有1至6个碳原子的直链或支链烷基的二烷基胺引入步骤（a）中获得的反应产物中，并用二烷基胺处理反应产物。 本发明提供了一种通过使用无铬催化剂在中等条件下还原脂肪酸酰胺以及生产胺衍生物如氧化胺的方法来生产含有少量副产物的高纯度脂族叔胺的方法 通过使用脂肪族叔胺，以经济的方式具有良好的生产率。

公开(公告)号：US20080292264A1

公开(公告)日：2008-11-27

申请号：US11660391

申请日：2005-08-05

申请人： Yoshifumi Kawaguchi ,  Hideki Taniguchi

发明人： Yoshifumi Kawaguchi ,  Hideki Taniguchi

IPC分类号： H04N5/93

CPC分类号： G11B27/3027 ,  G11B20/00086 ,  G11B20/1217 ,  G11B27/034 ,  H04N5/85 ,  H04N5/913 ,  H04N9/8063 ,  H04N9/8205

摘要： An authoring device includes: a separator for separating a video stream and an audio stream from the input stream; a multiplexer for multiplexing the video stream and the audio stream to generate content information and further generating control information for managing stream reproduction, generating, from content information and control information, incomplete disc image data which is a series of data basically based on a predetermined format and in which a part of reproduction control information defined by a predetermined format is missing, and further generating analysis information required for generating the missing part of the reproduction control information; and a disc image completing section for completing the incomplete disc image data by referencing the analysis information after generation of the incomplete disc image data.

摘要翻译： 创作设备包括：分离器，用于从输入流中分离视频流和音频流; 多路复用器，用于多路复用视频流和音频流以产生内容信息，并进一步产生用于管理流再现的控制信息，从内容信息和控制信息生成基于预定格式的一系列数据的不完整光盘图像数据 并且其中缺少由预定格式定义的再现控制信息的一部分，并且进一步产生生成再现控制信息的缺失部分所需的分析信息; 以及光盘图像完成部，用于通过在生成不完整的光盘图像数据之后参考分析信息来完成不完整的光盘图像数据。

公开(公告)号：US20050003529A1

公开(公告)日：2005-01-06

申请号：US10693712

申请日：2003-10-27

申请人： Hideki Taniguchi ,  Atsushi Suzuki

发明人： Hideki Taniguchi ,  Atsushi Suzuki

IPC分类号： A61K35/12 ,  A61P1/18 ,  C12N5/074 ,  C12Q1/68 ,  G01N33/50 ,  C12N5/08 ,  G01N33/53 ,  G01N33/567

CPC分类号： C12Q1/6881 ,  A61K35/12 ,  C12N5/0678 ,  C12N2503/02 ,  C12Q2600/158 ,  G01N33/5005

摘要： The present invention provides a method of separating a pancreatic stem cell from the pancreas of a mammal, a method of identifying a pancreatic stem cell of a mammal, and use of a pancreatic stem cell that can be separated or identified by this method. More particularly, the present invention provides a method of separating or identifying a pancreatic stem cell of a mammal, which includes analyzing the state of expression of 2 or more marker proteins selected from the group consisting of c-Met, c-Kit, CD45, TER119 and Flk-1, or a gene encoding the marker protein.

摘要翻译： 本发明提供了从哺乳动物的胰腺分离胰腺干细胞的方法，鉴定哺乳动物的胰腺干细胞的方法以及通过该方法分离或鉴定的胰干细胞的用途。 更具体地，本发明提供了分离或鉴定哺乳动物的胰腺干细胞的方法，其包括分析选自下组的2种或更多种标记蛋白的表达状态：c-Met，c-Kit，CD45， TER119和Flk-1，或编码标记蛋白的基因。

公开(公告)号：US6054620A

公开(公告)日：2000-04-25

申请号：US161485

申请日：1998-09-28

申请人： Hideki Taniguchi ,  Yasuyuki Mimura ,  Hiroshi Abe

发明人： Hideki Taniguchi ,  Yasuyuki Mimura ,  Hiroshi Abe

IPC分类号： B01J23/755 ,  C07B61/00 ,  C07C209/16 ,  C07C209/60 ,  C07C209/84 ,  C07C211/08 ,  C07C209/00

CPC分类号： C07C209/84

摘要： The present invention provides a process for producing a tertiary aliphatic amine having high quality having little non-amines such as esters and alcohols, which is less colored, and which can be converted into a derivative without turbidity.That is, the present invention provides the process which comprises the steps of adding at least one alkali substance of potassium hydroxide and sodium hydroxide or an aqueous solution thereof to the crude tertiary amine product mixture and distilling the mixture to obtain the tertiary amine having a high quality.In addition, the tertiary amine has the formula: R.sub.1 R.sub.2 N--R.sub.3, wherein R.sub.1 and R.sub.2 being a saturated or unsaturated hydrocarbon having 6 to 28 carbon atoms, R.sub.3 being a saturated or unsaturated hydrocarbon having 1 to 5 carbon atoms.

摘要翻译： 本发明提供了一种高品质的叔脂肪族胺的制造方法，其具有很少的非胺，如酯和醇，其颜色较少，并且可以转化为没有浊度的衍生物。 也就是说，本发明提供的方法包括以下步骤：向粗叔胺产物混合物中加入氢氧化钾和氢氧化钠的至少一种碱性物质或其水溶液，并蒸馏该混合物，得到具有高 质量。 此外，叔胺具有下式：R 1 R 2 N-R 3，其中R 1和R 2是具有6至28个碳原子的饱和或不饱和烃，R 3是具有1至5个碳原子的饱和或不饱和烃。