公开(公告)号：US4507233A

公开(公告)日：1985-03-26

申请号：US485375

申请日：1983-04-18

申请人： Hiraku Saito ,  Yasuo Suzuki ,  Katsumi Fujii ,  Kaoru Miyazaki ,  Takekazu Horio

发明人： Hiraku Saito ,  Yasuo Suzuki ,  Katsumi Fujii ,  Kaoru Miyazaki ,  Takekazu Horio

IPC分类号： C07K14/795 ,  G01N27/447 ,  B01D57/02 ,  C07G7/04

CPC分类号： G01N27/44726 ,  C07K14/795 ,  Y10S530/841

摘要： Colored proteins having a known molecular weight of 1800 to 321200 are prepared by coupling monomers of a colored protein having a known molecular weight or by coupling a colored protein having a known molecular weight with a colorless protein having a known molecular weight. At least two proteins are selected from the so prepared colored proteins and used as a colored molecular weight marker. This colored molecular weight marker is used for determination of the molecular weight of a protein having an unknown molecular weight, and can also be used as a reference protein for purification of a protein having a known molecular weight.

摘要翻译： 通过偶联具有已知分子量的有色蛋白质的单体或通过将具有已知分子量的着色蛋白质与具有已知分子量的无色蛋白质偶联而制备具有1800至321200已知分子量的有色蛋白质。 从如此制备的彩色蛋白质中选择至少两种蛋白质并用作有色分子量标记物。 该着色分子量标记用于测定分子量未知蛋白质的分子量，也可用作纯化具有已知分子量的蛋白质的参考蛋白质。

公开(公告)号：US4356072A

公开(公告)日：1982-10-26

申请号：US217917

申请日：1980-12-18

申请人： Hiraku Saito ,  Isamu Takagahara ,  Yasuo Suzuki ,  Tuyosi Fujita ,  Katsumi Fujii ,  Takekazu Horio

发明人： Hiraku Saito ,  Isamu Takagahara ,  Yasuo Suzuki ,  Tuyosi Fujita ,  Katsumi Fujii ,  Takekazu Horio

IPC分类号： A61B5/00 ,  B01D57/02 ,  C07K1/113 ,  C07K1/28 ,  C07K14/41 ,  C07K14/415 ,  G01N27/26 ,  G01N27/447 ,  G01N31/22

CPC分类号： G01N27/44795 ,  B01D57/02 ,  G01N27/44747

摘要： A group of compounds having the following general formula can be obtained by reacting chromoprotein and organic acid: ##STR1## wherein X is a chromophore, P is a protein, NH.sub.2 is mainly .epsilon.-amino group of lysine, n is a positive integer of 1 to 18, and m is a positive integer in the relation of m.ltoreq.n. This compound group has one to eighteen organic acids bonded thereto, and shows various isoelectric points in accordance with the number of the organic acids, but the respective isoelectric points are maintained constant. If such compounds each having an individual isoelectric point, are used as isoelectric point markers, it is possible to recognize accurately the position of isoelectric point only by a visual operation.

摘要翻译： 一组具有以下通式的化合物可以通过发色蛋白和有机酸反应获得：其中X是发色团，P是蛋白质，NH2主要是赖氨酸的ε-氨基，n是正整数1 18，m是m

公开(公告)号：US4107014A

公开(公告)日：1978-08-15

申请号：US754976

申请日：1976-12-28

申请人： Yasuo Suzuki ,  Isamu Takagahara ,  Tsuyoshi Fujita ,  Katsumi Fujii ,  Takekazu Horio

发明人： Yasuo Suzuki ,  Isamu Takagahara ,  Tsuyoshi Fujita ,  Katsumi Fujii ,  Takekazu Horio

IPC分类号： G01N27/26 ,  G01N27/447 ,  G01N30/04

CPC分类号： G01N27/44795 ,  G01N27/44726 ,  Y10T436/25375

摘要： Isoelectric point markers for a gel isoelectric separation are prepared to comprise at least two colored proteins of which the isoelectric point pHs are known and wherein the difference between the highest value and the lowest value among the said pHs is not less than 0.5. The markers are applied to analyze proteins in a gel isoelectric separation method. The method employing the said markers makes it easy to analyze proteins and shows an excellent performance.

摘要翻译： 制备用于凝胶等电点分离的等电点标记物，其包含其中等电点pH值已知的至少两种着色蛋白质，其中所述pH值中最高值和最低值之间的差不小于0.5。 应用标记物以凝胶等电分离方法分析蛋白质。 使用所述标记的方法使得易于分析蛋白质并显示出优异的性能。

公开(公告)号：US4258131A

公开(公告)日：1981-03-24

申请号：US54416

申请日：1979-07-03

申请人： Isamu Takagahara ,  Juniti Yamauti ,  Setsuko Yoshimura ,  Katsumi Fujii ,  Takekazu Horio

发明人： Isamu Takagahara ,  Juniti Yamauti ,  Setsuko Yoshimura ,  Katsumi Fujii ,  Takekazu Horio

IPC分类号： C12N9/99 ,  C12Q1/26 ,  C12Q1/32

CPC分类号： C12Q1/32

摘要： 1,6-Dihydro nicotinamide adenine dinucleotide inhibits both H-type lactic dehydrogenase and M-type lactic dehydrogenase which are isoenzymes of lactic dehydrogenase, but the degree of inhibition thereof against H-type considerably differs from that against M-type. A ratio of H-type lactic dehydrogenase to M-type lactic dehydrogenase in serum can be measured by utilizing the difference of inhibition degree. Therefore we can diagnose the organ with trouble.

摘要翻译： 1,6-二氢烟酰胺腺嘌呤二核苷酸抑制作为乳酸脱氢酶同工酶的H型乳酸脱氢酶和M型乳酸脱氢酶，但与H型相比，其抑制程度明显不同于M型。 利用抑制程度的差异可以测定血清中H型乳酸脱氢酶与M型乳酸脱氢酶的比例。 所以我们可以诊断器官有麻烦。

公开(公告)号：US5789186A

公开(公告)日：1998-08-04

申请号：US656934

申请日：1996-06-03

申请人： Hitoshi Takeshita ,  Hiroshi Morita ,  Akira Fujiwara ,  Yasutaka Kaneda ,  Tohru Watanabe ,  Taizou Hayashi ,  Kohji Uchida ,  Yushi Matsuo ,  Takekazu Horio

发明人： Hitoshi Takeshita ,  Hiroshi Morita ,  Akira Fujiwara ,  Yasutaka Kaneda ,  Tohru Watanabe ,  Taizou Hayashi ,  Kohji Uchida ,  Yushi Matsuo ,  Takekazu Horio

IPC分类号： C12Q1/48 ,  C12Q1/533 ,  G01N33/68 ,  C12Q1/34 ,  C12Q1/32 ,  C12Q1/42

CPC分类号： G01N33/6896 ,  C12Q1/533 ,  G01N2800/2871

摘要： A sample is treated with a phosphoglyceric acid mutase (PGAM) inhibitor (polythionic acid (salt) such as potassium tetrathionate) to inactivate the M-type isozyme activity and the B-type PGAM is quantified by determining PGAM isozymes by a rate assay. The B-type PGAM is a novel marker for cerebral apoplexy, and the diagnosis of cerebral apoplexy is enabled by assaying it.

摘要翻译： 用磷酸甘油酸变位酶（PGAM）抑制剂（多硫酸（盐）如四硫代硫酸钾）处理样品以失活M型同工酶活性，并通过速率测定法测定PGAM同功酶来定量B型PGAM。 B型PGAM是脑中风的新型标志物，通过测定脑卒中的诊断能力。