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    Modified reductase and its gene, and use thereof
    3.
    发明授权
    Modified reductase and its gene, and use thereof 失效
    修饰的还原酶及其基因及其用途

    公开(公告)号：US07163814B2

    公开(公告)日：2007-01-16

    申请号：US10608625

    申请日：2003-06-30

    申请人： Hiroyuki Asako ,  Masatoshi Shimizu

    发明人： Hiroyuki Asako ,  Masatoshi Shimizu

    IPC分类号： C12N9/02 ,  C12N15/00 ,  C12P21/04 ,  C12Q1/34 ,  C12Q1/26 ,  C12Q1/00 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12N9/0036 ,  C12P7/62

    摘要： There is provided a reductase characterized by (i) an amino acid sequence of SEQ ID NO:1 having a substitution at amino acid position 54 or 104 or at both of the amino acid positions 54 and 104, or (ii) an amino acid sequence defined in (a) having further deletion, substitution, or addition of an amino acid or acids and the like, which reductase is provided with good heat stability and selectivity.

    摘要翻译： 提供了一种还原酶，其特征在于：（i）SEQ ID NO：1的氨基酸序列在氨基酸位置54或104或氨基酸位置54和104两者具有取代，或（ii）氨基酸序列 定义为（a）进一步缺失，取代或添加氨基酸或酸等，该还原酶具有良好的热稳定性和选择性。

    Modified reductase and its gene
    4.
    发明授权
    Modified reductase and its gene 失效
    修饰的还原酶及其基因

    公开(公告)号：US07135318B2

    公开(公告)日：2006-11-14

    申请号：US10608533

    申请日：2003-06-30

    申请人： Hiroyuki Asako ,  Masatoshi Shimizu

    发明人： Hiroyuki Asako ,  Masatoshi Shimizu

    IPC分类号： C12N9/02 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/26 ,  C12P21/04 ,  C07H21/04

    CPC分类号： C12N9/0036 ,  C12P7/42 ,  C12P7/62

    摘要： There are disclosed a reductase having good heat stability, which resulted in decrease in reaction time and improvement of reaction efficiency. Specifically, disclosed is an enzyme having an amino acid sequence of SEQ ID NO:1 in which at least one of the amino acids of the positions 245 and 271 of the amino acid sequence of SEQ ID NO:1 is replaced with another amino acid(s); a polynucleotide having a nucleotide sequence that encodes the amino acid sequence of said reductase; a vector containing said polynucleotide; a transformant containing said polynucleotide or said vector, and the like.

    摘要翻译： 公开了具有良好的热稳定性的还原酶，导致反应时间的降低和反应效率的提高。 具体地，公开了具有SEQ ID NO：1的氨基酸序列的酶，其中SEQ ID NO：1的氨基酸序列的位置245和271的至少一个氨基酸被替换为另一个氨基酸（ s）; 具有编码所述还原酶的氨基酸序列的核苷酸序列的多核苷酸; 含有所述多核苷酸的载体; 含有所述多核苷酸或所述载体的转化体等。

    Modified reductase and its gene, and use thereof
    5.
    发明申请
    Modified reductase and its gene, and use thereof 失效
    修饰的还原酶及其基因及其用途

    公开(公告)号：US20050191738A1

    公开(公告)日：2005-09-01

    申请号：US10608625

    申请日：2003-06-30

    申请人： Hiroyuki Asako ,  Masatoshi Shimizu

    发明人： Hiroyuki Asako ,  Masatoshi Shimizu

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P7/62 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/0036 ,  C12P7/62

    摘要： There is provided a reductase characterized by (i) an amino acid sequence of SEQ ID NO:1 having a substitution at amino acid position 54 or 104 or at both of the amino acid positions 54 and 104, or (ii) an amino acid sequence defined in (a) having further deletion, substitution, or addition of an amino acid or acids and the like, which reductase is provided with good heat stability and selectivity.

    摘要翻译： 提供了一种还原酶，其特征在于：（i）SEQ ID NO：1的氨基酸序列在氨基酸位置54或104或氨基酸位置54和104两者具有取代，或（ii）氨基酸序列 定义为（a）进一步缺失，取代或添加氨基酸或酸等，该还原酶具有良好的热稳定性和选择性。

    PROCESS FOR PRODUCING SULFUR-CONTAINING ALPHA-AMINO ACID COMPOUND
    6.
    发明申请
    PROCESS FOR PRODUCING SULFUR-CONTAINING ALPHA-AMINO ACID COMPOUND 审中-公开
    生产含硫酸阿氨酸酸化合物的方法

    公开(公告)号：US20130157320A1

    公开(公告)日：2013-06-20

    申请号：US13703287

    申请日：2011-06-30

    申请人： Hiroyuki Asako

    发明人： Hiroyuki Asako

    IPC分类号： C12P13/04

    CPC分类号： C12P13/04 ,  C12P13/12

    摘要： The present invention provides a novel process for producing a sulfur-containing α-amino acid compound such as methionine. A process for producing a sulfur-containing α-amino acid compound represented by the formula (2): wherein R1 represents hydrogen, an alkyl group having 1 to 8 carbon atoms, or an aryl group having 6 to 20 carbon atoms; comprising: a first step of culturing a microorganism capable of converting a sulfur-containing amino alcohol compound represented by the formula (1): wherein R1 is the same as defined above into the corresponding sulfur-containing α-amino acid compound in a culture medium containing a lower aliphatic alcohol to prepare a microbial cell of the microorganism; and a second step of reacting the sulfur-containing amino alcohol compound with the microbial cell of the microorganism obtained in the first step or a processed product of the microbial cell.

    摘要翻译： 本发明提供了一种生产含硫α-氨基酸化合物如蛋氨酸的新方法。 一种制备由式（2）表示的含硫α-氨基酸化合物的方法：其中R1表示氢，具有1至8个碳原子的烷基或具有6至20个碳原子的芳基; 包括：培养能够将由式（1）表示的含硫氨基醇化合物（其中R1与上述定义相同）的微生物培养到培养基中的相应含硫α-氨基酸化合物中的第一步骤 含有低级脂族醇以制备微生物的微生物细胞; 和使含硫氨基醇化合物与第一步获得的微生物的微生物细胞或微生物细胞的加工产物反应的第二步骤。

    Reductase gene and use thereof
    7.
    发明申请
    Reductase gene and use thereof 失效
    还原酶基因及其用途

    公开(公告)号：US20070072278A1

    公开(公告)日：2007-03-29

    申请号：US11516374

    申请日：2006-09-06

    申请人： Hiroyuki Asako

    发明人： Hiroyuki Asako

    IPC分类号： C12P7/42 ,  C07H21/04 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12P7/42 ,  C12N9/0008

    摘要： Provided are genes coding a protein having an ability of asymmetrically reducing a 2-oxo-4-phenylbutyrate to produce a (R)-2-hydroxy-4-phenylbutyrate advantageously from the industrial standpoint, the protein, and methods of producing a (R)-2-hydroxy-4-phenylbutyrate using them. Genes having a base sequence coding an amino acid sequence shown in SEQ ID NO:1, or a base sequence shown by a base sequence shown in SEQ ID NO:2.

    摘要翻译： 提供的是编码具有不对称还原2-氧代-4-苯基丁酸酯的能力的蛋白质的基因，从工业的角度有利地产生（R）-2-羟基-4-苯基丁酸酯，蛋白质和产生（R ）-2-羟基-4-苯基丁酸酯。 具有编码SEQ ID NO：1所示的氨基酸序列的碱基序列的基因或由SEQ ID NO：2所示的碱基序列显示的碱基序列。

    PROCESS FOR PRODUCING OPTICALLY-ACTIVE ORTHO-SUBSTITUTED MANDELIC ACID COMPOUND
    8.
    发明申请
    PROCESS FOR PRODUCING OPTICALLY-ACTIVE ORTHO-SUBSTITUTED MANDELIC ACID COMPOUND 审中-公开
    用于生产光学活性的取代的马来酸化合物的方法

    公开(公告)号：US20110033905A1

    公开(公告)日：2011-02-10

    申请号：US12863902

    申请日：2009-01-21

    申请人： Hiroyuki Asako

    发明人： Hiroyuki Asako

    IPC分类号： C12P7/42

    CPC分类号： C12P7/42 ,  C12P7/62 ,  C12P13/02

    摘要： The present invention provides a process of asymmetrically reducing an ortho-substituted phenylglyoxalic acid compound to produce a corresponding optically-active ortho-substituted mandelic acid compound by using a microorganism having an ability of reducing the ortho-substituted phenylglyoxalic acid compound into the optically-active ortho-substituted mandelic acid compound, or a treated product thereof.

    摘要翻译： 本发明提供了通过使用具有将邻位取代的苯乙醛酸化合物还原成光学活性的能力的微生物，不对称地还原邻位取代的苯乙醛酸化合物以产生相应的光学活性邻位取代的扁桃酸化合物的方法 邻位取代的扁桃酸化合物或其处理产物。

    REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME
    9.
    发明申请
    REDUCTASE, GENE THEREOF AND METHOD OF USING THE SAME 失效
    还原酶，其基因及其使用方法

    公开(公告)号：US20100112701A1

    公开(公告)日：2010-05-06

    申请号：US12532099

    申请日：2008-03-24

    申请人： Hiroyuki Asako

    发明人： Hiroyuki Asako

    IPC分类号： C12N15/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/00 ,  C12N1/21 ,  C07K14/195 ,  C07C29/00 ,  C07C59/01

    CPC分类号： C12P13/02 ,  C12N9/0004 ,  C12P7/42 ,  C12P7/62

    摘要： The present invention relates to a protein which can asymmetrically reduce an ortho-substituted phenylglyoxalic acid compound to produce an aide or ester compound of corresponding optically-active ortho-substituted mandelic acid compound with a good optical yield, a DNA encoding the protein, a process for producing the protein from the DNA, and a process for asymmetrically reducing an ortho-substituted phenylglyoxalic acid compound to produce a corresponding optically-active ortho-substituted mandelic acid compound.

    摘要翻译： 本发明涉及可以不对称地还原邻位取代的苯乙醛酸化合物以产生具有良好光学收率的相应的光学活性邻位取代的扁桃酸化合物的辅剂或酯化合物的蛋白质，编码蛋白质的DNA， 用于从DNA产生蛋白质，以及用于不对称地还原邻位取代的苯乙醛酸化合物以产生相应的光学活性邻位取代的扁桃酸化合物的方法。

    PROCESS FOR PRODUCING METHIONINE
    10.
    发明申请
    PROCESS FOR PRODUCING METHIONINE 审中-公开
    生产甲硫氨酸的方法

    公开(公告)号：US20130143279A1

    公开(公告)日：2013-06-06

    申请号：US13700891

    申请日：2011-05-30

    申请人： Koji Hagiya ,  Hiroyuki Asako

    发明人： Koji Hagiya ,  Hiroyuki Asako

    IPC分类号： C07C319/20 ,  C07C319/18

    CPC分类号： C07C319/20 ,  C07C319/18 ,  C12N1/32 ,  C12P13/12 ,  C07C323/25 ,  C07C323/58

    摘要： The present invention relates to a process for producing methionine, comprising a first step of reacting 2-amino-3-buten-1-ol with methanethiol, and a second step of oxidizing 2-amino-4-methylthio-1-butanol obtained in the first step. The present invention also relates to a process for producing 2-amino-4-methylthio-1-butanol, comprising a step of reacting 2-amino-3-buten-1-ol with methanethiol.

    摘要翻译： 本发明涉及一种生产甲硫氨酸的方法，包括使2-氨基-3-丁烯-1-醇与甲硫醇反应的第一步骤，以及将2-氨基-4-甲硫基-1-丁醇氧化的第二步骤 第一步。 本发明还涉及2-氨基-4-甲硫基-1-丁醇的制备方法，其包括使2-氨基-3-丁烯-1-醇与甲硫醇反应的步骤。