公开(公告)号：US5710016A

公开(公告)日：1998-01-20

申请号：US419009

申请日：1995-04-07

申请人： Hiroyuki Izu ,  Masanori Mitta ,  Ikunoshin Kato

发明人： Hiroyuki Izu ,  Masanori Mitta ,  Ikunoshin Kato

IPC分类号： C12N15/09 ,  C12N9/78 ,  C12N9/80 ,  C12N15/55 ,  C12R1/19 ,  C12R1/645 ,  C12N15/29 ,  C12N15/52

CPC分类号： C12N9/80

摘要： An almond N-glycosidase gene and genes associated therewith are described. Vectors integrated such genes therein, recombinant microorganisms transformed with said vectors, and a process for preparing the almond N-glycosidase using said recombinant microorganisms are also described.

摘要翻译： 描述杏仁N-糖苷酶基因及其相关基因。 还描述了在其中整合这些基因的载体，用所述载体转化的重组微生物，以及使用所述重组微生物制备杏仁N-糖苷酶的方法。

公开(公告)号：US20070128212A1

公开(公告)日：2007-06-07

申请号：US10580604

申请日：2004-11-26

申请人： Mutsumi Sano ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

发明人： Mutsumi Sano ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

IPC分类号： A61K36/752 ,  A61K36/61 ,  A61K36/16 ,  A61K36/42 ,  A61K36/28 ,  A61K36/02

CPC分类号： A61K31/201 ,  A23L33/105 ,  A61K8/97 ,  A61K36/03 ,  A61K36/16 ,  A61K36/28 ,  A61K36/282 ,  A61K36/42 ,  A61K36/61 ,  A61K36/75 ,  A61K2800/782 ,  A61Q19/02 ,  C07C59/42

摘要： The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral/alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

摘要翻译： 本发明提供了抑制神经酰胺酶活性，特别是中性/碱性神经酰胺糖酶活性的神经酰胺酶活性抑制剂，其特征在于，所述抑制剂包含作为活性成分的来自至少一种选自属于 银杏科，属于葫芦科的植物，属于芸香科的植物，属于ari科的植物，属于桃金娘科的植物和属于菊科的植物，以及各自包含抑制剂的药物，准药物，化妆品和食品。

公开(公告)号：US06428999B1

公开(公告)日：2002-08-06

申请号：US09160036

申请日：1998-09-25

申请人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

发明人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

IPC分类号： C12N980

CPC分类号： C07H15/10 ,  C07F9/10 ,  C07H15/04 ,  C12N9/80 ,  C12P13/02 ,  C12P19/26

摘要： The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

摘要翻译： 本发明涉及具有宽底物特异性的新型鞘脂神经酰胺N-脱酰酶（SCDase）; 使用可用于医药，碳水化合物工程，细胞工程等领域的SCDase酶促产生溶血神经鞘脂或鞘脂衍生物的方法; 通过该制造方法得到的溶血鞘脂或鞘脂衍生物; 编码具有可用于神经鞘脂技术的SCDase活性的多肽的基因; 使用引入了该基因的转化体的工业生产具有SCD酶脱酰酶活性的多肽的方法及其重组多肽; 与基因杂交的探针或引物; 以及特异性结合多肽的抗体或其片段。

公开(公告)号：US07045322B1

公开(公告)日：2006-05-16

申请号：US09647135

申请日：1999-03-30

申请人： Toyohisa Kurita ,  Hiroyuki Izu ,  Mutsumi Sano ,  Ikunoshin Kato

发明人： Toyohisa Kurita ,  Hiroyuki Izu ,  Mutsumi Sano ,  Ikunoshin Kato

IPC分类号： C12N9/00

CPC分类号： C12P13/001

摘要： A process for producing lysosphingolipids by using an enzyme capable of specifically hydrolyzing the acid amide bond between a sphingoid and a fatty acid in a sphingolipid, characterized in that the enzyme reaction is carried out in a two-phase system liquid reaction mixture containing at least an organic solvent forming an aqueous phase and a separation phase in the presence of, if desired, a surfactant.

摘要翻译： 通过使用能够特异性水解鞘脂中的鞘氨醇和脂肪酸之间的酰胺键的酶来生产溶血鞘脂的方法，其特征在于，所述酶反应在含有至少一种 在需要时，在表面活性剂的存在下形成水相和分离相的有机溶剂。

公开(公告)号：US5824503A

公开(公告)日：1998-10-20

申请号：US672564

申请日：1996-06-28

申请人： Yoko Kurome ,  Hiroyuki Izu ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

发明人： Yoko Kurome ,  Hiroyuki Izu ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

IPC分类号： C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  C12N15/00

CPC分类号： C07K14/36

摘要： An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof by gene recombinant technology.

摘要翻译： 具有编码具有神经酰胺糖内切酶激活剂活性的多肽的序列的分离的DNA或其功能上等同的变体; 以及通过基因重组技术产生具有神经酰胺糖内切酶激活剂活性或功能等同变体的多肽的方法。

公开(公告)号：US06821761B2

公开(公告)日：2004-11-23

申请号：US10150068

申请日：2002-05-20

申请人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

发明人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

IPC分类号： C12P1300

CPC分类号： C07H15/10 ,  C07F9/10 ,  C07H15/04 ,  C12N9/80 ,  C12P13/02 ,  C12P19/26

摘要： The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

公开(公告)号：US20080268073A1

公开(公告)日：2008-10-30

申请号：US12147798

申请日：2008-06-27

申请人： Mutsumi SANO ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

发明人： Mutsumi SANO ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

IPC分类号： A61K36/00 ,  C07C233/02 ,  C12N9/99

CPC分类号： A61K31/201 ,  A23L33/105 ,  A61K8/97 ,  A61K36/03 ,  A61K36/16 ,  A61K36/28 ,  A61K36/282 ,  A61K36/42 ,  A61K36/61 ,  A61K36/75 ,  A61K2800/782 ,  A61Q19/02 ,  C07C59/42

摘要： The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral/alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

摘要翻译： 本发明提供了抑制神经酰胺酶活性，特别是中性/碱性神经酰胺糖酶活性的神经酰胺酶活性抑制剂，其特征在于，所述抑制剂包含作为活性成分的来自至少一种选自属于 银杏科，属于葫芦科的植物，属于芸香科的植物，属于ari科的植物，属于桃金娘科的植物和属于菊科的植物，以及各自包含抑制剂的药物，准药物，化妆品和食品。

公开(公告)号：US5795765A

公开(公告)日：1998-08-18

申请号：US672571

申请日：1996-06-28

申请人： Hiroyuki Izu ,  Yoko Kurome ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

发明人： Hiroyuki Izu ,  Yoko Kurome ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

IPC分类号： C12N9/24 ,  C12N15/56 ,  C12N1/20 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12Y302/01123 ,  C12N9/2402

摘要： An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, and a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof by gene recombinant technology.

摘要翻译： 具有编码具有神经酰胺糖内切酶活性或其功能等同变体的多肽的序列的分离的DNA，以及通过基因重组技术产生具有神经酰胺糖内切酶活性或功能等同变体的多肽的方法。

公开(公告)号：US20120270309A1

公开(公告)日：2012-10-25

申请号：US13389479

申请日：2010-08-18

申请人： Tateki Arai ,  Osamu Takeda ,  Hiroyuki Izu ,  Hiroyuki Mukai

发明人： Tateki Arai ,  Osamu Takeda ,  Hiroyuki Izu ,  Hiroyuki Mukai

IPC分类号： C12M1/00

CPC分类号： B01L7/52 ,  B01L3/50825 ,  B01L2200/147 ,  B01L2300/1827

摘要： A thermal cycler for incubating reaction mixture, the thermal cycler including:(1) a heat block for holding and heating reaction containers which contain the reaction mixture, the heat block being a double-layered structure of a lower layer heat block and an upper layer heat block; and (2) a thermal control means for controlling a temperature of the lower layer heat block and a temperature of the upper layer heat block independently and respectively and keeping the temperature of the upper layer heat block higher than the temperature of the lower layer heat block while incubating the reaction mixture. Since the thermal cycler prevents the occurrence of the condensation of water or other components of the reaction mixture in the reaction container and the thermal differences based on the installation positions of the reaction containers, the PCR method and the other enzyme reaction can be performed in a good repeatability.

摘要翻译： 一种用于温育反应混合物的热循环仪，所述热循环仪包括：（1）用于保持和加热含有反应混合物的反应容器的热块，所述加热块是下层加热块和上层的双层结构 热块; 和（2）分别独立地分别控制下层热块的温度和上层热块的温度的热控制装置，并且保持上层热块的温度高于下层热块的温度 同时孵育反应混合物。 由于热循环仪防止反应容器中反应混合物的水或其它组分的冷凝发生，以及基于反应容器的安装位置的热差异，PCR法和其它酶反应可以在 良好的重复性。