公开(公告)号：US07045322B1

公开(公告)日：2006-05-16

申请号：US09647135

申请日：1999-03-30

申请人： Toyohisa Kurita ,  Hiroyuki Izu ,  Mutsumi Sano ,  Ikunoshin Kato

发明人： Toyohisa Kurita ,  Hiroyuki Izu ,  Mutsumi Sano ,  Ikunoshin Kato

IPC分类号： C12N9/00

CPC分类号： C12P13/001

摘要： A process for producing lysosphingolipids by using an enzyme capable of specifically hydrolyzing the acid amide bond between a sphingoid and a fatty acid in a sphingolipid, characterized in that the enzyme reaction is carried out in a two-phase system liquid reaction mixture containing at least an organic solvent forming an aqueous phase and a separation phase in the presence of, if desired, a surfactant.

摘要翻译： 通过使用能够特异性水解鞘脂中的鞘氨醇和脂肪酸之间的酰胺键的酶来生产溶血鞘脂的方法，其特征在于，所述酶反应在含有至少一种 在需要时，在表面活性剂的存在下形成水相和分离相的有机溶剂。

公开(公告)号：US20070128212A1

公开(公告)日：2007-06-07

申请号：US10580604

申请日：2004-11-26

申请人： Mutsumi Sano ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

发明人： Mutsumi Sano ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

IPC分类号： A61K36/752 ,  A61K36/61 ,  A61K36/16 ,  A61K36/42 ,  A61K36/28 ,  A61K36/02

CPC分类号： A61K31/201 ,  A23L33/105 ,  A61K8/97 ,  A61K36/03 ,  A61K36/16 ,  A61K36/28 ,  A61K36/282 ,  A61K36/42 ,  A61K36/61 ,  A61K36/75 ,  A61K2800/782 ,  A61Q19/02 ,  C07C59/42

摘要： The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral/alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

摘要翻译： 本发明提供了抑制神经酰胺酶活性，特别是中性/碱性神经酰胺糖酶活性的神经酰胺酶活性抑制剂，其特征在于，所述抑制剂包含作为活性成分的来自至少一种选自属于 银杏科，属于葫芦科的植物，属于芸香科的植物，属于ari科的植物，属于桃金娘科的植物和属于菊科的植物，以及各自包含抑制剂的药物，准药物，化妆品和食品。

公开(公告)号：US20080268073A1

公开(公告)日：2008-10-30

申请号：US12147798

申请日：2008-06-27

申请人： Mutsumi SANO ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

发明人： Mutsumi SANO ,  Hiroyuki Izu ,  Mitsuo Imamura ,  Motoko Okamoto ,  Toyohisa Kurita ,  Ikunoshin Kato

IPC分类号： A61K36/00 ,  C07C233/02 ,  C12N9/99

CPC分类号： A61K31/201 ,  A23L33/105 ,  A61K8/97 ,  A61K36/03 ,  A61K36/16 ,  A61K36/28 ,  A61K36/282 ,  A61K36/42 ,  A61K36/61 ,  A61K36/75 ,  A61K2800/782 ,  A61Q19/02 ,  C07C59/42

摘要： The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral/alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

摘要翻译： 本发明提供了抑制神经酰胺酶活性，特别是中性/碱性神经酰胺糖酶活性的神经酰胺酶活性抑制剂，其特征在于，所述抑制剂包含作为活性成分的来自至少一种选自属于 银杏科，属于葫芦科的植物，属于芸香科的植物，属于ari科的植物，属于桃金娘科的植物和属于菊科的植物，以及各自包含抑制剂的药物，准药物，化妆品和食品。

公开(公告)号：US06821761B2

公开(公告)日：2004-11-23

申请号：US10150068

申请日：2002-05-20

申请人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

发明人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

IPC分类号： C12P1300

CPC分类号： C07H15/10 ,  C07F9/10 ,  C07H15/04 ,  C12N9/80 ,  C12P13/02 ,  C12P19/26

摘要： The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

公开(公告)号：US06428999B1

公开(公告)日：2002-08-06

申请号：US09160036

申请日：1998-09-25

申请人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

发明人： Makoto Ito ,  Toyohisa Kurita ,  Katsuhiro Kita ,  Noriyuki Sueyoshi ,  Susumu Mitsutake ,  Masanori Fujita ,  Nozomu Okino ,  Hiroyuki Izu ,  Ikunoshin Kato

IPC分类号： C12N980

CPC分类号： C07H15/10 ,  C07F9/10 ,  C07H15/04 ,  C12N9/80 ,  C12P13/02 ,  C12P19/26

摘要： The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.

摘要翻译： 本发明涉及具有宽底物特异性的新型鞘脂神经酰胺N-脱酰酶（SCDase）; 使用可用于医药，碳水化合物工程，细胞工程等领域的SCDase酶促产生溶血神经鞘脂或鞘脂衍生物的方法; 通过该制造方法得到的溶血鞘脂或鞘脂衍生物; 编码具有可用于神经鞘脂技术的SCDase活性的多肽的基因; 使用引入了该基因的转化体的工业生产具有SCD酶脱酰酶活性的多肽的方法及其重组多肽; 与基因杂交的探针或引物; 以及特异性结合多肽的抗体或其片段。

公开(公告)号：US5824503A

公开(公告)日：1998-10-20

申请号：US672564

申请日：1996-06-28

申请人： Yoko Kurome ,  Hiroyuki Izu ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

发明人： Yoko Kurome ,  Hiroyuki Izu ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

IPC分类号： C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  C12N15/00

CPC分类号： C07K14/36

摘要： An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing endoglycoceramidase activator activity or functionally equivalent variants thereof by gene recombinant technology.

摘要翻译： 具有编码具有神经酰胺糖内切酶激活剂活性的多肽的序列的分离的DNA或其功能上等同的变体; 以及通过基因重组技术产生具有神经酰胺糖内切酶激活剂活性或功能等同变体的多肽的方法。

公开(公告)号：US5795765A

公开(公告)日：1998-08-18

申请号：US672571

申请日：1996-06-28

申请人： Hiroyuki Izu ,  Yoko Kurome ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

发明人： Hiroyuki Izu ,  Yoko Kurome ,  Yoshiya Izumi ,  Mutsumi Sano ,  Ikunoshin Kato ,  Makoto Ito

IPC分类号： C12N9/24 ,  C12N15/56 ,  C12N1/20 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12Y302/01123 ,  C12N9/2402

摘要： An isolated DNA having a sequence encoding a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof, and a method for producing a polypeptide possessing endoglycoceramidase activity or functionally equivalent variants thereof by gene recombinant technology.

摘要翻译： 具有编码具有神经酰胺糖内切酶活性或其功能等同变体的多肽的序列的分离的DNA，以及通过基因重组技术产生具有神经酰胺糖内切酶活性或功能等同变体的多肽的方法。

公开(公告)号：US06787359B1

公开(公告)日：2004-09-07

申请号：US09743354

申请日：2001-01-09

申请人： Mitsuhiro Ueno ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

发明人： Mitsuhiro Ueno ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

IPC分类号： C12N1563

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/10 ,  C12N2810/80 ,  C12N2810/851 ,  C12N2810/859

摘要： Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.

摘要翻译： 通过使用逆转录病毒将基因转移到靶细胞中的改进方法，其中基因转移效率得到改善，并且通过将逆转录病毒结合到固定在载体上并具有结合逆转录病毒活性的功能物质来有效转化靶细胞 随后洗涤; 使用能够特异性识别细胞的抗体，层粘连蛋白或富甘糖型糖链作为具有与靶细胞结合的活性的物质; 预处理靶细胞以使转铁蛋白受体失活，或将新的官能团引入功能物质。

公开(公告)号：US5710016A

公开(公告)日：1998-01-20

申请号：US419009

申请日：1995-04-07

申请人： Hiroyuki Izu ,  Masanori Mitta ,  Ikunoshin Kato

发明人： Hiroyuki Izu ,  Masanori Mitta ,  Ikunoshin Kato

IPC分类号： C12N15/09 ,  C12N9/78 ,  C12N9/80 ,  C12N15/55 ,  C12R1/19 ,  C12R1/645 ,  C12N15/29 ,  C12N15/52

CPC分类号： C12N9/80

摘要： An almond N-glycosidase gene and genes associated therewith are described. Vectors integrated such genes therein, recombinant microorganisms transformed with said vectors, and a process for preparing the almond N-glycosidase using said recombinant microorganisms are also described.

摘要翻译： 描述杏仁N-糖苷酶基因及其相关基因。 还描述了在其中整合这些基因的载体，用所述载体转化的重组微生物，以及使用所述重组微生物制备杏仁N-糖苷酶的方法。

公开(公告)号：US20080044903A1

公开(公告)日：2008-02-21

申请号：US11829313

申请日：2007-07-27

申请人： Mitsuhiro UENO ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

发明人： Mitsuhiro UENO ,  Hirofumi Yoshioka ,  Haruko Konishi ,  Kimikazu Hashino ,  Mio Morishita ,  Hideto Chono ,  Tsuyoshi Miyamura ,  Mutsumi Sano ,  Kiyozo Asada ,  Kei Fujinaga ,  Ikunoshin Kato

IPC分类号： C12N5/02

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/10 ,  C12N2810/80 ,  C12N2810/851 ,  C12N2810/859

摘要： Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferring receptor, or introducing a new functional group into the functional substance.

摘要翻译： 通过使用逆转录病毒将基因转移到靶细胞中的改进方法，其中基因转移效率得到改善，并且通过将逆转录病毒结合到固定在载体上并具有结合逆转录病毒活性的功能物质来有效转化靶细胞 随后洗涤; 使用能够特异性识别细胞的抗体，层粘连蛋白或富甘糖型糖链作为具有与靶细胞结合的活性的物质; 预处理靶细胞以使转移受体失活，或将新的官能团引入功能物质。