摘要:
DNA probes for detecting the presence of Helicobacter pylori are provided. These isolated, purified oligonucleotide probes are useful in assays for the specific detection of Helicobacter pylori because they specifically hybridize to the DNA of H. pylori.
摘要:
A digested phagocyte prepared by contacting in vitro a phagocyte with a foreign microorganism and isolating the phagocyte so contacted; a process for producing the same; and a process and a kit in which these are utilized are disclosed. An experimental model, which enables in vitro evaluation of a phagocytotic function of phagocytes, is provided.
摘要:
A probe derived from bacteria of pneumonia, containing fragments of DNA which streptococcus pneumoniae essentially possesses, and useful for detecting and identifying causative bacteria of pneumonia is obtained by completely digesting the DNA with a restriction endonuclease PstI, followed by cloning into a suitable vector.
摘要:
The DNA from the bacteria Bactereoides fragilis is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Bacteroides fragilis, then the sequence of the probe is elucidated.
摘要:
The DNA from the bacteria Klebsiella pneumoniae is extracted, then completely digested with restriction enzyme Hind III, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Klebsiella pneumoniae, then the sequence of the probe is elucidated.
摘要:
The DNA from the bacteria Streptococcus pyogenes is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Streptococcus pyogenes, then the sequence of the probe is elucidated.
摘要:
The invention provides a probe useful for rapidly detecting and identifying fungi causative of infectious diseases by digesting completely a DNA extracted from a fungus, e.g., Candida albicans, with EcoRI, inserting the same into an appropriate cloning vector, and selecting a probe containing DNA fragments unique to each fungus. The invention also provides a standard sequence suitable for making reference to a genomic DNA contained in a clinical specimen by elucidating the base sequence of the probe thus selected.
摘要:
Disclosed are anti-HIV agents which comprise a mannose binding protein (MBP) as an active component and are useful for effectively inhibiting progress of diseases state in and useful in therapy for individuals infected with human immunodeficiency virus (HIV). Also disclosed are a method for evaluating an anti-HIV activity of MBP comprising the step of culturing HIV infected cells under the presence of MBP.
摘要:
An objective of the present invention is to provide methods of producing human collagen molecules that are easy to isolate and purify and that have a structure substantially equivalent to that of a natural collagen molecule, wherein host cells that are transduced with a collagen gene synthesize large amounts of human collagen protein derived from a gene introduced into a high exogenous gene expression vector. Another objective of the present invention is to provide collagen molecules produced by the production methods.The present inventors discovered that a large amount of human collagen hardly contaminated with host cell-derived collagen could be produced, by selecting from various mammalian cells a host cell that has low collagen expression and introducing a collagen gene construct into a vector capable of high exogenous gene expression.
摘要:
Provided are isolated collectin (CL-L2s) genes including a base sequence set out in SEQ ID NO: 1, 3, 5, 7, 9, 12, 36, 38 or 40 relating to a novel collectin which are expected to exhibit an antibacterial activity, an antiviral activity and the like particularly in a human body; and isolated collectin proteins including an amino acid sequence set out in SEQ ID NO: 2, 4, 6, 8, 10, 13, 37, 39 or 41 and derivatives and fragments thereof.
摘要翻译:提供了分离的集合蛋白(CL-L2)基因,其包括与预期显示抗细菌剂的新型聚集蛋白相关的SEQ ID NO:1,3,5,7,9,12,32,38或40所示的碱基序列 活动,抗病毒活性等,特别是在人体内; 和分离的集合蛋白,包括SEQ ID NO:2,4,6,8,10,13,37,39或41所示的氨基酸序列及其衍生物和片段。