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公开(公告)号：US07745182B2
公开(公告)日：2010-06-29
申请号：US12345922
申请日：2008-12-30
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
摘要翻译： 提供了一种生产β-羟基氨基酸及其光学活性异构体的方法。 β-羟基氨基酸通过使预定的D-α-氨基酸和5,10-亚甲基四氢叶酸在衍生自属于副球菌属，氨基杆菌属或Ensifer属的微生物的酶存在下反应来制备。
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公开(公告)号：US07556947B2
公开(公告)日：2009-07-07
申请号：US12345887
申请日：2008-12-30
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
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3.发明申请Recombinant DNA having hydantoinase gene and carbamylase gene and process for producing amino acid 审中-公开具有乙内酰脲酶基因和氨基甲酰酶基因的重组DNA及其生产氨基酸的方法公开(公告)号：US20050112729A1
公开(公告)日：2005-05-26
申请号：US10961116
申请日：2004-10-12
摘要： The object of the present invention is to prepare a recombinant DNA efficiently co-expressing genes of enzymes such as HHase, and to produce an amino acid efficiently from hydantoin by utilizing such a recombinant DNA. The present invention provides a recombinant DNA having base sequences encoding the following gene regions (I) and (II) incorporated therein in a predetermined direction and in a predetermined order to produce an amino acid efficiently: (I) a gene region containing a base sequence encoding a hydantoinase gene and a trp promoter sequence located upstream of the base sequence encoding the hydantoinase gene regulating expression of the hydantoinase gene, and (II) a gene region containing a base sequence encoding a carbamylase gene and a trp promoter sequence located upstream of the base sequence encoding the carbamylase gene regulating expression of the carbamylase gene.
摘要翻译： 本发明的目的是制备有效共同表达诸如HHase的酶的基因的重组DNA，并通过利用这种重组DNA从乙内酰脲有效地产生氨基酸。 本发明提供一种具有编码下述基因区（I）和（II）的碱基序列的重组DNA，其特征在于，在规定的方向和规定的顺序中并入其中，以有效地产生氨基酸：（I）含有碱基序列 编码乙内酰脲酶基因和位于编码修饰乙内酰脲酶基因表达的乙内酰脲酶基因的碱基序列上游的trp启动子序列，以及（II）含有编码氨基甲酰化酶基因的碱基序列和位于上游的trp启动子序列的基因区域 编码调节氨基甲酰酶基因表达的氨基甲酰酶基因的碱基序列。
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公开(公告)号：US20060263861A1
公开(公告)日：2006-11-23
申请号：US11419522
申请日：2006-05-22
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
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5.发明申请Novel aldolase, and method for producing optically active IHOG and monatin 失效新型醛缩酶，以及用于制备光学活性的IHOG和莫纳甜的方法公开(公告)号：US20060172396A1
公开(公告)日：2006-08-03
申请号：US11362915
申请日：2006-02-28
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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6.发明授权公开(公告)号：US08048647B2
公开(公告)日：2011-11-01
申请号：US12635994
申请日：2009-12-11
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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公开(公告)号：US20090170171A1
公开(公告)日：2009-07-02
申请号：US11942873
申请日：2007-11-20
CPC分类号： C12N9/1014 , C12P13/06 , C12Y201/02001
摘要： The present invention provides a new method for producing serine derivatives and their optically-activated derivatives in a convenient manner. In the presence of an enzyme, an L-α-amino acid of formula (I): (in the formula (I), R1 is a hydrocarbon group) is reacted with an aldehyde of formula (II): (in the formula (II), R2 is a hydrocarbon) to produce an L-serine derivative of formula (III).
摘要翻译： 本发明提供了以方便的方式制备丝氨酸衍生物及其光活性衍生物的新方法。 在酶的存在下，式（I）:(式（I）中的R 1为烃基）的式（I）的L-α-氨基酸与式（II）的醛反应：（在式 II），R 2为烃），得到式（III）的L-丝氨酸衍生物。
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8.发明授权公开(公告)号：US07371549B2
公开(公告)日：2008-05-13
申请号：US11362915
申请日：2006-02-28
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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9.发明授权公开(公告)号：US07241599B2
公开(公告)日：2007-07-10
申请号：US11146093
申请日：2005-06-07
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译： 本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。 本发明还涉及用于制备光学活性莫纳甜的方法和用于这些方法的醛缩酶。 因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体 酸和丙酮酸（或草酰乙酸）。
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公开(公告)号：US20050214912A1
公开(公告)日：2005-09-29
申请号：US11091892
申请日：2005-03-29
申请人： Hiroyuki Nozaki , Kunihiko Watanabe
发明人： Hiroyuki Nozaki , Kunihiko Watanabe
CPC分类号： C12P13/225
摘要： A method for producing an optically active amino acid comprising contacting a 5-substituted hydantoin with a group of enzymes including both a hydantoinase and a carbamoylase, wherein the method is carried out in an aqueous solution with a dissolved oxygen concentration of 1.5 ppm or less. Optically active amino acids such as D-tyrosine may be produced with high efficiency.
摘要翻译： 一种光学活性氨基酸的制造方法，其特征在于，将5-取代的乙内酰脲与包括乙内酰脲酶和氨甲酰基酶的酶组合，其中所述方法在溶解氧浓度为1.5ppm以下的水溶液中进行。 可以高效率地生产D-酪氨酸等光活性氨基酸。
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