公开(公告)号：US08623608B2

公开(公告)日：2014-01-07

申请号：US13374807

申请日：2012-01-13

申请人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

发明人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： G01N33/68 ,  C07K14/4726 ,  C07K14/473 ,  G01N33/5308 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2333/4724 ,  G01N2333/4728 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2800/085 ,  Y10T436/143333

摘要： An object of the present invention is to provide a method for measuring a glycan-marker glycoprotein, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a method for examining liver disease, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Disclosed is a method for measuring at least one glycoprotein selected from alpha-1-acid glycoprotein (AGP) and Mac-2-binding protein (M2BP) contained in a sample collected from a subject, comprising: measuring AGP binding to a first lectin selected from AOL and MAL, when the glycoprotein is AGP; and measuring M2BP binding to a second lectin selected from WFA, BPL, AAL, RCA120, and TJAII, when the glycoprotein is M2BP.

摘要翻译： 本发明的目的是提供一种测定聚糖标记糖蛋白的方法，通过该方法可以以比常规方法更高的精度检测肝脏疾病。 另外，本发明的目的在于提供一种检查肝脏疾病的方法，通过该方法能够以比常规方法更高的精度检测肝脏疾病。 公开了一种测量从受试者收集的样品中含有的至少一种选自α-1-酸糖蛋白（AGP）和Mac-2结合蛋白（M2BP）的糖蛋白的方法，包括：测量与所选第一凝集素的AGP结合 来自AOL和MAL，当糖蛋白是AGP时; 并且当糖蛋白是M2BP时，测量M2BP与选自WFA，BPL，AAL，RCA120和TJAII的第二凝集素的结合。

公开(公告)号：US20120172247A1

公开(公告)日：2012-07-05

申请号：US13374807

申请日：2012-01-13

申请人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

发明人： Hisashi Narimatsu ,  Yuzuru Ikehara ,  Atsushi Kuno ,  Maki Sogabe ,  Yasuhito Tanaka ,  Masashi Mizokami ,  Kiyoaki Ito ,  Shunsuke Matsubara ,  Chikayuki Tsuruno ,  Youichi Takahama ,  Takashi Kagawa ,  Shinya Nagai

IPC分类号： C40B30/04 ,  G01N33/82 ,  C07K14/47 ,  G01N33/566

CPC分类号： G01N33/68 ,  C07K14/4726 ,  C07K14/473 ,  G01N33/5308 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2333/4724 ,  G01N2333/4728 ,  G01N2400/00 ,  G01N2400/02 ,  G01N2800/085 ,  Y10T436/143333

摘要： An object of the present invention is to provide a method for measuring a glycan-marker glycoprotein, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Also, an object of the present invention is to provide a method for examining liver disease, by which liver disease can be detected with higher accuracy than is possible with conventional methods. Disclosed is a method for measuring at least one glycoprotein selected from alpha-1-acid glycoprotein (AGP) and Mac-2-binding protein (M2BP) contained in a sample collected from a subject, comprising: measuring AGP binding to a first lectin selected from AOL and MAL, when the glycoprotein is AGP; and measuring M2BP binding to a second lectin selected from WFA, BPL, AAL, RCA120, and TJAII, when the glycoprotein is M2BP.

摘要翻译： 本发明的目的是提供一种测定聚糖标记糖蛋白的方法，通过该方法可以以比常规方法更高的精度检测肝脏疾病。 另外，本发明的目的在于提供一种检查肝脏疾病的方法，通过该方法能够以比常规方法更高的精度检测肝脏疾病。 公开了一种测量从受试者收集的样品中含有的至少一种选自α-1-酸糖蛋白（AGP）和Mac-2结合蛋白（M2BP）的糖蛋白的方法，包括：测量与所选第一凝集素的AGP结合 来自AOL和MAL，当糖蛋白是AGP时; 并且当糖蛋白是M2BP时，测量M2BP与选自WFA，BPL，AAL，RCA120和TJAII的第二凝集素的结合。

公开(公告)号：US09796761B2

公开(公告)日：2017-10-24

申请号：US13384031

申请日：2010-07-12

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C40B30/04 ,  C07K14/00 ,  C07K9/00 ,  C07K14/47 ,  G01N33/574

CPC分类号： C07K9/00 ,  C07K14/47 ,  G01N33/57438 ,  G01N33/57469 ,  G01N2400/02 ,  G01N2800/085

摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.

公开(公告)号：US20120190576A1

公开(公告)日：2012-07-26

申请号：US13384031

申请日：2010-07-12

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C40B30/04 ,  C07K14/00

CPC分类号： C07K9/00 ,  C07K14/47 ,  G01N33/57438 ,  G01N33/57469 ,  G01N2400/02 ,  G01N2800/085

摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.

摘要翻译： 本发明涉及开发能够检测肝脏疾病的聚糖标记物，更具体地说，涉及发展表达肝病状态的聚糖标记物。 此外，本发明还涉及开发能够区分肝脏疾病状态与肝癌进展的聚糖标记。 本发明人鉴定了血清糖蛋白中由于包括肝癌在内的肝脏疾病而特异性地改变聚糖结构的糖肽和糖蛋白，并将其提供为肝脏疾病状态特异性的新型聚糖标记（糖肽和糖蛋白）。

公开(公告)号：US20150293104A1

公开(公告)日：2015-10-15

申请号：US14413846

申请日：2012-07-12

申请人： Hirofumi Nozaki ,  Takashi Ohkura ,  Atsushi Kuno ,  Maki Sogabe ,  Tomomi Kubota ,  Hiroyuki Kaji ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Hirofumi Nozaki ,  Takashi Ohkura ,  Atsushi Kuno ,  Maki Sogabe ,  Tomomi Kubota ,  Hiroyuki Kaji ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/574

CPC分类号： G01N33/57449 ,  C07K14/47 ,  G01N33/6842 ,  G01N2333/78 ,  G01N2333/8121 ,  G01N2333/902 ,  G01N2333/90287 ,  G01N2333/96458

摘要： An object of the present invention is to develop and provide an epithelial ovarian cancer diagnosis marker with which epithelial ovarian cancer can be detected inexpensively, conveniently, and low invasively with high accuracy, and a method for determining the presence or absence of epithelial ovarian cancer using the marker. The present invention provides a glycoprotein having a glycan-linked asparagine residue at a particular site of the glycoprotein secreted from an epithelial ovarian cancer cell, or a fragment thereof having the glycan as an epithelial ovarian cancer diagnosis marker. The present invention also provides a method for determining the presence or absence of epithelial ovarian cancer using the glycoprotein.

摘要翻译： 本发明的目的是开发和提供一种上皮性卵巢癌诊断标记，其可以廉价地，方便地和低侵入性高精度地检测上皮性卵巢癌，以及使用以下方法确定上皮性卵巢癌的存在或不存在的方法： 标记。 本发明提供一种在从上皮性卵巢癌细胞分泌的糖蛋白的特定部位或其具有聚糖作为上皮性卵巢癌诊断标记的片段的具有聚糖连接的天冬酰胺残基的糖蛋白。 本发明还提供了使用糖蛋白来确定上皮性卵巢癌的存在或不存在的方法。

公开(公告)号：US10247733B2

公开(公告)日：2019-04-02

申请号：US14234871

申请日：2012-07-26

申请人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/50 ,  G01N33/574 ,  C07K16/40

摘要： It is intended to find a highly specific epithelial ovarian cancer marker and to provide an antibody capable of specifically recognizing and detecting the marker or a fragment of the antibody. The present invention provides an anti-β1,6-N-acetylglucosaminyltransferase 5B antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,6-N-acetylglucosaminyltransferase 5B as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

公开(公告)号：US09816996B2

公开(公告)日：2017-11-14

申请号：US14234783

申请日：2012-07-26

申请人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/574 ,  C12N5/16 ,  C07K16/30 ,  C07K16/40

CPC分类号： G01N33/57449 ,  C07K16/3069 ,  C07K16/40 ,  C12N5/163

摘要： The present invention provides an antibody capable of specifically recognizing and detecting the highly specific cancer marker with respect to the epithelial ovarian cancer, or a fragment of the antibody. The present invention provides an anti-β1,3-N-acetylglucosaminyltransferase 3 antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,3-N-acetylglucosaminyltransferase 3 as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

公开(公告)号：US20140242607A1

公开(公告)日：2014-08-28

申请号：US14234871

申请日：2012-07-26

申请人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/574 ,  C07K16/40

CPC分类号： G01N33/57449 ,  C07K16/40 ,  C07K2317/33 ,  C07K2317/34

摘要： It is intended to find a highly specific epithelial ovarian cancer marker and to provide an antibody capable of specifically recognizing and detecting the marker or a fragment of the antibody. The present invention provides an anti-β1,6-N-acetylglucosaminyltransferase 5B antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,6-N-acetylglucosaminyltransferase 5B as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

摘要翻译： 旨在找到高度特异性的上皮性卵巢癌标记物，并提供能够特异性识别和检测抗体的标记或片段的抗体。 本发明提供了一种用于诊断上皮性卵巢癌的抗 - 1gr-1-1-Gly-Gly-Gly-glucogaminamintransferase 5B抗体，即用于检测作为上皮性卵巢癌标志物的糖基转移酶和1,6-N-乙酰葡糖胺基转移酶5B的抗体 。 抗体识别由SEQ ID NO：1表示的氨基酸序列组成的酶的多肽的一部分作为表位。

公开(公告)号：US20140295455A1

公开(公告)日：2014-10-02

申请号：US14234783

申请日：2012-07-26

申请人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/574 ,  C07K16/40

CPC分类号： G01N33/57449 ,  C07K16/3069 ,  C07K16/40 ,  C12N5/163

摘要： The present invention provides an antibody capable of specifically recognizing and detecting the highly specific cancer marker with respect to the epithelial ovarian cancer, or a fragment of the antibody. The present invention provides an anti-β1,3-N-acetylglucosaminyltransferase 3 antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,3-N-acetylglucosaminyltransferase 3 as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

摘要翻译： 本发明提供能够特异性识别和检测关于上皮性卵巢癌或抗体片段的高度特异性癌症标志物的抗体。 本发明提供了用于诊断上皮性卵巢癌的抗 - 1,3-N-乙酰氨基葡糖转移酶3抗体，即用于检测糖基转移酶的抗体和作为上皮性卵巢癌标志物的1,3-N-乙酰氨基葡糖转移酶3 。 抗体识别由SEQ ID NO：1表示的氨基酸序列组成的酶的多肽的一部分作为表位。

公开(公告)号：US20140323324A1

公开(公告)日：2014-10-30

申请号：US13994435

申请日：2011-12-16

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

IPC分类号： G01N33/68

CPC分类号： G01N33/6896 ,  G01N33/6842 ,  G01N2333/47 ,  G01N2333/4728 ,  G01N2400/40 ,  G01N2800/285 ,  H01J49/0036

摘要： The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.

摘要翻译： 本发明的目的是开发：从体液或中枢神经系统细胞中选择性分离衍生自中枢神经系统的糖蛋白的方法; 以及利用上述方法搜索中枢神经系统疾病指标的方法。 可以通过两步分离方法选择性地富集来自中枢神经系统的蛋白质，其在体液或中枢神经系统细胞中发生微量的蛋白质，其包括在非还原末端除去具有唾液酸的糖蛋白 从体液或中枢神经系统细胞中分离，然后在其非还原末端分离具有N-乙酰葡糖胺的糖蛋白。