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    Autoradiographic gene-screening method
    1.
    发明授权
    Autoradiographic gene-screening method 失效
    放射自显影基因筛选方法

    公开(公告)号：US5270162A

    公开(公告)日：1993-12-14

    申请号：US735675

    申请日：1991-07-29

    申请人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    发明人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N27/26 ,  G03C5/16

    CPC分类号： C12Q1/6825

    摘要： An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring at least a portion of nucleic acids, fragments thereof or derivatives thereof resolved on a medium onto a transfer support to fix them thereonto;(2) a step of hybridizing the nucleic acids, fragments thereof or derivatives thereof fixed onto said transfer support with radioactively labeled probes; and(3) a step of obtaining locational information on the radioactively labeled substances on said transfer support, which comprises placing said transfer support having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on said transfer support, exciting said stimulable phosphor sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission.

    摘要翻译： 使用杂交方法的放射自显影基因筛选方法，其包括：（1）将至少一部分核酸，其片段或衍生物在培养基上转移到转移载体上以将其固定在其上的步骤; （2）使用放射性标记的探针将固定在所述转移载体上的核酸，其片段或其衍生物杂交的步骤; （3）获得所述转移支持体上的放射性标记物质的位置信息的步骤，其包括将经过杂交的所述转移支持体和可刺激的荧光体片层层叠一段给定的时间，以使所述片材 吸收由所述转印支撑体上的放射性标记物质发射的辐射能量的至少一部分，用电磁波激发所述可刺激的荧光粉片，以释放作为受激发射存储在所述片材中的辐射能量，以及检测受激发射。

    Autoradiographic gene-screening method
    2.
    发明授权
    Autoradiographic gene-screening method 失效
    放射自显影基因筛选方法

    公开(公告)号：US4865967A

    公开(公告)日：1989-09-12

    申请号：US127605

    申请日：1987-11-30

    申请人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    发明人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58 ,  G01N33/60 ,  G01T1/29

    CPC分类号： G01T1/2942 ,  C12Q1/6841

    摘要： An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring a portion of genetic clones cultured on a culture medium onto a filter to fix them thereonto;(2) a step of hybridizing the genes of said clones fixed onto said filter with radioactively labeled probes;(3) a step of obtaining two dimensional information on the location of the radioactively labeled substances on the filter which comprises placing said filter having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said stimulable phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on the filter, exciting said sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission to obtain a locational information on the radioactively labeled substances on the filter; and(4) a step recovering the clones on the culture medium according to the obtained locational information.

    摘要翻译： 使用杂交方法的放射自显影基因筛选方法，其包括：（1）将在培养基上培养的一部分遗传克隆转移到过滤器上以将其固定在其上的步骤; （2）将固定在所述过滤器上的所述克隆的基因与放射性标记的探针杂交的步骤; （3）获得关于过滤器上的放射性标记物质的位置的二维信息的步骤，该步骤包括将已经进行了杂交的所述过滤器和可刺激的磷光体片层压一段给定的时间以引起所述可刺激的荧光体 以吸收由过滤器上的放射性标记的物质发射的辐射能量的至少一部分，用电磁波激发所述片材，以释放作为受激发射存储在所述片材中的辐射能量，并且检测受激发射以获得位置信息 在过滤器上的放射性标记物质上; 和（4）根据获得的位置信息恢复培养基上的克隆的步骤。

    Stimulable phosphor sheet with hydrophilic surface
    3.
    发明授权
    Stimulable phosphor sheet with hydrophilic surface 失效
    具有亲水性表面的稳定的荧光粉片

    公开(公告)号：US4617468A

    公开(公告)日：1986-10-14

    申请号：US582767

    申请日：1984-02-23

    申请人： Hisashi Shiraishi ,  Hisatoyo Kato ,  Junji Miyahara

    发明人： Hisashi Shiraishi ,  Hisatoyo Kato ,  Junji Miyahara

    IPC分类号： G01T1/29 ,  G21K4/00 ,  G01T1/10

    CPC分类号： G21K4/00 ,  G01T1/2942

    摘要： A stimulable phosphor sheet preferably employable in autoradiography which comprises a substrate, a phosphor layer being provided on the substrate and comprising a stimulable phosphor dispersed in a binder, and a protective layer provided on the phosphor layer, characterized in that the surface of the protective layer is hydrophilic.The surface of the protective layer can be made hydrophilic by hydrophilic processing applied thereto or provision of a hydrophilic layer thereon.

    摘要翻译： 优选可用于放射自显影的可激发的荧光体片，其包括基底，荧光体层设置在所述基底上，并且包含分散在粘合剂中的可刺激的荧光体，以及设置在所述荧光体层上的保护层，其特征在于，所述保护层 是亲水的。 保护层的表面可以通过亲水处理施加到其上或在其上提供亲水层而使其亲水。

    Autoradiographic process
    4.
    发明授权
    Autoradiographic process 失效
    放射自显影过程

    公开(公告)号：US5260190A

    公开(公告)日：1993-11-09

    申请号：US378509

    申请日：1989-07-06

    申请人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    发明人： Hisashi Shiraishi ,  Junji Miyahara ,  Hisatoyo Kato

    IPC分类号： G01T1/29 ,  C12Q1/68 ,  G01N33/566 ,  G03C5/16

    CPC分类号： G01T1/2014 ,  G01T1/2012 ,  Y10T436/143333

    摘要： An autoradiographic process for obtaining information on one or two dimensional location of radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances such as DNA originating from an organism,which comprises:placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers for a certain period to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;scanning said phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet as stimulated emission; anddetecting the stimulated emission to obtain locational information on the radioactively labeled substances in the sample.

    摘要翻译： 一种放射自显影方法，用于获得选自以下的样品中所含的放射性标记物质的一维或二维位置的信息：生物体组织和含生物体组织的培养基和/或源自生物的DNA的物质， 其包括：将所述样品和可激发的荧光体片材具有包含分散在粘合剂中的可刺激磷光体的荧光体层一起分层一定时间，以使所述荧光体片材吸收由放射性标记的物质发射的辐射能量的至少一部分 在所述样品中; 用电磁波扫描所述荧光粉片，以释放存储在所述荧光粉片中的辐射能量的至少一部分作为受激发射; 并检测受激发射以获得样品中放射性标记物质的位置信息。

    Signal processing method in autoradiography
    6.
    发明授权
    Signal processing method in autoradiography 失效
    放射自显影中的信号处理方法

    公开(公告)号：US4852050A

    公开(公告)日：1989-07-25

    申请号：US568907

    申请日：1984-01-06

    申请人： Hisashi Shiraishi ,  Tsutomu Kimura ,  Kazuhiro Hishinuma

    发明人： Hisashi Shiraishi ,  Tsutomu Kimura ,  Kazuhiro Hishinuma

    IPC分类号： G01T1/29

    CPC分类号： G01T1/2014 ,  G01T1/2012 ,  Y10S250/909

    摘要： A signal processing method in autoradiography for determination of the base sequence of DNA or a DNA fragment, employing groups of radioactively labeled base specific cleavage products or mixtures thereof obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element and resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium. An autograph is obtained having locational information on groups of radioactively labeled cleavage products contained in the rows on the support medium. An electrical digital signal corresponding to the autoradiograph is then generated by recording and reproducing from a stimulable phosphor sheet the radiation energy stored therein. Sampling points in each resolved row of the digital signal are next detected. Reference sampling points are then determined in a plurality of reference rows which are either directly provided on the support medium and/or synthesized from certain resolved rows. Sampling points in the remaining non-reference rows are identified by comparison with the continuous lines to thus determine the base sequence of the DNA or DNA fragment.

    摘要翻译： 放射自显影法中的信号处理方法，用于测定DNA或DNA片段的碱基序列，采用放射性标记的碱基特异性切割产物组或其混合物，其通过特异性切割用放射性元素标记的DNA或DNA片段并一维拆分 彼此平行地在支撑介质上形成分辨行。 获得具有位于支持介质上的行中包含的放射性标记的裂解产物组的位置信息的签名。 然后通过从可刺激的荧光体片材记录和再现其中存储的辐射能量来生成对应于放射自显影仪的电数字信号。 接下来检测数字信号的每个解决行中的采样点。 然后在多个参考行中确定参考采样点，这些参考行直接设置在支持介质上和/或从某些已解决的行合成。 通过与连续线进行比较来确定剩余非参考行中的采样点，从而确定DNA或DNA片段的碱基序列。

    Signal detecting method in autoradiography
    7.
    发明授权
    Signal detecting method in autoradiography 失效
    放射自显影中的信号检测方法

    公开(公告)号：US4665312A

    公开(公告)日：1987-05-12

    申请号：US773858

    申请日：1985-09-09

    申请人： Hisashi Shiraishi ,  Tsutomu Kimura ,  Kazuhiro Hishinuma

    发明人： Hisashi Shiraishi ,  Tsutomu Kimura ,  Kazuhiro Hishinuma

    IPC分类号： G03B42/02 ,  G01N27/26 ,  G01N27/447 ,  G01N33/50 ,  G01T1/29 ,  G01T1/20

    CPC分类号： G01T1/2942 ,  G01T1/2014

    摘要： A signal detecting method in autoradiography, which is applied to an autoradiography having locational information on radioactively labeled substances such as radioactively labeled biopolymers distributed one-dimensionally, being recorded on a stimulable phosphor sheet as a radiation energy-stored image, which comprises steps of:(1) preliminarily scanning a part of the stimulable phosphor sheet with an electromagnetic wave to release a portion of the radiation energy as stimulated emission and detecting the stimulated emission to give an electric signal, to which a signal processing is applied, to determine one-dimensional distribution direction of the radioactively labeled substances; and(2) finally scanning the stimulable phosphor sheet with an electromagnetic wave along said one-dimensional distribution direction to release the radiation energy as stimulated emission and detecting the stimulated emission, to obtain the locational information on the radioactively labeled substances as a digital signal.

    摘要翻译： 一种放射自显影中的信号检测方法，其应用于具有放射性标记物质的位置信息的放射自显影方法，所述放射自显影物质例如放射性标记的一维分布的生物聚合物作为辐射能量存储图像记录在可刺激的磷光体片上，其包括以下步骤： （1）用电磁波对一部分可刺激的荧光体片进行扫描，以释放辐射能量的一部分作为受激发射，并检测受激发射，给出施加信号处理的电信号， 放射性标记物质的尺寸分布方向; 和（2）最后用沿着所述一维分布方向的电磁波扫描所述可刺激的荧光体片材，以释放作为受激发射的辐射能量并检测受激发射，以获得关于放射性标记物质的位置信息作为数字信号。