公开(公告)号：US10830763B2

公开(公告)日：2020-11-10

申请号：US15565959

申请日：2016-01-05

Applicant: Hitachi High-Technologies Corporation

Inventor： Ryosuke Takahashi ,  Katsuhiro Kanda ,  Yusuke Shimizu

IPC: G01N33/50 ,  C12M1/00 ,  C12M1/32 ,  C12M1/34 ,  C12M3/00

Abstract: Provided is a component analysis device, which comprises an analysis unit for measuring a component fed to the plurality of containers and analyzing the component thus measured, wherein the plurality of containers are at least a first container and a second container, wherein the first container retains a first solution containing a substance that releases adhesion between multiple cells forming the liver cell tissue, and the second container retains a buffer solution, and wherein the analysis unit measures an amount of the component discharged from the liver cell tissue in the first container into the first solution and an amount of the component discharged from the liver cell tissue in the second container into the buffer solution in the second container, and analyzes an amount of the component to be discharged via a bile duct in the liver cell tissue.

公开(公告)号：US10684276B2

公开(公告)日：2020-06-16

申请号：US15847092

申请日：2017-12-19

Applicant: Hitachi High-Technologies Corporation

Inventor： Ryosuke Takahashi ,  Katsuhiro Kanda ,  Yusuke Shimizu ,  Hisashi Ichikawa

IPC: G01N33/50 ,  G01N35/10 ,  C12M1/34 ,  B01L7/00 ,  G01N33/15 ,  C12M1/12 ,  C12M1/00 ,  G01N35/00 ,  G01N35/04 ,  B01L3/00 ,  B01L9/00

Abstract: Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

公开(公告)号：US09880153B2

公开(公告)日：2018-01-30

申请号：US14914278

申请日：2014-11-26

Applicant: Hitachi High-Technologies Corporation

Inventor： Ryosuke Takahashi ,  Katsuhiro Kanda ,  Yusuke Shimizu ,  Hisashi Ichikawa

IPC: C12M1/34 ,  G01N33/50 ,  G01N33/15 ,  C12M1/12 ,  C12M1/00 ,  G01N35/00 ,  G01N35/04

CPC classification number: G01N33/5067 ,  B01L3/5085 ,  C12M25/00 ,  C12M29/00 ,  C12M41/46 ,  C12M47/20 ,  G01N33/15 ,  G01N33/502 ,  G01N2035/00425 ,  G01N2035/0418

Abstract: Application of the present invention enables quantification of fractions of candidate pharmaceutical compounds (a parent compound and its metabolites), one excreted to the basolateral (Basal/Basolateral)-side via transporters and by diffusion, one excreted to the lumen (Apical)-side, and one remained in the cells. This enables determination of the total amount of the administered candidate pharmaceutical compounds and the distribution ratio of the fractions. The kinetics of the administered candidate pharmaceutical compounds can be evaluated, thereby enabling in vitro screening of an enormous number of candidate pharmaceutical compounds for drug candidates exhibiting the efficacy. The object of the present invention is to provide an apparatus and method for understanding a total picture of pharmacokinetics in vitro by quantifying a fraction of basolateral (Basal/Basolateral) efflux, a fraction of lumen (Apical)-side excretion, and a fraction remaining in a cell of a drug which has been administered to the cell to determine the distribution ratio of each fraction.

公开(公告)号：US08604425B2

公开(公告)日：2013-12-10

申请号：US13680630

申请日：2012-11-19

Applicant: Hitachi High-Technologies Corporation

Inventor： Katsuhiro Kanda ,  Makoto Nogami ,  Izumi Waki

IPC: G01N1/10 ,  G01N1/28 ,  G01N1/02 ,  G01N33/48 ,  H01J49/26

CPC classification number: G01N1/4077 ,  G01N1/405 ,  G01N30/82 ,  G01N2030/009 ,  H01J49/0431 ,  H01J49/26

Abstract: The present invention relates to a pretreatment apparatus that performs concentration and separation of a sample, and in particular, in order to provide a sample pretreatment apparatus using a solid-phase extraction column, and a mass spectrometer using the same, which is particularly suitable for clinical analysis in which qualitative/quantitative analysis of a biological sample such as blood is performed, according to each operational step for a solid-phase extraction treatment, for example, a collection device serving as flow passages or containers for collection of waste liquid or extracted matter is installed on a bottom face of the solid-phase extraction column, and the extracted matter is separately collected without being mixed with waste liquid by switching the positions of the collection device.

Abstract translation: 本发明涉及一种进行样品浓缩分离的预处理装置，特别是为了提供使用固相萃取柱的样品预处理装置和使用其的质谱仪，特别适用于 根据固相萃取处理的每个操作步骤，例如用作流动通道的收集装置或用于收集废液或提取的容器的容器，进行诸如血液的生物样品的定性/定量分析的临床分析 物质被安装在固相萃取塔的底面上，并且通过切换收集装置的位置而分离地收集提取物而不与废液混合。