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公开(公告)号：US08951302B2
公开(公告)日：2015-02-10
申请号：US12576826
申请日：2009-10-09
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
摘要翻译： 本发明提供了制造包括流体网络的支架的方法，包括以下步骤：（a）产生用于封闭腔室并向细胞提供流体的初始血管层，初始血管层具有用于流体的通道网络; （b）将初始血管层转化为用于流体动力学分析的模型; （c）基于从由特定流体的特性，输入压力，输出压力，总体流速及其组合组成的组中选择的期望参数来分析初始血管层，以确定网络内的纯粹的应力和速度 渠道; （d）测量纯应力和速度，并将获得的值与预定值进行比较; （e）确定剪切应力或速度是否大于或小于预定值，和（f）任选地修饰初始血管层并重复步骤（b） - （e）。 本发明还提供包含用于组织层的血管层的组合物以及具有血管层和试剂盒的医疗装置。
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公开(公告)号：US08591597B2
公开(公告)日：2013-11-26
申请号：US12728557
申请日：2010-03-22
IPC分类号： A61F2/04
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
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公开(公告)号：US10327885B2
公开(公告)日：2019-06-25
申请号：US14615646
申请日：2015-02-06
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
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公开(公告)号：US20100234678A1
公开(公告)日：2010-09-16
申请号：US12576826
申请日：2009-10-09
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
摘要翻译： 本发明提供了制造包括流体网络的支架的方法，包括以下步骤：（a）产生用于封闭腔室并向细胞提供流体的初始血管层，初始血管层具有用于流体的通道网络; （b）将初始血管层转化为用于流体动力学分析的模型; （c）基于从由特定流体的特性，输入压力，输出压力，总体流速及其组合组成的组中选择的期望参数来分析初始血管层，以确定网络内的纯粹的应力和速度 渠道; （d）测量纯应力和速度，并将获得的值与预定值进行比较; （e）确定剪切应力或速度是否大于或小于预定值，和（f）任选地修饰初始血管层并重复步骤（b） - （e）。 本发明还提供包含用于组织层的血管层的组合物以及具有血管层和试剂盒的医疗装置。
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公开(公告)号：US20150366651A1
公开(公告)日：2015-12-24
申请号：US14615646
申请日：2015-02-06
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
摘要翻译： 本发明提供了制造包括流体网络的支架的方法，包括以下步骤：（a）产生用于封闭腔室并向细胞提供流体的初始血管层，初始血管层具有用于流体的通道网络; （b）将初始血管层转化为用于流体动力学分析的模型; （c）基于从由特定流体的特性，输入压力，输出压力，总体流速及其组合组成的组中选择的期望参数来分析初始血管层，以确定网络内的纯粹的应力和速度 渠道; （d）测量纯应力和速度，并将获得的值与预定值进行比较; （e）确定剪切应力或速度是否大于或小于预定值，和（f）任选地修饰初始血管层并重复步骤（b） - （e）。 本发明还提供包含用于组织层的血管层的组合物以及具有血管层和试剂盒的医疗装置。
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公开(公告)号：US20100274353A1
公开(公告)日：2010-10-28
申请号：US12728557
申请日：2010-03-22
IPC分类号： A61F2/06
CPC分类号： A61F2/06 , A61F2/022 , A61F2/04 , A61F2/062 , A61F2002/068 , G01N33/5008 , G06F19/00
摘要： The invention provides method of fabricating a scaffold comprising a fluidic network, including the steps of: (a) generating an initial vascular layer for enclosing the chamber and providing fluid to the cells, the initial vascular layer having a network of channels for fluid; (b) translating the initial vascular layer into a model for fluid dynamics analysis; (c) analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid, an input pressure, an output pressure, an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels; (d) measuring the sheer stress and the velocity and comparing the obtained values to predetermined values; (e) determining if either of the shear stress or the velocity are greater than or less than the predetermined values, and (f) optionally modifying the initial vascular layer and repeating steps (b)-(e). The invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits.
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公开(公告)号：US20110091930A1
公开(公告)日：2011-04-21
申请号：US12866970
申请日：2009-02-13
CPC分类号： G01N33/5014 , C12M25/04 , C12M35/08 , G01N33/5067
摘要： A well-based flow system for cell culture is described which provides for flow of culture containing compounds for drug screening to be exposed to cells seeded on a membrane. The flow of medium may be planar or radial and means are provided for the removal of waste media through fluid outlets in fluid communication with the assay well plates through conduits. Methods of using the system for cell culture and drug toxicity screening are also provided including coculturing cells such as hepatocytes, stem cells, fibroblasts and smooth muscle cells and selectively exposing cells to test compounds.
摘要翻译： 描述了用于细胞培养的基于基础的流动系统，其提供含有用于药物筛选的化合物的培养物流，以暴露于接种在膜上的细胞。 介质的流动可以是平面的或径向的，并且提供了用于通过导管与测定孔板流体连通的流体出口去除废弃介质的装置。 还提供了使用该系统用于细胞培养和药物毒性筛选的方法，包括共培养细胞如肝细胞，干细胞，成纤维细胞和平滑肌细胞，并选择性地将细胞暴露于测试化合物。
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公开(公告)号：US20110195056A1
公开(公告)日：2011-08-11
申请号：US12999238
申请日：2009-07-31
申请人： Howard I. Pryor , Joseph P. Vacanti , David H. Lum , Tim D. Ahfeldt , Chad Cowan
发明人： Howard I. Pryor , Joseph P. Vacanti , David H. Lum , Tim D. Ahfeldt , Chad Cowan
CPC分类号： C12N5/067 , A61K38/00 , C12N5/0667 , C12N2501/115 , C12N2501/12 , C12N2501/60 , C12N2506/1384
摘要： The invention generally features methods for generating hepatocytes from a variety of pluripotent stem cells, including adipose mesenchymal stem cells, therapeutic compositions featuring such cells, and methods of using them for the treatment of subjects.
摘要翻译： 本发明通常具有从多种多能干细胞（包括脂肪间充质干细胞）产生肝细胞的方法，其特征在于这种细胞的治疗组合物，以及用于治疗受试者的方法。
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