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    METHOD OF AND APPARATUS FOR SEPARATING MICROORGANISMS FROM SAMPLE USING ELECTRODIALYSIS AND MICROORGANISM CAPTURING MEANS
    1.
    发明申请
    METHOD OF AND APPARATUS FOR SEPARATING MICROORGANISMS FROM SAMPLE USING ELECTRODIALYSIS AND MICROORGANISM CAPTURING MEANS 审中-公开
    使用电化学和微生物捕获方法从样品中分离微生物的方法和装置

    公开(公告)号：US20080044880A1

    公开(公告)日：2008-02-21

    申请号：US11841191

    申请日：2007-08-20

    申请人： Jung-im HAN ,  Joon-ho KIM ,  Sung-young JEONG ,  Young-sun LEE ,  Kyu-youn HWANG ,  Hun-joo LEE

    发明人： Jung-im HAN ,  Joon-ho KIM ,  Sung-young JEONG ,  Young-sun LEE ,  Kyu-youn HWANG ,  Hun-joo LEE

    IPC分类号： C12N13/00 ,  C12M1/00

    CPC分类号： C12N1/02 ,  B01D61/48

    摘要： A method of separating microorganisms from a sample includes introducing a sample into an apparatus which controls a concentration of at least one salt, the apparatus includes a reaction chamber defined between a cation exchange membrane and an anion exchange membrane, a first electrode chamber defined between the anion exchange membrane and a first electrode, the first electrode chamber containing a first ion exchange medium, a second electrode chamber defined between the cation exchange membrane and a second electrode, the second electrode chamber containing a second ion exchange medium, applying a voltage between the first electrode and the second electrode to electrodialyze the sample in the reaction chamber and reduce the concentration of the at least one salt in the sample and allowing the sample having the reduced concentration of the at least one salt to contact a microorganism capturing means.

    摘要翻译： 从样品中分离微生物的方法包括将样品引入到控制至少一种盐浓度的装置中，该装置包括界定在阳离子交换膜和阴离子交换膜之间的反应室，第一电极室 阴离子交换膜和第一电极，第一电极室包含第一离子交换介质，第二电极室限定在阳离子交换膜和第二电极之间，第二电极室包含第二离子交换介质， 第一电极和第二电极，使反应室中的样品电渗析，并降低样品中至少一种盐的浓度，并允许具有降低的至少一种盐浓度的样品接触微生物捕获装置。

    METHOD OF AMPLIFYING NUCLEIC ACID FROM A CELL USING A NONPLANAR SOLID SUBSTRATE
    2.
    发明申请
    METHOD OF AMPLIFYING NUCLEIC ACID FROM A CELL USING A NONPLANAR SOLID SUBSTRATE 有权
    使用非固体基质从细胞中放大核酸的方法

    公开(公告)号：US20080044864A1

    公开(公告)日：2008-02-21

    申请号：US11841107

    申请日：2007-08-20

    申请人： Sung-young JEONG ,  Kyu-youn HWANG ,  Joon-ho KIM ,  Jung-im HAN ,  Hun-joo LEE

    发明人： Sung-young JEONG ,  Kyu-youn HWANG ,  Joon-ho KIM ,  Jung-im HAN ,  Hun-joo LEE

    IPC分类号： C12P19/34

    CPC分类号： C12N15/1006 ,  B01L7/52 ,  B01L2300/0636 ,  C12N2533/30 ,  C12Q1/6806

    摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

    摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。

    MICROFLUIDIC DEVICE AND METHOD FOR CONCENTRATING SAMPLE CONTAINING CELLS OR VIRUSES AND LYSING CELLS OR VIRUSES, AND METHOD OF PRODUCING THE MICROFLUIDIC DEVICE
    3.
    发明申请
    MICROFLUIDIC DEVICE AND METHOD FOR CONCENTRATING SAMPLE CONTAINING CELLS OR VIRUSES AND LYSING CELLS OR VIRUSES, AND METHOD OF PRODUCING THE MICROFLUIDIC DEVICE 有权
    用于浓缩包含细胞或病毒和感染细胞或病毒的样品的微流体装置和方法以及产生微流体装置的方法

    公开(公告)号：US20070237680A1

    公开(公告)日：2007-10-11

    申请号：US11670002

    申请日：2007-02-01

    申请人： Hun-joo LEE ,  Joon-ho KIM ,  Sung-young JEONG ,  Kyu-youn HWANG

    发明人： Hun-joo LEE ,  Joon-ho KIM ,  Sung-young JEONG ,  Kyu-youn HWANG

    IPC分类号： G01N33/00

    CPC分类号： C12M47/02 ,  C12M47/06

    摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.

    摘要翻译： 一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。 阴极室包括固体支撑体。 微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。

    MICROFLUIDIC DEVICE FOR ELECTROCHEMICALLY REGULATING pH OF FLUID AND METHOD OF REGULATING pH OF FLUID USING THE MICROFLUIDIC DEVICE
    5.
    发明申请
    MICROFLUIDIC DEVICE FOR ELECTROCHEMICALLY REGULATING pH OF FLUID AND METHOD OF REGULATING pH OF FLUID USING THE MICROFLUIDIC DEVICE 有权
    用于电化学调节流体pH的微流体装置和使用微流化装置调节流体pH的方法

    公开(公告)号：US20070138025A1

    公开(公告)日：2007-06-21

    申请号：US11559196

    申请日：2006-11-13

    申请人： Hun-joo LEE ,  Joon-ho KIM ,  Kyu-youn HWANG

    发明人： Hun-joo LEE ,  Joon-ho KIM ,  Kyu-youn HWANG

    IPC分类号： C02F1/461

    CPC分类号： G05D21/02

    摘要： A microfluidic device includes; an ion exchange membrane, an anode chamber one side of which contacts a surface of the ion exchange membrane, wherein the anode chamber further includes a ladder-shaped anode and an anode support part, and a cathode chamber, one side of which contacts a surface of the ion exchange membrane opposite the anode chamber, wherein the cathode chamber further comprises a cathode, wherein the ladder-shaped anode is formed on a first surface of the anode support part, openings are formed in the anode support part which conform to the shape of the ladder-shaped anode, and a second surface of the anode support part which opposes the first surface of the anode contacts and supports the ion exchange membrane.

    摘要翻译： 微流体装置包括： 离子交换膜，阳极室，其一面接触离子交换膜的表面，其中阳极室还包括梯形阳极和阳极支撑部分，阴极室的一侧接触表面 所述阴极室还包括阴极，其中所述梯形阳极形成在所述阳极支撑部分的第一表面上，所述阳极支撑部分形成符合所述形状的阳极支撑部分的形状， 并且与阳极的第一表面相对的阳极支撑部分的第二表面接触并支撑离子交换膜。

    MICROFLUIDIC CHIP AND METHOD OF FABRICATING THE SAME
    6.
    发明申请
    MICROFLUIDIC CHIP AND METHOD OF FABRICATING THE SAME 有权
    微流控芯片及其制造方法

    公开(公告)号：US20110081280A1

    公开(公告)日：2011-04-07

    申请号：US12949981

    申请日：2010-11-19

    申请人： Kyu-youn HWANG ,  Joon-ho KIM ,  Chin-sung PARK ,  Sung-young JEONG

    发明人： Kyu-youn HWANG ,  Joon-ho KIM ,  Chin-sung PARK ,  Sung-young JEONG

    IPC分类号： B01L3/00

    CPC分类号： B01L3/502707 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/16 ,  Y10T428/14

    摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

    摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。