公开(公告)号：US08697441B2

公开(公告)日：2014-04-15

申请号：US13510528

申请日：2010-10-08

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

IPC分类号： C12N5/071

CPC分类号： C12N5/0667 ,  C12N2500/36 ,  C12N2502/1305

摘要： The present invention relates to a method of inducing high activity of human adipose stem cells, highly active stem cells induced by the method, cell therapeutic agents including the highly active stem cells, and a medium for inducing high activity of human adipose stem cells. The method of the present invention enables a long-term culture of human adipose stem cells while maintaining high activity, production yield and differentiation potency of the stem cells through in vitro culture, even in case culture conditions are not appropriate for mature human adipocytes, security of adipocytes is not guaranteed, or adipocytes are diseased.

摘要翻译： 本发明涉及一种诱导人类脂肪干细胞高活性的方法，由该方法诱导的高活性干细胞，包括高活性干细胞的细胞治疗剂和诱导人类脂肪干细胞高活性的培养基。 本发明的方法能够通过体外培养保持人体脂肪干细胞的长期培养，同时通过体外培养保持干细胞的高活性，产量和分化效能，即使培养条件不适合成熟的人类脂肪细胞，安全性 的脂肪细胞不能保证，或脂肪细胞患病。

公开(公告)号：US20110142809A1

公开(公告)日：2011-06-16

申请号：US13056279

申请日：2009-07-17

申请人： Hyo Soo Kim ,  Eun-Ju Lee ,  Hyun-Jae Kang

发明人： Hyo Soo Kim ,  Eun-Ju Lee ,  Hyun-Jae Kang

IPC分类号： A61K35/12 ,  C12N5/071 ,  A61P43/00

CPC分类号： C12N5/0665 ,  C12N5/0662 ,  C12N2500/90

摘要： The present invention relates to a method for separating highly active stem cells from human stem cells, the highly active stem cells separated by the method, a cell therapeutic agent containing the stem cells, and a medium for separating the highly active stem cells from stem cells containing a specific cytokine. According to the present invention, the method is useful for separating the highly efficient stem cells from mesenchymal stem cells of various origins. Further, the method is very useful in developing a cell therapeutic agent of high efficiency because the method can be applicable to stem cells of various origins which are cultured under different conditions. Senescent stem cells increased by several passage times in vitro can be effectively sorted out, so the method can be used for reactivating the stem cells.

摘要翻译： 本发明涉及从人类干细胞分离高活性干细胞的方法，通过该方法分离的高活性干细胞，含有干细胞的细胞治疗剂和用于从干细胞分离高活性干细胞的培养基 含有特异性细胞因子。 根据本发明，该方法可用于从各种来源的间充质干细胞分离高效的干细胞。 此外，该方法对于开发高效率的细胞治疗剂非常有用，因为该方法可适用于在不同条件下培养的各种来源的干细胞。 衰老干细胞在体外增加数次传代次数可以有效地分选出来，因此该方法可用于再生干细胞。

公开(公告)号：US09388384B2

公开(公告)日：2016-07-12

申请号：US13504881

申请日：2009-10-28

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

IPC分类号： C12N5/00 ,  C12N5/0775 ,  A61K35/48

CPC分类号： C12N5/0662 ,  A61K35/48 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/39 ,  C12N2506/02

摘要： Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.

摘要翻译： 提供一种从人多能干细胞产生间充质干细胞的方法，所述方法包括：a）从人多能干细胞形成胚体; b）将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 和c）对间充质干细胞进行连续增殖培养，同时仍保持间充质干细胞的特性。 此外，提供了诱导间充质干细胞分化的标准化方法，其可以广泛地应用于所有人多能干细胞，而与其遗传背景的差异无关。 最终，本发明可以通过使用人多能干细胞连续批量生产再生医学和细胞治疗所需的间充质干细胞，从而实现细胞治疗产品的实际应用，并且进一步预期本发明对 不治之症，如心血管疾病和神经系统疾病。

公开(公告)号：US20130023048A1

公开(公告)日：2013-01-24

申请号：US13510528

申请日：2010-10-08

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

IPC分类号： C12N5/0775

CPC分类号： C12N5/0667 ,  C12N2500/36 ,  C12N2502/1305

摘要： The present invention relates to a method of inducing high activity of human adipose stem cells, highly active stem cells induced by the method, cell therapeutic agents including the highly active stem cells, and a medium for inducing high activity of human adipose stem cells.The method of the present invention enables a long-term culture of human adipose stem cells while maintaining high activity, production yield and differentiation potency of the stem cells through in vitro culture, even in case culture conditions are not appropriate for mature human adipocytes, security of adipocytes is not guaranteed, or adipocytes are diseased.

摘要翻译： 本发明涉及一种诱导人类脂肪干细胞高活性的方法，由该方法诱导的高活性干细胞，包括高活性干细胞的细胞治疗剂和诱导人类脂肪干细胞高活性的培养基。 本发明的方法能够通过体外培养保持人体脂肪干细胞的长期培养，同时通过体外培养保持干细胞的高活性，产量和分化效能，即使培养条件不适合成熟的人类脂肪细胞，安全性 的脂肪细胞不能保证，或脂肪细胞患病。

公开(公告)号：US20120276625A1

公开(公告)日：2012-11-01

申请号：US13504881

申请日：2009-10-28

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

IPC分类号： C12N5/0775

CPC分类号： C12N5/0662 ,  A61K35/48 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/39 ,  C12N2506/02

摘要： Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.

摘要翻译： 提供一种从人多能干细胞产生间充质干细胞的方法，所述方法包括：a）从人多能干细胞形成胚体; b）将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 和c）对间充质干细胞进行连续增殖培养，同时仍保持间充质干细胞的特性。 此外，提供了诱导间充质干细胞分化的标准化方法，其可以广泛地应用于所有人多能干细胞，而与其遗传背景的差异无关。 最终，本发明可以通过使用人多能干细胞连续批量生产再生医学和细胞治疗所需的间充质干细胞，从而实现细胞治疗产品的实际应用，并且进一步预期本发明对 不治之症，如心血管疾病和神经系统疾病。

公开(公告)号：US09139811B2

公开(公告)日：2015-09-22

申请号：US13981508

申请日：2011-12-27

申请人： Eun Ju Lee ,  Hyo Soo Kim

发明人： Eun Ju Lee ,  Hyo Soo Kim

IPC分类号： C12N5/071 ,  C12N5/00 ,  C12N5/0775 ,  A61K31/4409

CPC分类号： C12N5/0018 ,  A61K31/4409 ,  C12N5/0663 ,  C12N2501/727

摘要： The present invention relates to a composition for suppressing the death of stem cells during suspension culturing, a composition for mass culturing of stem cells, and a method for suppressing cell death of stem cells during suspension culturing. The composition of the present invention can be usefully used for effective single suspension culturing of stem cells, particularly, mesenchymal stem cells, or used for mass suspension culturing under the circumstances in which cell aggregation does not occur.

摘要翻译： 本发明涉及一种用于抑制悬浮培养期间干细胞死亡的组合物，用于干细胞的大规模培养的组合物，以及用于在悬浮培养期间抑制干细胞的细胞死亡的方法。 本发明的组合物可有效地用于干细胞，特别是间充质干细胞的有效单悬液培养，或用于在不发生细胞聚集的情况下进行大规模悬浮培养。

公开(公告)号：US20140011275A1

公开(公告)日：2014-01-09

申请号：US13981508

申请日：2011-12-27

申请人： Eun Ju Lee ,  Hyo Soo Kim

发明人： Eun Ju Lee ,  Hyo Soo Kim

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  A61K31/4409 ,  C12N5/0663 ,  C12N2501/727

摘要： The present invention relates to a composition for suppressing the death of stem cells during suspension culturing, a composition for mass culturing of stem cells, and a method for suppressing cell death of stem cells during suspension culturing. The composition of the present invention can be usefully used for effective single suspension culturing of stem cells, particularly, mesenchymal stem cells, or used for mass suspension culturing under the circumstances in which cell aggregation does not occur.

摘要翻译： 本发明涉及一种用于抑制悬浮培养期间干细胞死亡的组合物，用于干细胞的大规模培养的组合物，以及用于在悬浮培养期间抑制干细胞的细胞死亡的方法。 本发明的组合物可有效地用于干细胞，特别是间充质干细胞的有效单悬液培养，或用于在不发生细胞聚集的情况下进行大规模悬浮培养。

公开(公告)号：US09933424B2

公开(公告)日：2018-04-03

申请号：US14237518

申请日：2012-08-07

申请人： Young Bae Park ,  Hyo Soo Kim ,  Yoo Wook Kwon ,  Sahmin Lee ,  Hyun Chae Lee ,  Young Jin Cho ,  Sang Eun Lee ,  Jun Ho Chung

发明人： Young Bae Park ,  Hyo Soo Kim ,  Yoo Wook Kwon ,  Sahmin Lee ,  Hyun Chae Lee ,  Young Jin Cho ,  Sang Eun Lee ,  Jun Ho Chung

IPC分类号： G01N33/53 ,  G01N33/564 ,  G01N33/68

CPC分类号： G01N33/564 ,  G01N33/6863 ,  G01N2333/715

摘要： The present invention concerns a human resistin receptor. More particularly, the present invention provides a method for screening a receptor of human resistin protein, a method for preventing or treating an inflammatory disease and arteriosclerosis using an expression- or activity-regulator for a human resistin receptor, and a pharmaceutical composition including an expression- or activity-regulator for the human resistin receptor. The method for screening a human resistin protein receptor according to the present invention enables separation of a receptor which directly binds to resistin from human monocyte, reveals a mechanism of signal transduction of the resistin receptor, and therefore, is expected to contribute to regulation of an inflammatory effect of monocyte, molecular detection of causes for vascular inflammation and arteriosclerosis, and developments of prevention and a treating agent for an inflammatory disease and arteriosclerosis.

公开(公告)号：US20120214804A1

公开(公告)日：2012-08-23

申请号：US13503857

申请日：2010-10-25

申请人： Soon Ha Kim ,  Hyoung Jin Kim ,  Heui Sul Park ,  Seon Yeong Gu ,  Hyo Shin Kwak ,  Du Hee Park ,  Hyo Soo Kim ,  Hyun Jai Cho ,  Ji Hyun Kim ,  Ju Young Kim ,  Kwang Min Park

发明人： Soon Ha Kim ,  Hyoung Jin Kim ,  Heui Sul Park ,  Seon Yeong Gu ,  Hyo Shin Kwak ,  Du Hee Park ,  Hyo Soo Kim ,  Hyun Jai Cho ,  Ji Hyun Kim ,  Ju Young Kim ,  Kwang Min Park

IPC分类号： A61K31/4184 ,  A61P29/00 ,  A61P31/00 ,  A61P19/02 ,  A61P1/16 ,  A61P7/04 ,  A61P31/12 ,  A61P31/04 ,  A61P9/00 ,  A61P25/28 ,  A61P9/06 ,  A61P25/14 ,  A61P25/16 ,  A61P31/16 ,  A61P31/20 ,  A61P31/18 ,  A61K8/49 ,  C07D235/18 ,  C07D405/10 ,  A61K31/427 ,  C07D417/04 ,  A61K31/422 ,  C07D413/04 ,  A61K31/496 ,  C07D417/14 ,  A61K31/4985 ,  C07D487/04 ,  A61K31/541 ,  A61P9/10

CPC分类号： A61K31/541 ,  A61K8/492 ,  A61K31/404 ,  A61K31/4184 ,  A61K31/427 ,  A61K31/496 ,  A61K31/497 ,  A61K2800/522 ,  A61Q19/00 ,  C07D413/04 ,  C07D417/04 ,  C07D417/14 ,  C07D471/04 ,  C07D487/04

摘要： The present invention relates to a pharmaceutical composition comprising a compound of formula (1), (2) or (3) as defined in the specification for the prevention or treatment of diseases associated with oxidative stress, mitochondria dysfunction, hypoxic injury, necrosis and/or ischemic reperfusion injury, and a cosmetic composition comprising an indole compound having an antioxidant effect.

摘要翻译： 本发明涉及药物组合物，其包含如说明书中定义的式（1），（2）或（3）化合物，其用于预防或治疗与氧化应激，线粒体功能障碍，缺氧损伤，坏死和/ 或缺血再灌注损伤，以及包含具有抗氧化作用的吲哚化合物的化妆品组合物。

公开(公告)号：US20140171489A1

公开(公告)日：2014-06-19

申请号：US14237518

申请日：2012-08-07

申请人： Young Bae Park ,  Hyo Soo Kim ,  Yoo Wook Kwon ,  Sahmin Lee ,  Hyun Chae Lee ,  Young Jin Cho ,  Sang Eun Lee ,  Jun Ho Chung

发明人： Young Bae Park ,  Hyo Soo Kim ,  Yoo Wook Kwon ,  Sahmin Lee ,  Hyun Chae Lee ,  Young Jin Cho ,  Sang Eun Lee ,  Jun Ho Chung

IPC分类号： G01N33/569

CPC分类号： G01N33/564 ,  G01N33/6863 ,  G01N2333/715

摘要： The present invention concerns a human resistin receptor. More particularly, the present invention provides a method for screening a receptor of human resistin protein, a method for preventing or treating an inflammatory disease and arteriosclerosis using an expression- or activity-regulator for a human resistin receptor, and a pharmaceutical composition including an expression- or activity-regulator for the human resistin receptor. The method for screening a human resistin protein receptor according to the present invention enables separation of a receptor which directly binds to resistin from human monocyte, reveals a mechanism of signal transduction of the resistin receptor, and therefore, is expected to contribute to regulation of an inflammatory effect of monocyte, molecular detection of causes for vascular inflammation and arteriosclerosis, and developments of prevention and a treating agent for an inflammatory disease and arteriosclerosis.

摘要翻译： 本发明涉及人抵抗素受体。 更具体地，本发明提供了筛选人抵抗素蛋白的受体的方法，使用人抵抗素受体的表达或活性调节剂预防或治疗炎症性疾病和动脉硬化的方法，以及包含表达 - 或人抵抗素受体的活性调节剂。 根据本发明的用于筛选人抵抗素蛋白受体的方法使得能够从人单核细胞中分离直接结合抵抗素的受体，揭示了抵抗素受体信号转导的机制，因此预期有助于调节 单核细胞的炎症作用，血管炎症和动脉硬化原因的分子检测，以及预防和炎性疾病和动脉硬化治疗剂的发展。