公开(公告)号：US20250059521A1

公开(公告)日：2025-02-20

申请号：US18824208

申请日：2024-09-04

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US12104182B2

公开(公告)日：2024-10-01

申请号：US18120639

申请日：2023-03-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20230357731A1

公开(公告)日：2023-11-09

申请号：US18120639

申请日：2023-03-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Kay Klausing ,  Hamed Tabatabaei Ghomi ,  Misha Golynskiy ,  Saurabh Nirantar ,  Seth McDonald ,  Sergio Peisajovich

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US11560552B2

公开(公告)日：2023-01-24

申请号：US17397003

申请日：2021-08-09

Applicant: Illumina, Inc. ,  Illumina Singapore Pte. Ltd.

Inventor： Misha Golynskiy ,  Seth McDonald ,  Saurabh Nirantar ,  Matthew Kellinger ,  Michael Previte ,  Sergio Peisajovich ,  Molly He

IPC: C12N9/12 ,  C12N15/52 ,  C12N1/20 ,  C12Q1/68

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US20220056424A1

公开(公告)日：2022-02-24

申请号：US17397003

申请日：2021-08-09

Applicant: Illumina, Inc. ,  Illumina Singapore Pte. Ltd.

Inventor： Misha Golynskiy ,  Seth McDonald ,  Saurabh Nirantar ,  Matthew Kellinger ,  Michael Previte ,  Sergio Peisajovich ,  Molly He

IPC: C12N9/12

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US10745751B2

公开(公告)日：2020-08-18

申请号：US16541942

申请日：2019-08-15

Applicant: ILLUMINA, INC.

Inventor： Erin Bomati ,  Molly He ,  Cheng-Yao Chen ,  Sergio Peisajovich

IPC: C12Q1/6869 ,  C12N9/12 ,  C12Q1/6844

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：US12110516B2

公开(公告)日：2024-10-08

申请号：US17317721

申请日：2021-05-11

Applicant: Illumina, Inc. ,  Illumina Singapore Pte. Ltd. ,  National University of Singapore

Inventor： Saurabh Rajendra Nirantar ,  Jasmine Puay Suan Chua ,  Sergio Peisajovich ,  Wen Shan Yew ,  Maybelle Darlene Kho Go

IPC: C12N9/12

CPC classification number: C12N9/1264 ,  C12Y207/07031

Abstract: Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His421 in the bovine TdT.

公开(公告)号：US20210147927A1

公开(公告)日：2021-05-20

申请号：US16929632

申请日：2020-07-15

Applicant: ILLUMINA, INC.

Inventor： Erin Bomati ,  Molly He ,  Cheng-Yao Chen ,  Sergio Peisajovich

IPC: C12Q1/6869 ,  C12N9/12 ,  C12Q1/6844

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：US10995324B2

公开(公告)日：2021-05-04

申请号：US16403976

申请日：2019-05-06

Applicant: Illumina, Inc.

Inventor： Misha Golynskiy ,  Molly He ,  Michael Previte ,  BeiBei Wang ,  Sergio Peisajovich

IPC: C12N9/12 ,  C12P19/34

Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.

公开(公告)号：US10851131B2

公开(公告)日：2020-12-01

申请号：US16627035

申请日：2019-02-19

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Jeffrey Mandell ,  Silvia Gravina ,  Sergio Peisajovich ,  Kaitlin Pugliese ,  Yin Nah Teo ,  Xiangyuan Yang ,  Maria Candelaria Rogert Bacigalupo

IPC: C07H21/00 ,  C12Q1/6869 ,  C40B70/00

Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I: