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    Dynamic autofocus method and system for assay imager
    1.
    发明授权
    Dynamic autofocus method and system for assay imager 有权

    公开(公告)号：US12153210B2

    公开(公告)日：2024-11-26

    申请号：US17302418

    申请日：2021-05-03

    Applicant: Illumina, Inc.

    Inventor： Darren Robert Segale ,  John Moon ,  Hongji Ren

    IPC: G02B26/12 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01B9/02055 ,  G01B11/24 ,  G01N15/1434 ,  G01N21/64 ,  G02B7/38 ,  G02B21/24 ,  G02B26/10 ,  H04N23/67

    Abstract: A method for scanning a microarray, including (a) providing a microarray to an optical scanner, wherein the microarray includes a surface having features having different target molecules; (b) scanning the surface in the x or y direction to acquire optical signals from the features at sequential regions of the surface, wherein the focus setting between the optical scanner and the surface is dynamically controlled in real time by repeatedly (i) adding a predetermined focal offset, thereby introducing an error in the focus setting, and (ii) adjusting the focus setting to reduce the error in the focus setting, thereby acquiring the optical signals from different regions at different degrees of focus such that a subset of the regions that are scanned have an introduced focus error; and (c) analyzing the optical signals that are obtained at the different degrees of focus to identify the different target molecules.

    POLYMER COATINGS
    3.
    发明申请
    POLYMER COATINGS 有权

    公开(公告)号：US20210254151A1

    公开(公告)日：2021-08-19

    申请号：US17188109

    申请日：2021-03-01

    Applicant: Illumina, Inc.

    Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan Christopher Smith

    IPC: C12Q1/6874 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806

    Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

    Polymer coatings
    4.
    发明授权
    Polymer coatings 有权

    公开(公告)号：US10954561B2

    公开(公告)日：2021-03-23

    申请号：US16380832

    申请日：2019-04-10

    Applicant: Illumina, Inc.

    Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan Christopher Smith

    IPC: C12Q1/68 ,  C12Q1/6874 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806

    Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

    POLYMER COATINGS
    6.
    发明申请
    POLYMER COATINGS 有权
    聚合物涂料

    公开(公告)号：US20150259735A1

    公开(公告)日：2015-09-17

    申请号：US14679760

    申请日：2015-04-06

    Applicant: Illumina, Inc.

    Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan C. Smith

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  B05D3/06 ,  B05D3/065 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806 ,  Y10T428/24802 ,  Y10T428/24917 ,  Y10T428/24926 ,  Y10T428/2998 ,  Y10T428/31536 ,  Y10T428/31935

    Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

    Abstract translation: 本公开内容涉及共价连接到基材表面上的聚合物涂层以及聚（N-（5-叠氮基乙酰胺基戊基）丙烯酰胺 - 共 - 丙烯酰胺）聚合物涂层的制备（PAZAM），其形成和操作 底物，如分子阵列和流动池。 本公开还涉及通过使用涂覆有共价连接的聚合物（例如PAZAM）的珠来制备底物表面的方法，以及测定与本文所述的底物表面连接的多核苷酸的核苷酸序列的方法。

    Apparatus and method of estimating values from images
    7.
    发明授权
    Apparatus and method of estimating values from images 有权

    公开(公告)号：US12204086B2

    公开(公告)日：2025-01-21

    申请号：US18236088

    申请日：2023-08-21

    Applicant: ILLUMINA, INC.

    Inventor： Robert Langlois ,  Bo Lu ,  Hongji Ren ,  Joseph Pinto ,  Simon Prince ,  Austin Corbett

    IPC: G02B21/36 ,  G02B21/14 ,  G06T7/00

    Abstract: A method is used to generate a distortion model for a structured illumination microscopy (SIM) optical system. A sliding window is moved in relation to a plurality of images to define a plurality of sub-tiles. Each sub-tile represents a portion of the corresponding image. Parameters are estimated for each sub-tiles. The parameters include two or more parameters selected from the group consisting of modulation, angle, spacing, phase offset, and phase deviation. A full width at half maximum (FWHM) value associated with each sub-tile is estimated. A distortion model is estimated, based at least in part on a combination of the estimated parameters and FWHM values stored in the predetermined format and an estimated center window parameter. A two-dimensional image may be generated, based at least in part on the estimated distortion model. The two-dimensional image may include representations indicating where distortions occur in the optical system.

    PERFORMING STRUCTURED ILLUMINATION MICROSCOPY ON A PATTERNED SUBSTRATE
    9.
    发明申请
    PERFORMING STRUCTURED ILLUMINATION MICROSCOPY ON A PATTERNED SUBSTRATE 有权

    公开(公告)号：US20220299744A1

    公开(公告)日：2022-09-22

    申请号：US17654941

    申请日：2022-03-15

    Applicant: Illumina, Inc.

    Inventor： Hongji Ren ,  John Moon

    IPC: G02B21/00 ,  G02B21/06

    Abstract: A structured illumination microscopy (SIM) system comprises: a light source; a light-structuring component to provide light from the light source with a SIM pattern for performing illuminations of a sample at a substrate having a substrate pattern, wherein a pitch of the SIM pattern is based on a characteristic of the substrate pattern; and an image sensor to detect emissions that the sample generates in response to the illuminations.

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