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    Cryoprotectants for freeze drying of lactic acid bacteria
    1.
    发明授权
    Cryoprotectants for freeze drying of lactic acid bacteria 有权

    公开(公告)号：US10596207B2

    公开(公告)日：2020-03-24

    申请号：US15398945

    申请日：2017-01-05

    申请人： INTREXON ACTOBIOTICS NV

    发明人： Sam Corveleyn ,  Patrick Dhaese ,  Sabine Neirynck ,  Lothar Steidler

    IPC分类号： A61K35/744 ,  C12N1/04 ,  A23K20/10 ,  A23L33/10 ,  A23L33/135 ,  A61K47/10 ,  A61K47/12 ,  A61K47/36 ,  C12N1/20

    摘要： The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications. More in particular, the invention relates to the increased viability and long-term storage of recombinant bacteria capable of expressing heterologous proteins or peptides and administered to humans or animals for therapeutic or vaccination purposes.

    MODIFIED GRAM POSITIVE BACTERIA AND USES THEREOF
    5.
    发明申请
    MODIFIED GRAM POSITIVE BACTERIA AND USES THEREOF 审中-公开
    改良GRAM阳性细菌及其用途

    公开(公告)号：US20160376574A1

    公开(公告)日：2016-12-29

    申请号：US15258433

    申请日：2016-09-07

    申请人： INTREXON ACTOBIOTICS NV

    发明人： Lothar Steidler ,  Karolien Van Huynegem ,  Klaas Vandenbroucke

    IPC分类号： C12N9/10 ,  A23L33/135 ,  C12P19/12 ,  A61K35/744 ,  C12N1/20 ,  C12N15/74

    CPC分类号： C12N9/1051 ,  A23L33/135 ,  A23V2002/00 ,  A61K35/744 ,  A61K38/00 ,  A61K2039/522 ,  C07K14/325 ,  C12N1/04 ,  C12N1/20 ,  C12N15/746 ,  C12N15/86 ,  C12P19/12 ,  C12Y204/01216

    摘要： The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.

    摘要翻译： 本发明涉及革兰氏阳性菌，优选乳酸菌（LAB）或双歧杆菌，具有增加的抗逆性和/或改进的制造，加工和/或储存特性。 特别地，本发明涉及一种积累细胞内海藻糖的革兰氏阳性细菌。 根据本发明的革兰氏阳性细菌缺乏海藻糖-6-磷酸磷酸化酶（TrePP）活性。 革兰氏阳性细菌可能进一步缺乏纤维二糖特异性PTS系统IIC成分（ptcC）活性。 革兰氏阳性菌可能进一步过表达海藻糖转运蛋白。 本发明还涉及包含这种革兰氏阳性细菌的组合物及其方法和用途。

    Genetically Modified Bacteria Stably Expressing IL-10 and Insulin
    8.
    发明公开
    Genetically Modified Bacteria Stably Expressing IL-10 and Insulin 审中-公开

    公开(公告)号：US20230348918A1

    公开(公告)日：2023-11-02

    申请号：US18059689

    申请日：2022-11-29

    申请人： Intrexon Actobiotics NV

    发明人： Pieter Rottiers ,  Lothar Steidler

    IPC分类号： C12N15/74 ,  A61K35/744 ,  A61K39/395 ,  C07K16/28 ,  C07K14/54 ,  C07K14/62 ,  A61P3/10

    CPC分类号： C12N15/746 ,  A61K35/744 ,  A61K39/3955 ,  A61P3/10 ,  C07K14/5428 ,  C07K14/62 ,  C07K16/2809 ,  C12N15/74

    摘要： The current disclosure provides microorganisms, such as lactic acid bacteria (e.g., Lactococcus lactis) containing an exogenous nucleic acid encoding an IL-10 polypeptide and an exogenous nucleic acid encoding a T1D-specific antigen (e.g., a proinsulin) polypeptide, wherein both exogenous nucleic acids are integrated into the bacterial chromosome. Such microbial strains are suitable for human therapy. The disclosure further provides compositions (e.g., pharmaceutical compositions) methods of using the microorganisms and compositions, e.g., for the treatment of type 1 diabetes (T1D), including those with residual beta-cell function, e.g., recent-onset T1D. The microorganism may be administered orally, delivers the microorganism into the gastrointestinal tract, where it is released and expresses the bioactive polypeptides. The methods of the present disclosure are particularly well suited for subjects possessing residual beta-cell function, e.g., for subjects with recent-onset T1D.

    Cryoprotectants for freeze drying of lactic acid bacteria
    9.
    发明授权
    Cryoprotectants for freeze drying of lactic acid bacteria 有权

    公开(公告)号：US11633438B2

    公开(公告)日：2023-04-25

    申请号：US16782372

    申请日：2020-02-05

    申请人： INTREXON ACTOBIOTICS NV

    发明人： Sam Corveleyn ,  Patrick Dhaese ,  Sabine Neirynck ,  Lothar Steidler

    IPC分类号： A61K35/744 ,  C12N1/04 ,  A23K20/10 ,  A23L33/10 ,  A23L33/135 ,  A61K47/10 ,  A61K47/12 ,  A61K47/36 ,  C12N1/20

    摘要： The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications. More in particular, the invention relates to the increased viability and long-term storage of recombinant bacteria capable of expressing heterologous proteins or peptides and administered to humans or animals for therapeutic or vaccination purposes.