公开(公告)号：US20030228700A1

公开(公告)日：2003-12-11

申请号：US10289009

申请日：2002-11-05

Applicant: IRM LLC

Inventor： Eric C. Peters ,  Ansgar Brock ,  Christer Ericson

IPC: G01N033/00

CPC classification number: C07K1/13 ,  C07K14/805 ,  G01N33/6848 ,  G01N2035/00158 ,  G01N2458/15 ,  G16B30/00 ,  G16B50/00 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/38 ,  Y10T436/24

Abstract: The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

Abstract translation: 本发明提供在蛋白质组学研究中可用作多功能标记的化合物。 本发明的标记物都是赖氨酸特异性的，并且通过例如提高赖氨酸终止的胰蛋白酶片段的离子化效率来增加多肽测绘实验中获得的整体序列覆盖率。 在某些方面，本发明的标记可以用于测量差异定量，例如，在合成过程中可以容易地引入氘。 在一个方面，C末端衍生的赖氨酸强烈地将片段离子强度偏向C末端片段离子，导致高度简化的串联质谱。 在另外的方面，可以在多肽中确定赖氨酸残基的数目。

公开(公告)号：US20030139885A1

公开(公告)日：2003-07-24

申请号：US10289462

申请日：2002-11-05

Applicant: IRM, LLC

Inventor： Ansgar Brock ,  David M. Horn ,  Eric C. Peters

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N33/6848 ,  B82Y30/00 ,  G01N33/6818 ,  G01N33/6842 ,  G01N33/6851 ,  G01N2035/00158 ,  G01N2458/15 ,  G16B30/00 ,  G16B50/00 ,  H01J49/0036

Abstract: Provided are methods and systems for identification of proteins using high mass accuracy mass spectrometry. Not only do high mass accuracy measurements provide greater confidence in protein identification assignments, but they also enable proteins to be identified with either less sequence coverage or fewer additional tandem MS experiments. In addition, high mass measurement accuracy optionally allows protein identifications to be made on the basis of the mass of a single peptide, providing higher-throughputs in the analysis of mixtures due to the significant decrease in time spent on additional tandem MS experiments. In addition, a concomitant time saving in the cross correlation process of mass spectral data with in silico digested databases would also be achieved.

Abstract translation: 提供了使用高质量精度质谱法鉴定蛋白质的方法和系统。 不仅高质量精度测量对蛋白质鉴定分配提供了更大的信心，而且还使蛋白质能够以更少的序列覆盖率或更少的附加串联MS实验来鉴定。 此外，高质量测量精度可选地允许基于单个肽的质量进行蛋白质鉴定，由于在额外的串联MS实验中花费的时间的显着减少，在混合物的分析中提供更高的通量。 此外，也可以实现在计算机数据库中同时节省质谱数据的互相关过程。

公开(公告)号：US20030138823A1

公开(公告)日：2003-07-24

申请号：US10289160

申请日：2002-11-05

Applicant: IRM, LLC

Inventor： Ansgar Brock ,  Qui Phung ,  Eric C. Peters

IPC: C12Q001/68

CPC classification number: G01N33/6848 ,  B82Y30/00 ,  G01N33/6842 ,  G01N33/6851 ,  G01N2035/00158 ,  G01N2458/15 ,  G16B30/00 ,  G16B50/00 ,  H01J49/0009 ,  H01J49/0418

Abstract: This invention provides methods for preparing samples for MALDI mass spectrometry. The methods make possible the use of reagents that are normally considered unsuitable for MALDI in the preparation of a sample. Also provided are methods for internal calibration of a mass spectrometer, and methods for preparing sample supports.

Abstract translation: 本发明提供用于制备用于MALDI质谱的样品的方法。 这些方法使得可以使用通常认为不适合于MALDI制备样品的试剂。 还提供了用于质谱仪的内部校准的方法以及用于制备样品载体的方法。