摘要:
The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor operates without additional development reagents, requiring only the presence of analyte or target for function.
摘要:
A method of controllably changing an intrinsic property of a quantum dot by using a biological entity, either attached or in close proximity to the quantum dot, and changing the state of biological entity with a controllable mechanism. The change in state of the biological entity controllably changes the intrinsic property of the quantum dot. The photoluminescence emission of quantum dots can be controlled by the present method. The methods disclosed include controlling the magnitude of QD photoluminescence as well as turning the photoluminescence on/off. The methods disclosed include using the same biological control architecture to control other intrinsic QD properties such as charge state, magnetic or other property.
摘要:
A nanoparticle having a surfactant shell with a hydrophilic outer surface and a hydrophobic inner surface and an organic chromophore and a polymer having aromatic groups within the surfactant shell. A method of making nanoparticles by: emulsifying an aqueous composition of a surfactant and an organic solution of a monomer and an organic chromophore to form micelles of the monomer and the chromophore inside a surfactant shell; and polymerizing the monomer. A method of: reacting a ω-bromoalkyl acid with acryloyl acid lithium salt, and reacting the product with sodium hydride to produce an acryloyloxyalkyl carboxylic acid sodium salt. The compound shown below.
摘要:
A method of biochemical identification by: providing a plurality of capture species bound to one or more substrates and suspected of having one or more biological targets affinity bound to at least one capture species; detecting which capture species contain bound biological targets to generate a binding pattern; and identifying the biological target based on the binding pattern. The capture species are independently selected from the group consisting of antimicrobial peptides, cytotoxic peptides, antibiotics, and combinations thereof. A device having the capture species bound to the substrates. At least two of the capture species are capable of multi-specific binding to one or more biological targets and may have overlapping but not identical affinity properties.