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    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making
    2.
    发明授权
    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making 有权
    具有可调节差异结合亲和力和制备方法的无菌和可重复使用的生物传感器

    公开(公告)号：US07927547B2

    公开(公告)日：2011-04-19

    申请号：US12103778

    申请日：2008-04-16

    申请人： Igor L Medintz ,  J Matthew Mauro ,  Ellen R Goldman ,  George P Anderson

    发明人： Igor L Medintz ,  J Matthew Mauro ,  Ellen R Goldman ,  George P Anderson

    IPC分类号： G01N21/00 ,  G01N21/01 ,  G01N21/64 ,  G01N33/53 ,  G01N33/553

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可运行，仅需要分析物或靶标的存在才能发挥作用。

    Affinity-based detection of biological targets
    3.
    发明授权
    Affinity-based detection of biological targets 有权
    基于亲和力的生物靶标检测

    公开(公告)号：US08658372B2

    公开(公告)日：2014-02-25

    申请号：US11307399

    申请日：2006-02-06

    申请人： Nadezhda V Kulagina ,  Chris Rowe Taitt ,  George P Anderson ,  Frances S Ligler

    发明人： Nadezhda V Kulagina ,  Chris Rowe Taitt ,  George P Anderson ,  Frances S Ligler

    IPC分类号： G01N33/53

    CPC分类号： G01N33/56916 ,  G01N33/5308 ,  G01N33/54366 ,  G01N33/554 ,  G01N33/56911 ,  G01N33/56983

    摘要： A method of biochemical identification by: providing a plurality of capture species bound to one or more substrates and suspected of having one or more biological targets affinity bound to at least one capture species; detecting which capture species contain bound biological targets to generate a binding pattern; and identifying the biological target based on the binding pattern. The capture species are independently selected from the group consisting of antimicrobial peptides, cytotoxic peptides, antibiotics, and combinations thereof. A device having the capture species bound to the substrates. At least two of the capture species are capable of multi-specific binding to one or more biological targets and may have overlapping but not identical affinity properties.

    摘要翻译： 生物化学鉴定的方法是：提供与一种或多种底物结合的多种捕获物质，并怀疑具有与至少一种捕获物质结合的一种或多种生物靶标; 检测哪些捕获物种含有结合的生物靶标以产生结合模式; 以及基于所述结合模式识别所述生物学目标。 捕获物种独立地选自抗微生物肽，细胞毒性肽，抗生素及其组合。 具有捕获物质与基底结合的装置。 至少两种捕获物质能够与一种或多种生物靶标进行多特异性结合，并且可以具有重叠但不相同的亲和性质。