公开(公告)号：US20050084909A1

公开(公告)日：2005-04-21

申请号：US10928319

申请日：2004-08-30

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu

IPC分类号： C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/581 ,  G01N33/54306

摘要： An antigen measuring device has an antibody chip. The antibody chip has a substrate. A pair of gratings are formed on the substrate. An antibody-fixed layer surrounded by a wall is formed between the gratings. A light emitting element emits a light beam toward the first grating. A light receiving element receives the light beam which propagates through the substrate under the antibody-fixed layer and is outputfrom the second grating.

摘要翻译： 抗原测定装置具有抗体芯片。 抗体芯片具有底物。 在基板上形成一对光栅。 在光栅之间形成由壁包围的抗体固定层。 发光元件朝向第一光栅发射光束。 光接收元件接收在抗体固定层下传播通过衬底并从第二光栅输出的光束。

公开(公告)号：US07498145B2

公开(公告)日：2009-03-03

申请号：US11363094

申请日：2006-02-28

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

IPC分类号： G01N33/53 ,  G01N33/551 ,  G01N33/552 ,  G01N33/58

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： The method for measuring the concentration of the measuring object uses a sensor chip comprising an optical waveguide layer and an antibody immobilized layer formed on the surface of the optical waveguide layer, which comprises immobilizing the measuring object and an enzyme-labeled antibody labeled with a labeling enzyme on the antibody immobilized layer of the sensor chip having an immobilized antibody, producing a color-developing and precipitating enzyme reaction product by allowing to react a coloring reagent with the labeling enzyme on the antibody immobilized layer to precipitate the enzyme reaction product on the antibody immobilized layer, allowing to totally reflect a light impinged on the sensor chip from the outside at an interface between the optical waveguide layer and the antibody immobilized layer, and observing to a physical value of the totally reflected light.

摘要翻译： 用于测量对象浓度的方法使用包括形成在光波导层表面上的光波导层和抗体固定层的传感器芯片，其包括固定测量对象和用​​标记的酶标记的抗体 酶在具有固定化抗体的传感器芯片的抗体固定化层上，通过使着色试剂与抗体固定化层上的标记酶反应，使酶反应产物沉淀在抗体上，产生显色和沉淀酶反应产物 使得能够在光波导层和抗体固定化层之间的界面处从外部全面反射入射到传感器芯片上的光，并观察全反射光的物理值。

公开(公告)号：US20060063984A1

公开(公告)日：2006-03-23

申请号：US10899264

申请日：2004-07-26

申请人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

发明人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

IPC分类号： A61B5/00

CPC分类号： A61B5/14514 ,  A61B5/14532 ,  A61B5/1455

摘要： An optical waveguide type iontophoresis sensor chip has a substrate having a first hinged plate and a second hinged plate, an optical waveguide plate connected to the first hinged plate and positioned over a first opening delineated in the first hinged plate, and a gel layer fixed on the second hinged plate and covering a second opening delineated in the second hinged plate and configured to contact with the optical waveguide plate through the first opening in case that the substrate is folded.

摘要翻译： 光波导型离子电渗传感器芯片具有：具有第一铰接板和第二铰接板的基板;连接到第一铰接板的光波导板，定位在第一铰接板中描绘的第一开口上;以及固定在 所述第二铰接板并且覆盖在所述第二铰接板中描绘的第二开口，并且构造成在所述基板被折叠的情况下通过所述第一开口与所述光波导板接触。

公开(公告)号：US07678567B2

公开(公告)日：2010-03-16

申请号：US10899266

申请日：2004-07-26

申请人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

发明人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C12Q1/00 ,  C12Q1/28 ,  C12Q1/54 ,  G01N21/7703 ,  G01N21/78

摘要： An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by the first and second gratings on the total reflection plate.

摘要翻译： 光学生物传感器具有全反射板，以全反射和透射入射光，第一光栅和第二光栅分别设置在全反射板上，以及传感膜，其包含酶和显色试剂，并且是 夹在全反射板上的第一和第二光栅。

公开(公告)号：US07410614B2

公开(公告)日：2008-08-12

申请号：US10899264

申请日：2004-07-26

申请人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

发明人： Kenichi Uchiyama ,  Ikuo Uematsu ,  Kayoko Oomiya ,  Ichiro Tono ,  Hideo Eto ,  Isao Kishimoto ,  Naotada Okada ,  Masami Hirata

IPC分类号： G01N21/00 ,  G02B6/10

CPC分类号： A61B5/14514 ,  A61B5/14532 ,  A61B5/1455

摘要： An optical waveguide type iontophoresis sensor chip has a substrate having a first hinged plate and a second hinged plate, an optical waveguide plate connected to the first hinged plate and positioned over a first opening delineated in the first hinged plate, and a gel layer fixed on the second hinged plate and covering a second opening delineated in the second hinged plate and configured to contact with the optical waveguide plate through the first opening in case that the substrate is folded.

摘要翻译： 光波导型离子电渗传感器芯片具有：具有第一铰接板和第二铰接板的基板;连接到第一铰接板的光波导板，定位在第一铰接板中描绘的第一开口上;以及固定在 所述第二铰接板并且覆盖在所述第二铰接板中描绘的第二开口，并且构造成在所述基板被折叠的情况下通过所述第一开口与所述光波导板接触。

公开(公告)号：US20060194345A1

公开(公告)日：2006-08-31

申请号：US11363094

申请日：2006-02-28

申请人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

发明人： Kenichi Uchiyama ,  Kayoko Oomiya ,  Isao Kishimoto ,  Masami Hirata ,  Hideo Eto ,  Ichiro Tono ,  Ikuo Uematsu ,  Shingo Kasai ,  Tomohiro Takase ,  Tsutomu Honjoh ,  Masanori Sugitani

IPC分类号： G01N33/543 ,  C12M1/34

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： The method for measuring the concentration of the measuring object uses a sensor chip comprising an optical waveguide layer and an antibody immobilized layer formed on the surface of the optical waveguide layer, which comprises immobilizing the measuring object and an enzyme-labeled antibody labeled with a labeling enzyme on the antibody immobilized layer of the sensor chip having an immobilized antibody, producing a color-developing and precipitating enzyme reaction product by allowing to react a coloring reagent with the labeling enzyme on the antibody immobilized layer to precipitate the enzyme reaction product on the antibody immobilized layer, allowing to totally reflect a light impinged on the sensor chip from the outside at an interface between the optical waveguide layer and the antibody immobilized layer, and observing to a physical value of the totally reflected light.

公开(公告)号：US20060019374A1

公开(公告)日：2006-01-26

申请号：US10899266

申请日：2004-07-26

申请人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

发明人： Ikuo Uematsu ,  Ichiro Tono ,  Kayoko Oomiya ,  Hideo Eto

IPC分类号： C12M1/34

CPC分类号： C12Q1/00 ,  C12Q1/28 ,  C12Q1/54 ,  G01N21/7703 ,  G01N21/78

摘要： An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by the first and second gratings on the total reflection plate.

摘要翻译： 光学生物传感器具有全反射板，以全反射和透射入射光，第一光栅和第二光栅分别设置在全反射板上，以及传感膜，其包含酶和显色试剂，并且是 夹在全反射板上的第一和第二光栅。

公开(公告)号：US20110180725A1

公开(公告)日：2011-07-28

申请号：US13080274

申请日：2011-04-05

申请人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

发明人： Kenichi Uchiyama ,  Shingo Kasai ,  Ikuo Uematsu ,  Tomohiro Takase ,  Kayoko Oomiya ,  Ichiro Tono ,  Tetsuo Sekino ,  Seiichirou Ishioka ,  Takashi Kawamura

IPC分类号： G01J1/58

CPC分类号： G01N21/6428 ,  G01N21/07 ,  G01N21/645

摘要： A fluorometric apparatus capable of configuring to have a small size and operability requiring a simple and easy operation of centrifuging a specimen in a container a rotation center of which is detachably supported by a rotation axis (not shown), exciting the specimen by means of an N2 laser apparatus and an excitation light irradiator for guiding the laser light emitted from the N2 laser apparatus, and receiving fluorescence having two types of wavelengths emitted from antibodies labeled with fluorescent dyes in the specimen in a region separate from the region in which the excitation light is radiated by a predetermined angle by using a photomultiplier tube, obtaining a ratio of light intensity for each wavelength, comparing the obtained ratio with a standard value, and estimating a target protein concentration.

摘要翻译： 一种能够构造成具有小尺寸和可操作性的荧光测定装置，其需要简单且容易地将样品离心在容器中，旋转中心由旋转轴线（未示出）可拆卸地支撑，借助于 N2激光装置和激发光照射器，用于引导从N2激光装置发射的激光，并且在与激发光的区域分开的区域中接收在样品中的荧光染料标记的抗体的两种类型的波长的荧光 通过使用光电倍增管照射预定角度，获得每个波长的光强比，将获得的比值与标准值进行比较，并估计目标蛋白质浓度。

公开(公告)号：US20090124024A1

公开(公告)日：2009-05-14

申请号：US12266023

申请日：2008-11-06

申请人： Shingo Kasai ,  Ikuo Uematsu ,  Ichiro Tono ,  Tomohiro Takase ,  Isao Nawata ,  Kayoko Oomiya ,  Yuriko Oyama ,  Tsutomu Honjoh

发明人： Shingo Kasai ,  Ikuo Uematsu ,  Ichiro Tono ,  Tomohiro Takase ,  Isao Nawata ,  Kayoko Oomiya ,  Yuriko Oyama ,  Tsutomu Honjoh

IPC分类号： G01N33/543 ,  G01N21/00 ,  B05D5/06

CPC分类号： G01N21/75 ,  G01N21/552 ,  G01N21/7703 ,  G01N33/54313 ,  G01N33/54373 ,  G02B6/12007 ,  G02B6/124

摘要： An optical-waveguide sensor chip includes an optical waveguide having a first substance immobilized on the surface thereof, the first substance being specifically reactive with an analyte substance, and fine particles dispersed on the optical waveguide and having a second substance immobilized on the surface thereof, the second substance being specifically reactive with the analyte substance.

摘要翻译： 光波导传感器芯片包括具有固定在其表面上的第一物质的光波导，第一物质与分析物质特异性反应，以及分散在光波导上并且固定在其表面上的第二物质的细颗粒， 第二物质与分析物质特异性反应。

公开(公告)号：US20070081758A1

公开(公告)日：2007-04-12

申请号：US11393771

申请日：2006-03-31

申请人： Ichiro Tono ,  Kayoko Oomiya ,  Tomohiro Takase ,  Ikuo Uematsu

发明人： Ichiro Tono ,  Kayoko Oomiya ,  Tomohiro Takase ,  Ikuo Uematsu

IPC分类号： G02B6/00

CPC分类号： G02B6/124 ,  G01N21/7703 ,  G01N21/78 ,  G01N2021/7709 ,  G01N2021/775 ,  G01N2021/7783

摘要： An optical waveguide type biochemical sensor chip includes a light beam transmittable substrate having at least a first optical element that allows light beam to be impinged to the inside and a second optical element that emits light beam from the inside, an optical waveguide layer that is formed on a main surface of the substrate on which at least one of the first and second optical elements is formed, has a thickness of 3 to 300 μm and is made of a polymer resin material having a higher refractive index than that of the substrate material, and a sensing membrane that is formed on the optical waveguide layer and creates a reaction product having the ability of absorbing the light beam or an evanescent wave of the light beam in response to an introduced specimen.