公开(公告)号：US20250002974A1

公开(公告)日：2025-01-02

申请号：US18758066

申请日：2024-06-28

Applicant: Illumina, Inc.

Inventor： Sherman Lauw ,  Trina Osothprarop ,  Henry Day ,  Johan Basuki ,  Claire Bevis-Mott ,  Hester Beard ,  Chol Steven Yun ,  Faye Huber ,  Patrizia Iavicoli ,  Yin Nah Teo ,  Mei Xian Agnes Lee ,  Denise Ng

IPC: C12Q1/6806 ,  C12Q1/6874

Abstract: This application relates to methods and compositions for protecting fully functional nucleotides (ffNs) against degradation. In some examples, micelles or surfactants are used to protect ffNs. In some examples, bulky cation compounds are used to protect ffNs. In some examples, the compositions that protect ffNs are included in lyophilized material.

公开(公告)号：US20240318161A1

公开(公告)日：2024-09-26

申请号：US18613690

申请日：2024-03-22

Applicant: ILLUMINA, INC.

Inventor： Vicki Thomson ,  Maria Vitoriano ,  Sébastien Ricoult ,  Johan Basuki

IPC: C12N15/10 ,  C40B50/00

CPC classification number: C12N15/1003 ,  C40B50/00

Abstract: The present disclosure provides systems and methods for preparing a DNA library from a sample comprising shelf-stable lyophilized microspheres comprising DNA library preparation reagents providing a streamlined workflow for DNA library preparation. The present disclosure also provides a smart consumable container for collecting and transporting the sample.

公开(公告)号：US20240182959A1

公开(公告)日：2024-06-06

申请号：US18515013

申请日：2023-11-20

Applicant: Illumina, Inc.

Inventor： Hassan Bohra ,  Johan Basuki ,  Wei Wei ,  Brian Mather

IPC: C12Q1/6855 ,  B01L3/00 ,  C12Q1/6806

CPC classification number: C12Q1/6855 ,  B01L3/508 ,  C12Q1/6806 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0809

Abstract: Some examples herein provide a hydrogel on a substrate. The hydrogel includes a three-dimensional network of polymer chains; first functional groups coupled to the polymer chains; amplification primers coupled to the polymer chains via the first functional groups; and second functional groups coupled to the polymer chains and reversibly cross-linking the polymer chains to one another. Some examples herein provide a method of using a hydrogel. The method includes hybridizing a target polynucleotide to an amplification primer coupled to a hydrogel; cleaving cross-linkages within the hydrogel within which the target polynucleotide is hybridized to the amplification primer; and amplifying the target polynucleotide using additional amplification primers within the hydrogel within which the cross-linkages have been cleaved.

公开(公告)号：US20240150508A1

公开(公告)日：2024-05-09

申请号：US18482417

申请日：2023-10-06

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited ,  Illumina Singapore Pte. Ltd.

Inventor： Nam Nguyen ,  Xavier von Hatten ,  Wayne George ,  Gianluca Artioli ,  Brian Mather ,  Johan Basuki ,  Shima Gholizadeh

IPC: C08F220/56 ,  C08J3/24 ,  C08J3/28

CPC classification number: C08F220/56 ,  C08J3/245 ,  C08J3/28 ,  C08F2810/20 ,  C08F2810/40 ,  C08J2333/26

Abstract: In some examples, novel photochemically-reversible hydrogels and nanogel particles are described comprising copolymer chains including at least one reactive alkene or reactive 1,4-diene end group capable of [2+2] or [2+2+2+2] photodimerization, respectively, at wavelengths >270 nm. In various examples, the photochemically-reversible hydrogels comprise copolymer chains including at least one —N3, —C≡CH or —CO2H end group for dual functionality and/or pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to photochemically-reversible hydrogels or nanogel particles reversibly bound to surfaces within a flow cell. After sequencing is complete, the photochemically-reversible hydrogel or nanogel particles is/are removable from the flow cell surfaces by irradiation, enabling the flow cell to be reusable.