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    MODIFIED NUCLEOTIDES
    3.
    发明申请
    MODIFIED NUCLEOTIDES 审中-公开

    公开(公告)号：US20200017908A1

    公开(公告)日：2020-01-16

    申请号：US16523810

    申请日：2019-07-26

    申请人： Illumina Cambridge Limited

    发明人： John MILTON ,  Xiaolin WU ,  Mark Edward Brennan SMITH ,  Joseph BRENNAN ,  Colin Lloyd BARNES ,  Xiaohai LIU ,  Silke RUEDIGER

    IPC分类号： C12Q1/6869 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H1/00 ,  C07H19/14

    摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R′)2-O—R″, —C(R′)2-N(R″)2, —C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula=C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H.

    MODIFIED NUCLEOTIDES
    6.
    发明申请
    MODIFIED NUCLEOTIDES 审中-公开
    改性核酸

    公开(公告)号：US20160362737A1

    公开(公告)日：2016-12-15

    申请号：US15179813

    申请日：2016-06-10

    申请人： Illumina Cambridge Limited

    发明人： John MILTON ,  Xiaolin WU ,  Mark SMITH ,  Joseph BRENNAN ,  Colin BARNES ,  Xiaohai LIU ,  Silke RUEDIGER

    IPC分类号： C12Q1/68 ,  C07H19/14 ,  C07H19/10

    CPC分类号： C12Q1/6869 ,  C07H1/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q2525/186 ,  C12Q2535/113 ,  Y02P20/55 ,  C12Q2525/197

    摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R′)2—O—R″, —C(R′)2-N(R″)2,—C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula ═C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H.

    摘要翻译： 本发明提供了修饰的核苷酸或核苷分子，其包含嘌呤或嘧啶碱基和核糖或脱氧核糖糖部分，其具有共价连接到其上的可移除的3'-OH封闭基团，使得3'碳原子连接一组结构-O -Z其中Z是-C（R'）2 -O-R“，-C（R'）2 -N（R”）2，-C（R'）2 -N（H）R“ -C（R'）2 -S-R“和-C（R'）2 -F，其中每个R”为或可移除的保护基团的一部分; 每个R'独立地是氢原子，烷基，取代的烷基，芳基烷基，烯基，炔基，芳基，杂芳基，杂环，酰基，氰基，烷氧基，芳氧基，杂芳氧基或酰氨基，或通过连接基团连接的可检测标记; 或（R'）2表示式≡C（R'“）2的亚烷基，其中每个R”可以相同或不同，并且选自氢和卤素原子和烷基; 并且其中所述分子可以反应以产生其中每个R“被交换为H的中间体，或其中Z是-C（R'）2 -F，F被交换成OH，SH或NH 2，优选OH，其中 中间体在水性条件下解离得到具有游离3'OH的分子; 条件是其中Z是-C（R'）2 -S-R“，两个R'基团不是H.

    LABELLED NUCLEOTIDES
    9.
    发明申请
    LABELLED NUCLEOTIDES 有权
    标签核心

    公开(公告)号：US20150344516A1

    公开(公告)日：2015-12-03

    申请号：US14821592

    申请日：2015-08-07

    申请人： Illumina Cambridge Limited

    发明人： Xiaohai LIU ,  John MILTON ,  Silke RUEDIGER

    IPC分类号： C07H19/10 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q1/6874 ,  Y10T436/143333 ,  C12Q2535/113 ,  C12Q2525/186

    摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).

    摘要翻译： 本发明提供了具有通过可切割接头连接到可检测标记的碱基的核苷酸或核苷，其特征在于所述可切割连接体含有选自以下的部分：式（I）（其中X选自O， S，NH和NQ，其中Q是C 1-10取代或未取代的烷基，Y选自O，S，NH和N（烯丙基），T是氢或C 1-10取代或未取代的烷基， *表示部分连接到核苷酸或核苷的剩余部分）。

    LABELLED NUCLEOTIDES
    10.
    发明申请
    LABELLED NUCLEOTIDES 有权
    标签核心

    公开(公告)号：US20140141414A1

    公开(公告)日：2014-05-22

    申请号：US14073593

    申请日：2013-11-06

    申请人： ILLUMINA CAMBRIDGE LIMITED

    发明人： Xiaohai LIU ,  John MILTON ,  Silke RUEDIGER

    IPC分类号： C12Q1/68

    CPC分类号： C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/14 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q1/6874 ,  Y10T436/143333 ,  C12Q2535/113 ,  C12Q2525/186

    摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (1) (wherein X is selected from the group comprising O, S, NH, and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH, and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).

    摘要翻译： 本发明提供了具有通过可切割接头与可检测标记连接的碱基的核苷酸或核苷，其特征在于所述可切割连接体含有选自以下的部分：式（1）（其中X选自O， S，NH和NQ，其中Q是C 1-10取代或未取代的烷基，Y选自O，S，NH和N（烯丙基），T是氢或C 1-10取代或未取代的烷基 基团和*表示部分连接到核苷酸或核苷的剩余部分）。