公开(公告)号：US20110236935A1

公开(公告)日：2011-09-29

申请号：US12915853

申请日：2010-10-29

申请人： Jørn Dalgaard Mikkelsen ,  Karsten Matthias Kragh ,  Rene Mikkelsen ,  Patrick Maria Franciscus Derkx ,  Shukun Yu ,  Harm Mulder ,  Igor Nikolaev

发明人： Jørn Dalgaard Mikkelsen ,  Karsten Matthias Kragh ,  Rene Mikkelsen ,  Patrick Maria Franciscus Derkx ,  Shukun Yu ,  Harm Mulder ,  Igor Nikolaev

IPC分类号： C12P19/04 ,  C12P19/00 ,  C12P19/12 ,  C12P19/10 ,  C08B37/16 ,  C08B31/18 ,  C08B37/00 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19

CPC分类号： C12N11/14 ,  C12N9/0006 ,  C12P19/00

摘要： A method of oxidising a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidised saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidised saccharide, are also described.

摘要翻译： 通过使糖与选自醌氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸（NAD +）氧化还原辅因子依赖性ADH，烟酰胺腺嘌呤二核苷酸磷酸（NADP +）的NADP +）的醇脱氢酶（ADH）酶接触来进行氧化糖的方法， 描述了氧化还原辅因子依赖性ADH及其任何组合。 还描述了通过该方法获得的氧化糖，特别是含有氧化糖的食品和纸制品。

公开(公告)号：US08802398B2

公开(公告)日：2014-08-12

申请号：US12915853

申请日：2010-10-29

申请人： Jorn Dalgaard Mikkelsen ,  Karsten Matthias Kragh ,  Rene Mikkelsen ,  Patrick Maria Franciscus Derkx ,  Shukun Yu ,  Harm Mulder ,  Igor Nikolaev

发明人： Jorn Dalgaard Mikkelsen ,  Karsten Matthias Kragh ,  Rene Mikkelsen ,  Patrick Maria Franciscus Derkx ,  Shukun Yu ,  Harm Mulder ,  Igor Nikolaev

IPC分类号： C12P19/04 ,  C12P19/10 ,  C12P19/00 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N11/14 ,  C12N9/0006 ,  C12P19/00

摘要： A method of oxidizing a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidized saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidized saccharide, are also described.

摘要翻译： 通过使糖类与选自醌氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸（NAD +）氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸磷酸（NADP +））的醇脱氢酶（ADH）酶接触来进行氧化糖的方法， 描述了氧化还原辅因子依赖性ADH及其任何组合。 还描述了通过该方法获得的氧化糖，特别是含有氧化糖的食品和纸制品。

公开(公告)号：US06300103B1

公开(公告)日：2001-10-09

申请号：US09488200

申请日：2000-01-19

申请人： Karsten Matthias Kragh ,  Jørn Dalgaard Mikkelsen ,  Klaus Kristian Nielsen

发明人： Karsten Matthias Kragh ,  Jørn Dalgaard Mikkelsen ,  Klaus Kristian Nielsen

IPC分类号： C07H2104

CPC分类号： C12N15/8282 ,  A01N65/08 ,  C07K14/415

摘要： An anti-microbial protein comprising a peptide having the amino acid sequence: AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-Cys-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18-AA19-AA20-AA21-Cys-AA23-AA24-AA25-AA26-AA27-AA28-Cys-AA30. An anti-microbial protein having the sequence depicted in any one of SEQ ID Nos 1-3. Recombinant DNA encoding such proteins. A vector comprising such DNA which is expressible in plants and which is linked to a plant operable promoter and terminator. Plants transformed with such recombinant DNA; the progeny of such plants which contain the DNA stably incorporated and hereditable in a Mendelian manner, and/or the seeds of such plants or such progeny.

摘要翻译： 包含具有氨基酸序列的肽的抗微生物蛋白质：AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-Cys-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18- AA19-AA20-AA21-Cys-AA23-AA24-AA25-AA26-AA27-AA28-Cys-AA30。 具有SEQ ID No.3-1中任一项所示序列的抗微生物蛋白质。 编码这种蛋白质的重组DNA。 包含这样的DNA的载体，其在植物中可表达并与植物可操作的启动子和终止子连接。 用这种重组DNA转化的植物; 这些植物的后代含有以孟德尔方式稳定掺入和遗传的DNA，和/或这种植物或其后代的种子。

公开(公告)号：US06218508B1

公开(公告)日：2001-04-17

申请号：US08632511

申请日：1996-04-17

申请人： Karsten Matthias Kragh ,  Jørn Dalgaard Mikkelsen ,  Klaus Kristian Nielsen

发明人： Karsten Matthias Kragh ,  Jørn Dalgaard Mikkelsen ,  Klaus Kristian Nielsen

IPC分类号： C07K500

CPC分类号： C12N15/8282 ,  A01N65/08 ,  C07K14/415

摘要： An anti-microbial protein comprising a peptide having the amino acid sequence: AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-CYS-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18-AA19-AA20-AA21-Cys-AA23-AA24-AA25-AA26-AA27-AA28-Cys-AA30. An anti-microbial protein having the sequence depicted in any one of SEQ ID Nos 1-3. Recombinant DNA encoding such proteins. A vector comprising such DNA which is expressible in plants and which is linked to a plant operable promoter and terminator. Plants transformed with such recombinant DNA; the progeny of such plants which contain the DNA stably incorporated and hereditable in a Mendelian manner, and/or the seeds of such plants or such progeny.

摘要翻译： 包含具有氨基酸序列的肽的抗微生物蛋白质：AA1-AA2-AA3-Cys-AA5-AA6-AA7-AA8-AA9-CYS-AA11-AA12-AA13-AA14-Cys-Cys-AA17-AA18- AA19-AA20-AA21-Cys-AA23-AA24-AA25-AA26-AA27-AA28-Cys-AA30。 具有SEQ ID No.3-1中任一项所示序列的抗微生物蛋白质。 编码这种蛋白质的重组DNA。 包含这样的DNA的载体，其在植物中可表达并与植物可操作的启动子和终止子连接。 用这种重组DNA转化的植物; 这些植物的后代含有以孟德尔方式稳定掺入和遗传的DNA，和/或这种植物或其后代的种子。

公开(公告)号：US20080227173A1

公开(公告)日：2008-09-18

申请号：US11534624

申请日：2006-09-22

申请人： Casper Tune BERG ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune BERG ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

IPC分类号： C12N9/28

CPC分类号： A21D8/042 ,  A23K20/147 ,  A23K50/30 ,  A23K50/75 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。

公开(公告)号：US08030050B2

公开(公告)日：2011-10-04

申请号：US11534624

申请日：2006-09-22

申请人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sørensen ,  Charlotte Refdahl Thoudahl

IPC分类号： C12N9/28 ,  C12N9/26 ,  C12N9/24 ,  C12N9/00 ,  C12P21/06 ,  A21D2/00 ,  A23C9/12 ,  C07H21/04

CPC分类号： A21D8/042 ,  A23K20/147 ,  A23K50/30 ,  A23K50/75 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。

公开(公告)号：US20080292747A1

公开(公告)日：2008-11-27

申请号：US11970473

申请日：2008-01-07

申请人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

发明人： Casper Tune Berg ,  Patrick Maria Franciscus Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Hemmingen Kellet-Smith ,  Karsten Matthias Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Spange Sorensen ,  Charlotte Refdahl Thoudahl

IPC分类号： A21D2/08 ,  A21D13/06 ,  C12N9/28 ,  C12P19/00

CPC分类号： A21D8/042 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： Described is a PS4 variant polypeptide derivable from a polypeptide having amylase activity selected from: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; referring to the numbering of a Pseudomonas saccharophilia exoamylase shown as SEQ ID NO: 1.

摘要翻译： 描述了可衍生自具有选自以下的淀粉酶活性的多肽的PS4变体多肽： 223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 指的是SEQ ID NO：1所示的假单胞菌糖酵母外切酶的编号。

公开(公告)号：US08178336B2

公开(公告)日：2012-05-15

申请号：US12339718

申请日：2008-12-19

申请人： Patrick Maria Franciscus Derkx ,  Anja Kellet-Smith Hemmingen ,  Rie Mejldal ,  Bo Spange Sørensen ,  Karsten Matthias Kragh

发明人： Patrick Maria Franciscus Derkx ,  Anja Kellet-Smith Hemmingen ,  Rie Mejldal ,  Bo Spange Sørensen ,  Karsten Matthias Kragh

IPC分类号： C12N9/28 ,  C12N9/24 ,  C12N9/00 ,  C12P21/06 ,  C07H21/04 ,  A21D2/00 ,  A23C9/12

CPC分类号： C12N9/2417 ,  C12N9/2411 ,  C12N9/2414

摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).

摘要翻译： 我们描述了可从具有非麦芽糖外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽包含307位氨基酸取代赖氨酸（K）或精氨酸（R），参考位置编号 优选地，PS4变体多肽还包含70位氨基酸取代，优选G70D。 序列272和303位氨基酸优选为组氨酸（H）和甘氨酸（G）。

公开(公告)号：US20090202675A1

公开(公告)日：2009-08-13

申请号：US12339718

申请日：2008-12-19

申请人： Patrick Maria Franciscus Derkx ,  Anja Kellet-Smith Hemmingen ,  Rie Mejldal ,  Bo Spange Sorensen ,  Karsten Matthias Kragh

发明人： Patrick Maria Franciscus Derkx ,  Anja Kellet-Smith Hemmingen ,  Rie Mejldal ,  Bo Spange Sorensen ,  Karsten Matthias Kragh

IPC分类号： A21D2/26 ,  C12N9/28 ,  C12P19/00 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21 ,  C12N1/19 ,  C12P21/02 ,  A23L1/105 ,  A23K1/165 ,  A21D13/00

CPC分类号： C12N9/2417 ,  C12N9/2411 ,  C12N9/2414

摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).

摘要翻译： 我们描述了可从具有非麦芽糖外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽包含307位氨基酸取代赖氨酸（K）或精氨酸（R），参考位置编号 优选地，PS4变体多肽还包含70位氨基酸取代，优选G70D。 序列272和303位氨基酸优选为组氨酸（H）和甘氨酸（G）。

公开(公告)号：US07858352B2

公开(公告)日：2010-12-28

申请号：US11635511

申请日：2006-12-08

申请人： Karsten Matthias Kragh ,  Harm Mulder ,  Steffen Petersen ,  Helle Fomsgaard ,  Oene Robert Veltman

发明人： Karsten Matthias Kragh ,  Harm Mulder ,  Steffen Petersen ,  Helle Fomsgaard ,  Oene Robert Veltman

IPC分类号： C12N9/28 ,  C12N15/00 ,  C12N9/88

CPC分类号： A21D8/042 ,  A23K20/189 ,  A23L29/35 ,  C12N9/2417 ,  C12Y302/0106

摘要： We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.

摘要翻译： 我们公开了可从母体多肽衍生的PS4变体多肽，该亲本多肽具有非产麦芽糖外切酶活性，该PS4变体多肽包含一个或多个以下取代：G69P，A141P，G223A，A268P，G313P，S399P和G400P，具有 提及如SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列的位置编号。这样的PS4变体多肽可以用作外切淀粉酶，特别是作为非发酵的外消旋酶。 公开了这些PS4变体多肽与Novamyl的组合。