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    Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation
    1.
    发明申请
    Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation 有权
    用于生存和不可行的原核和真核细胞定量的方法和装置

    公开(公告)号：US20050282244A1

    公开(公告)日：2005-12-22

    申请号：US11144244

    申请日：2005-06-02

    Applicant: James Fleming ,  Jason Somes ,  Darby Mclean ,  Jerad Holcomb

    Inventor： James Fleming ,  Jason Somes ,  Darby Mclean ,  Jerad Holcomb

    IPC: C12Q1/06 ,  C12Q1/04 ,  C12Q1/34

    CPC classification number: C12Q1/06 ,  Y10T436/101666

    Abstract: A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound, 2) isolating the washed cells by centrifugation or filtration, 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells, 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer, and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.

    Abstract translation: 描述了用于定量各种活细胞类型的快速方法。 该方法可以包括多种步骤，包括：1）将细胞悬浮在洗涤剂状化合物中，2）通过离心或过滤分离洗涤的细胞; 3）将细胞重新悬浮于含有防腐剂，荧光染料和 可以被细胞吸收的脱金属化合物，4）用简单的荧光计测量细胞染料混合物随时间的荧光增加，和5）测量细胞的天然荧光。 这种新的细胞荧光方法与其他枚举细胞的方法相关，例如标准平板计数，亚甲基蓝法和载玻片活力技术。 该方法在以下几个应用中特别有用：a）定量牛奶，酸奶，奶酪，肉类和其他食物中的细菌，b）定量酿造，发酵和面包制作中的酵母细胞，c）定量研究，食品和 临床设置。 当需要总细胞计数和活细胞计数时，如在酿造工业中，该方法特别有用。 该方法也可用于确定样品中细胞的代谢活性。 还公开了用于细胞定量的装置，装置和/或系统。

    Methods, compositions, devices, and kits for detecting mastitis
    2.
    发明申请
    Methods, compositions, devices, and kits for detecting mastitis 审中-公开
    用于检测乳腺炎的方法，组合物，装置和试剂盒

    公开(公告)号：US20050260695A1

    公开(公告)日：2005-11-24

    申请号：US10948076

    申请日：2004-09-23

    Applicant: James Fleming ,  Jason Somes ,  Stephanie Bernards ,  Tawyna St. Pierre

    Inventor： James Fleming ,  Jason Somes ,  Stephanie Bernards ,  Tawyna St. Pierre

    IPC: C12M1/34 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/558 ,  G01N33/569

    CPC classification number: G01N33/558 ,  G01N33/56938 ,  G01N2333/31 ,  G01N2333/79

    Abstract: The present invention includes compositions, kits and methods useful for the detection of mastitis in an animal. These agents and methods are primarily directed to a method of detecting the presence of mastitis, including sub-clinical mastitis, in cows, involving incubating a sample of milk from the cow with an agent that binds to lactoferrin such as, e.g., a monoclonal antibody specific for lactoferrin, and then detecting bound lactoferrin. The invention includes lateral-flow immunoassay methods and devices for assessing the presence of lactoferrin in milk samples.

    Abstract translation: 本发明包括可用于检测动物乳腺炎的组合物，试剂盒和方法。 这些试剂和方法主要涉及一种检测奶牛中乳腺炎（包括亚临床乳腺炎）的存在的方法，包括用牛血清蛋白（例如单克隆抗体）将来自母牛的乳样品孵育 特异性用于乳铁蛋白，然后检测结合的乳铁蛋白。 本发明包括用于评估乳样品中乳铁蛋白的存在的侧流免疫测定方法和装置。

    Method of fabricating optical fiber using an isothermal, low pressure plasma deposition technique
    4.
    发明授权
    Method of fabricating optical fiber using an isothermal, low pressure plasma deposition technique 有权
    使用等温，低压等离子体沉积技术制造光纤的方法

    公开(公告)号：US08857372B2

    公开(公告)日：2014-10-14

    申请号：US12890983

    申请日：2010-09-27

    Applicant: James Fleming ,  George Zydzik

    Inventor： James Fleming ,  George Zydzik

    IPC: B05D5/06 ,  B05D7/22 ,  B05D3/06 ,  C03B37/01 ,  C03B37/018 ,  C03B37/022

    CPC classification number: C03B37/0183

    Abstract: An isothermal, low pressure-based process of depositing material within a substrate has been developed and results in creating an extremely narrow reaction zone within which a more uniform and efficient deposition will occur. Sets of isothermal plasma operating conditions have been found that create a narrow deposition zone, assuring that the deposited material is clear glass rather than soot particles. The chemical delivery system, in one arrangement, utilizes rods of solid phase source material (which may otherwise be difficult to obtain in gaseous form). The operating conditions are selected such that the hot plasma does not transfer a substantial amount of heat to the substrate tube, where the presence of such heat has been found to result in vaporizing the reactant material (creating soot) and developing hot spots.

    Abstract translation: 已经开发了在衬底内沉积材料的基于等温，低压的等离子体方法，并且导致产生非常窄的反应区域，在该区域内将发生更均匀和有效的沉积。 已经发现了等温等离子体操作条件的集合，其产生了窄的沉积区域，确保沉积的材料是透明玻璃而不是烟灰颗粒。 在一种布置中，化学输送系统利用固相源材料棒（否则可能难以以气体形式获得）。 选择操作条件使得热等离子体不向衬底管传送大量的热量，其中已经发现存在这种热导致反应物材料（产生烟灰）和形成热点的蒸发。

    Pilot's flight desk
    7.
    发明授权
    Pilot's flight desk 失效
    飞行员的飞行台

    公开(公告)号：US5542360A

    公开(公告)日：1996-08-06

    申请号：US913769

    申请日：1992-06-12

    Applicant: James Fleming

    Inventor： James Fleming

    IPC: A47B23/04 ,  A47C7/70 ,  B60N3/00 ,  B64D11/06

    CPC classification number: B64D11/0689 ,  A47B23/04 ,  A47C7/70 ,  B60N3/002

    Abstract: A pilot's flight desk for use in aircraft as disclosed. The flight desk provides a planar desk surface supported above the aircraft floor by an upright support attached leg to a base plate which is clamped to the aircraft seat rails. The desk surface swivels and pivots with respect to the upright support and the upright support is pivotally attached to the base plate, thereby allowing for the compact folding of the pilot flight desk. A cup holder and swivel mounted pen light attached to the desk surface is also disclosed.

    Abstract translation: 一名飞行员的飞行服务台，用于飞机上。 飞行台提供了一个平面的桌面，支撑在飞机地板之上，一个直立支撑将附接的腿连接到夹在飞机座椅导轨上的基板上。 桌面相对于直立支架旋转并枢转，并且直立支撑件枢转地附接到基板，从而允许飞行员飞行台的紧凑折叠。 还公开了连接到桌面的杯架和旋转安装的笔灯。

    Associative matrix methods, systems and computer program products using bit plane representations of selected segments
    10.
    发明申请
    Associative matrix methods, systems and computer program products using bit plane representations of selected segments 有权
    使用所选段的位平面表示的关联矩阵方法，系统和计算机程序产品

    公开(公告)号：US20070033346A1

    公开(公告)日：2007-02-08

    申请号：US11196871

    申请日：2005-08-04

    Applicant: Michael Lemen ,  James Fleming ,  Manuel Aparicio

    Inventor： Michael Lemen ,  James Fleming ,  Manuel Aparicio

    IPC: G06F12/00

    CPC classification number: G06K9/6247 ,  G06N3/0445 ,  G06N3/063

    Abstract: Associative matrix compression methods, systems, computer program products and data structures compress an association matrix that contains counts that indicate associations among pairs of attributes. Selective bit plane representations of those selected segments of the association matrix that have at least one count is performed, to allow compression. More specifically, a set of segments is generated, a respective one of which defines a subset, greater than one, of the pairs of attributes. Selective identifications of those segments that have at least one count are stored. The at least one count that is associated with a respective identified segment is also stored as at least one bit plane representation. The at least one bit plane representation identifies a value of the at least one associated count for a bit position of the count that corresponds to the associated bit plane.

    Abstract translation: 关联矩阵压缩方法，系统，计算机程序产品和数据结构压缩包含指示属性对之间关联的计数的关联矩阵。 执行具有至少一个计数的关联矩阵的那些所选段的选择性位平面表示，以允许压缩。 更具体地，生成一组片段，其中相应的片段定义了属性对中大于一个的子集。 存储具有至少一个计数的那些段的选择性标识。 与相应识别的段相关联的至少一个计数也被存储为至少一个位平面表示。 所述至少一个位平面表示标识对应于相关联的位平面的计数的位位置的至少一个关联计数的值。

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