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1.发明授权DNA and RNA sequencing by nanoscale reading through programmable electrophoresis and nanoelectrode-gated tunneling and dielectric detection 有权通过可编程电泳和纳米电极门控隧道和电介质检测的纳米级读数进行DNA和RNA测序公开(公告)号：US06905586B2
公开(公告)日：2005-06-14
申请号：US10055881
申请日：2002-01-28
申请人： James W. Lee , Thomas G. Thundat
发明人： James W. Lee , Thomas G. Thundat
IPC分类号： B01L3/00 , C12Q1/68 , G01N27/447 , G01N27/26
CPC分类号： C12Q1/6869 , B01L3/502707 , B01L3/502761 , B01L2200/0647 , B01L2300/0645 , B01L2300/0896 , B01L2400/0421 , B82Y30/00 , C12Q2565/631 , C12Q2565/629 , C12Q2565/601
摘要： An apparatus and method for performing nucleic acid (DNA and/or RNA) sequencing on a single molecule. The genetic sequence information is obtained by probing through a DNA or RNA molecule base by base at nanometer scale as though looking through a strip of movie film. This DNA sequencing nanotechnology has the theoretical capability of performing DNA sequencing at a maximal rate of about 1,000,000 bases per second. This enhanced performance is made possible by a series of innovations including: novel applications of a fine-tuned nanometer gap for passage of a single DNA or RNA molecule; thin layer microfluidics for sample loading and delivery; and programmable electric fields for precise control of DNA or RNA movement. Detection methods include nanoelectrode-gated tunneling current measurements, dielectric molecular characterization, and atomic force microscopy/electrostatic force microscopy (AFM/EFM) probing for nanoscale reading of the nucleic acid sequences.
摘要翻译： 用于在单个分子上进行核酸（DNA和/或RNA）测序的装置和方法。 遗传序列信息是通过以纳米级的方式通过DNA或RNA分子碱基进行探索来获得的，就像看通过电影胶片一样。 该DNA测序纳米技术具有进行DNA测序的理论能力，最大速率约为每秒100万个碱基。 这种增强的性能可以通过一系列创新成为可能，包括：用于单个DNA或RNA分子通过的微调纳米间隙的新颖应用; 用于样品装载和分送的薄层微流体; 和可编程电场，用于精确控制DNA或RNA的运动。 检测方法包括纳米电极栅极隧道电流测量，电介质分子表征和用于核酸序列的纳米级读数的原子力显微镜/静电力显微镜（AFM / EFM）探测。
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2.发明授权公开(公告)号：US07033476B2
公开(公告)日：2006-04-25
申请号：US10335475
申请日：2002-12-31
申请人： James W. Lee , Thomas G. Thundat
发明人： James W. Lee , Thomas G. Thundat
IPC分类号： G01N27/453
CPC分类号： B01D57/02 , B82Y15/00 , C25D21/12 , G01N27/44791
摘要： An apparatus for carrying out the separation, detection, and/or counting of single molecules at nanometer scale. Molecular separation is achieved by driving single molecules through a microfluidic or nanofluidic medium using programmable and coordinated electric fields. In various embodiments, the fluidic medium is a strip of hydrophilic material on nonconductive hydrophobic surface, a trough produced by parallel strips of hydrophobic nonconductive material on a hydrophilic base, or a covered passageway produced by parallel strips of hydrophobic nonconductive material on a hydrophilic base together with a nonconductive cover on the parallel strips of hydrophobic nonconductive material. The molecules are detected and counted using nanoelectrode-gated electron tunneling methods, dielectric monitoring, and other methods.
摘要翻译： 用于以纳米级进行单分子的分离，检测和/或计数的装置。 通过使用可编程和协调的电场通过微流体或纳米流体介质驱动单分子来实现分子分离。 在各种实施例中，流体介质是在非导电疏水表面上的亲水材料条，在亲水基底上由疏水性非导电材料的平行条形成的槽，或由亲水基底上的平行疏水性非导电材料条形成的覆盖通道 在疏水非导电材料的平行条上具有不导电覆盖层。 使用纳米电极门控电子隧道法，电介质监测和其他方法检测和计数分子。
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公开(公告)号：US06231983B1
公开(公告)日：2001-05-15
申请号：US09181047
申请日：1998-10-27
申请人： Ida Lee , James W. Lee , Elias Greenbaum
发明人： Ida Lee , James W. Lee , Elias Greenbaum
IPC分类号： B32B1504
CPC分类号： H01L51/0595 , B82Y10/00 , Y10S428/91 , Y10T428/31504 , Y10T428/31678
摘要： Molecular devices using functional Photosystem I reaction centers are prepared on flat derivatized substrate surfaces. The nature and extent of orientation are controlled by chemical modification of the surface derivative. The surface of the substrate is chemically modified such that the Photosystem I reaction centers may be immobilized in a desired orientation. Preferably, a sulfur-organic compound is used to chemically modify the surface of the substrate. Depending on the type of chemical modification, the orientation of the reaction centers may be parallel to the surface, perpendicular to the surface in the “up” position, or perpendicular to the surface in the “down” position. The different orientations provide different electrical characteristics to the device.
摘要翻译： 使用功能性光系统I反应中心的分子装置在平坦衍生的基底表面上制备。 取向的性质和程度由表面衍生物的化学改性控制。 衬底的表面被化学修饰，使得光系统I反应中心可以以期望的取向固定。 优选地，使用硫 - 有机化合物来化学改性基材的表面。 取决于化学改性的类型，反应中心的取向可以平行于垂直于“向上”位置的表面或垂直于“向下”位置的表面的表面。 不同的方向为装置提供不同的电气特性。
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公开(公告)号：US08398738B2
公开(公告)日：2013-03-19
申请号：US12686831
申请日：2010-01-13
CPC分类号： C05F11/00 , C01B32/324 , C01B32/336
摘要： The invention is directed to a method for producing an oxygenated biochar material possessing a cation-exchanging property, wherein a biochar source is reacted with one or more oxygenating compounds in such a manner that the biochar source homogeneously acquires oxygen-containing cation-exchanging groups in an incomplete combustion process. The invention is also directed to oxygenated biochar compositions and soil formulations containing the oxygenated biochar material.
摘要翻译： 本发明涉及一种生产具有阳离子交换性能的含氧生物炭材料的方法，其中生物炭源与一种或多种氧化化合物反应，使得生物炭源均匀地获得含氧阳离子交换基团 不完全的燃烧过程。 本发明还涉及含氧生物炭组合物和含有氧化生物炭材料的土壤制剂。
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公开(公告)号：US20110172092A1
公开(公告)日：2011-07-14
申请号：US12686831
申请日：2010-01-13
IPC分类号： C01B31/08
CPC分类号： C05F11/00 , C01B32/324 , C01B32/336
摘要： The invention is directed to a method for producing an oxygenated biochar material possessing a cation-exchanging property, wherein a biochar source is reacted with one or more oxygenating compounds in such a manner that the biochar source homogeneously acquires oxygen-containing cation-exchanging groups in an incomplete combustion process. The invention is also directed to oxygenated biochar compositions and soil formulations containing the oxygenated biochar material.
摘要翻译： 本发明涉及一种生产具有阳离子交换性能的含氧生物炭材料的方法，其中生物炭源与一种或多种氧化化合物反应，使得生物炭源均匀地获得含氧阳离子交换基团 不完全的燃烧过程。 本发明还涉及含氧生物炭组合物和含有氧化生物炭材料的土壤制剂。
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公开(公告)号：US06318419B1
公开(公告)日：2001-11-20
申请号：US09796887
申请日：2001-03-02
申请人： James W. Lee
发明人： James W. Lee
IPC分类号： B65B104
CPC分类号： B65F1/10 , B65B67/1238 , B65F1/0006 , B65F2240/138
摘要： A collection system and method for various uses including the efficient collection of leaves. The system includes a specially designed chute element and an attachable draw-string type of plastic bag. The bag unit has plural perforations formed therein to provide for an equalization of air pressure and resultant ease of placement of collected items into the bag. The invention incorporates fluid mechanics principles related to air flow and air pressure equalization.
摘要翻译： 用于各种用途的收集系统和方法，包括有效收集叶子。 该系统包括专门设计的滑槽元件和可附接的拉绳式塑料袋。 袋单元具有形成在其中的多个穿孔，以提供空气压力的均衡，从而易于将收集的物品放置到袋中。 本发明结合了与气流和气压均衡相关的流体力学原理。
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公开(公告)号：US5090829A
公开(公告)日：1992-02-25
申请号：US642499
申请日：1991-01-17
申请人： James W. Lee , Michael A. Ditonto , Dale W. Roddy
发明人： James W. Lee , Michael A. Ditonto , Dale W. Roddy
CPC分类号： B41J19/202 , B41J29/38
摘要： A print head carriage of an impact printer is monitored by means of a segmented timing member which is separate from but coupled to and movable in coordination with the carriage. The segmented timing member is associated with a carriage drive motor and preferably comprises a toothed wheel which is rotated by a motor drive gear also used to move the print head carriage. The teeth of the toothed wheel are monitored by an optical sensor with a resulting series of pulses being accumulated to define the rotation of the toothed wheel and thereby the movement and position of the print head carriage. A home carriage position is directly indicated via a carriage position indicating member which is operated by the print head carriage to interrupt the optical sensor of the preferred embodiment.
摘要翻译： 冲击式打印机的打印头托架通过分段的定时构件来监测，该分段定时构件与托架分离但耦合到并与托架协调移动。 分段定时构件与托架驱动马达相关联，并且优选地包括由也用于移动打印头托架的马达驱动齿轮旋转的齿轮。 齿轮的齿由光学传感器监测，所产生的一系列脉冲被累积以限定齿轮的旋转，从而限定打印头滑架的移动和位置。 通过由打印头托架操作的托架位置指示构件直接指示家用托架位置，以中断优选实施例的光学传感器。
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公开(公告)号：US4015538A
公开(公告)日：1977-04-05
申请号：US580904
申请日：1975-05-27
申请人： Harry B. Webb , James W. Lee , Robert B. Blair
发明人： Harry B. Webb , James W. Lee , Robert B. Blair
摘要： An improved carcass transfer method and device are provided by which a single operator can readily raise, lower, and transport a whole beef carcass or other carcass to facilitate movement of the carcass both off of and onto overhead rail systems conventionally used for carcass transport and storage in meat packing plants. A portable cantilevered device having a lifting mechanism engages a roller-type trolley from which a carcass is suspended. The lifting mechanism preferably includes a hydraulic cylinder, which cylinder is in controlled communication with an oil reservoir. Pressurized air actuates various valves to effect a desired raising or lowering of the mast.
摘要翻译： 提供了一种改进的胎体转运方法和装置，通过该方法和装置，单个操作者可以容易地升高，降低和运输整个牛肉屠体或其他胎体，以便于胎体两侧离开和传播用于屠体运输和储存的架空轨道系统 在肉类包装厂。 具有提升机构的便携式悬臂装置与从其悬挂有胎体的辊式小车接合。 提升机构优选地包括液压缸，该液压缸与储油器控制连通。 加压空气致动各种阀门以实现所需的升高或降低桅杆。
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公开(公告)号：US5800080A
公开(公告)日：1998-09-01
申请号：US938791
申请日：1997-09-26
申请人： James W. Lee
发明人： James W. Lee
IPC分类号： B41J25/308 , B41J11/20
CPC分类号： B41J25/3082 , B41J25/308
摘要： A mechanism is provided with a slide adjustment member that is mounted on a frame supporting a carriage assembly of a printer. The carriage assembly is pivotally mounted upon a support shaft carried by a frame. The slide adjustment member has indexing teeth that engage with a plurality of saw-tooth detents. The sawtooth detents are longitudinally disposed upon a surface that is at a slight angle in the range between 3 and 4 degrees, and preferably about 3.44 degrees with respect to the horizontal. Horizontal movement of the slide member over this angled surface causes the carriage assembly to pivot about the support shaft. In so pivoting, the printhead carried upon the carriage assembly is forced to move with respect to the platen, thus changing the paper thickness gap therebetween. Each indexed movement of the slide member past a detent tooth provides a change of 0.003 inches in the gap. This change is equivalent to the difference between single and double ply paper. Thus, the invention provides a simple, low cost slide member and sawtooth index surface for changing the paper gap between the printhead and platen.
摘要翻译： 设置有安装在支撑打印机的托架组件的框架上的滑动调节构件的机构。 托架组件枢转地安装在由框架承载的支撑轴上。 滑动调节构件具有与多个锯齿形棘爪啮合的分度齿。 锯齿状棘爪纵向地设置在相对于水平面处于3至4度之间，优选为约3.44度的范围内的微小角度的表面上。 滑动构件在该成角度的表面上的水平移动导致托架组件围绕支撑轴枢转。 在这样的枢转中，承载在托架组件上的打印头被迫相对于压板移动，从而改变其间的纸张厚度间隙。 滑动构件经过止动齿的每个分度运动在间隙中提供0.003英寸的变化。 这种变化相当于单层和双层纸之间的差异。 因此，本发明提供了一种简单，低成本的滑动构件和锯齿形折痕表面，用于改变打印头和压板之间的纸间隙。
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公开(公告)号：US4971463A
公开(公告)日：1990-11-20
申请号：US495001
申请日：1990-03-16
申请人： Phillip B. Daley , James W. Lee
发明人： Phillip B. Daley , James W. Lee
CPC分类号： B41J35/28
摘要： A printer ribbon cassette mounting apparatus includes a pair of aligning posts and a pair of resilient latch members mounted on the lower surface of the cassette housing. The posts and latch members engage complementary openings in wall portions of the printer with which the cassette engages. Two restrainers extend from the lower surface of the cassette in parallel spaced relation to the latch members, and prevent excessive bending of said latch members.
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