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公开(公告)号:US20110201526A1
公开(公告)日:2011-08-18
申请号:US13033240
申请日:2011-02-23
申请人: Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Johathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人: Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Johathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号: C40B50/06
CPC分类号: C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译: 公开了用于核酸扩增的方法,其中将核酸模板,珠粒和扩增反应溶液乳化,并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。