-
公开(公告)号:US20060292611A1
公开(公告)日:2006-12-28
申请号:US11448462
申请日:2006-06-06
申请人: Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
发明人: Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
CPC分类号: C12Q1/6806 , C12N15/10 , C12N15/1093 , C12Q1/6809 , C12Q1/6869 , C12Q2537/155 , C12Q2525/191 , C12Q2521/301 , C12Q2533/107 , C12Q2531/125 , C12Q2521/319 , C12Q2521/125 , C12Q2565/518 , C12Q2563/131
摘要: The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.