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公开(公告)号:US20060292611A1
公开(公告)日:2006-12-28
申请号:US11448462
申请日:2006-06-06
申请人: Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
发明人: Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
CPC分类号: C12Q1/6806 , C12N15/10 , C12N15/1093 , C12Q1/6809 , C12Q1/6869 , C12Q2537/155 , C12Q2525/191 , C12Q2521/301 , C12Q2533/107 , C12Q2531/125 , C12Q2521/319 , C12Q2521/125 , C12Q2565/518 , C12Q2563/131
摘要: The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.
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公开(公告)号:US20050079510A1
公开(公告)日:2005-04-14
申请号:US10767899
申请日:2004-01-28
申请人: Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
发明人: Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
CPC分类号: C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译: 公开了用于核酸扩增的方法,其中将核酸模板,珠粒和扩增反应溶液乳化,并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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3.
公开(公告)号:US20090208965A1
公开(公告)日:2009-08-20
申请号:US12400975
申请日:2009-03-10
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6841
摘要: Automated methods for detecting cancer and related hyperplasias in biological samples.
摘要翻译: 用于检测生物样品中癌症和相关增生的自动化方法。
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公开(公告)号:US20160060689A1
公开(公告)日:2016-03-03
申请号:US14931833
申请日:2015-11-03
CPC分类号: C12Q1/6841 , G01N21/6428 , G01N21/6458 , G01N33/57496 , G01N2021/6421 , G01N2021/6439 , G02B21/0004 , G02B21/16
摘要: Automated methods for detecting cancer and related hyperplasias in biological samples.
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5.
公开(公告)号:US20080213769A1
公开(公告)日:2008-09-04
申请号:US11925123
申请日:2007-10-26
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6841 , G01N21/6428 , G01N21/6458 , G01N33/57496 , G01N2021/6421 , G01N2021/6439 , G02B21/0004 , G02B21/16
摘要: Automated methods for detecting cancer and related hyperplasias in biological samples.
摘要翻译: 用于检测生物样品中癌症和相关增生的自动化方法。
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6.
公开(公告)号:US20080182253A1
公开(公告)日:2008-07-31
申请号:US11924293
申请日:2007-10-25
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6841 , G01N21/6428 , G01N21/6458 , G01N33/57496 , G01N2021/6421 , G01N2021/6439 , G02B21/0004 , G02B21/16
摘要: Automated methods for detecting cancer and related hyperplasias in biological samples.
摘要翻译: 用于检测生物样品中癌症和相关增生的自动化方法。
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