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公开(公告)号：US20060292611A1
公开(公告)日：2006-12-28
申请号：US11448462
申请日：2006-06-06
申请人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
发明人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian Godwin , Stephen Hutchison , John Leamon , Gary Sarkis , Jan Simons
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1093 , C12Q1/6809 , C12Q1/6869 , C12Q2537/155 , C12Q2525/191 , C12Q2521/301 , C12Q2533/107 , C12Q2531/125 , C12Q2521/319 , C12Q2521/125 , C12Q2565/518 , C12Q2563/131
摘要： The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.
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公开(公告)号：US07601499B2
公开(公告)日：2009-10-13
申请号：US11448462
申请日：2006-06-06
申请人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian C. Godwin , Stephen Kyle Hutchison , John Harris Leamon , Gary James Sarkis , Jan Fredrik Simons
发明人： Jan Berka , Zhoutao Chen , Michael Egholm , Brian C. Godwin , Stephen Kyle Hutchison , John Harris Leamon , Gary James Sarkis , Jan Fredrik Simons
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1093 , C12Q1/6809 , C12Q1/6869 , C12Q2537/155 , C12Q2525/191 , C12Q2521/301 , C12Q2533/107 , C12Q2531/125 , C12Q2521/319 , C12Q2521/125 , C12Q2565/518 , C12Q2563/131
摘要： The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.
摘要翻译： 本发明提供了制备靶核酸片段以产生包含靶核酸两端的较小核酸的方法。 具体地，本发明提供克隆和DNA操作策略，以将大靶核酸的两端分离成单个小型DNA构建体用于快速克隆，测序或扩增。
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公开(公告)号：US20050079510A1
公开(公告)日：2005-04-14
申请号：US10767899
申请日：2004-01-28
申请人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
发明人： Jan Berka , Yi-Ju Chen , John Leamon , Steve Lefkowitz , Kenton Lohman , Vinod Makhijani , Jonathan Rothberg , Gary Sarkis , Maithreyan Srinivasan , Michael Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US20080132693A1
公开(公告)日：2008-06-05
申请号：US11982095
申请日：2007-10-31
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US07264929B2
公开(公告)日：2007-09-04
申请号：US10299180
申请日：2002-11-19
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。
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公开(公告)号：US06554985B2
公开(公告)日：2003-04-29
申请号：US09374174
申请日：1999-08-13
IPC分类号： B01D5702
CPC分类号： G01N27/44747
摘要： This invention relates to methods and formulations for the separation of biological macromolecules according to their size using capillary electrophoresis with improved polyacrylamide matrixes under denaturing conditions.
摘要翻译： 本发明涉及用于在变性条件下使用毛细管电泳和改进的聚丙烯酰胺基质分离生物大分子的方法和制剂。
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公开(公告)号：US20130078638A1
公开(公告)日：2013-03-28
申请号：US13618334
申请日：2012-09-14
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化并扩增核酸模板以提供附着于头部的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US08012690B2
公开(公告)日：2011-09-06
申请号：US11982095
申请日：2007-10-31
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译： 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。
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公开(公告)号：US07244559B2
公开(公告)日：2007-07-17
申请号：US09814338
申请日：2001-03-21
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
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公开(公告)号：US07211390B2
公开(公告)日：2007-05-01
申请号：US10788529
申请日：2004-02-26
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo
CPC分类号： C12Q1/6837 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B82Y30/00 , C12Q1/6827 , C12Q1/6869 , C12Q1/6874 , G01N21/6452 , G01N2201/08 , G01N2201/0846 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.
摘要翻译： 本文公开了用于测序核酸的方法和装置。 一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序 通过检测序列副产物如焦磷酸盐的存在来扩增分子。
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