公开(公告)号：US12215354B2

公开(公告)日：2025-02-04

申请号：US17749735

申请日：2022-05-20

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells.

公开(公告)号：US20240417692A1

公开(公告)日：2024-12-19

申请号：US18627889

申请日：2024-04-05

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071 ,  C12N5/0735

Abstract: The present invention provides methods to promote differentiation of pluripotent stem cells to pancreatic endoderm cells expressing PDX1, NKX6.1, and HB9. In particular, the methods encompass culturing Stage 4 to Stage 6 cells with a thyroid hormone (e.g. T3), an ALK5 inhibitor, or both.

公开(公告)号：US20230287353A1

公开(公告)日：2023-09-14

申请号：US17968675

申请日：2022-10-18

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

CPC classification number: C12N5/0678 ,  C12N5/0676 ,  C12N2501/105 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/70 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2501/15 ,  C12N2501/40 ,  C12N2501/727

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells.

公开(公告)号：US20210079353A1

公开(公告)日：2021-03-18

申请号：US16950244

申请日：2020-11-17

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

Abstract: The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.

公开(公告)号：US20200208116A1

公开(公告)日：2020-07-02

申请号：US16817413

申请日：2020-03-12

Applicant: Janssen Biotech, Inc.

Inventor： Sebastian Rieck ,  Alireza Rezania

IPC: C12N5/071 ,  C12Q1/6806 ,  C07K14/72 ,  A61K35/39

Abstract: The invention provides for methods of differentiating pancreatic endocrine cells into pancreatic beta cells expressing PDX1, NKX6.1, MAFA, UCN3 and SLC2A. These pancreatic beta cells may be obtained by step-wise differentiation of pluripotent stem cells. The pancreatic beta cells exhibit glucose-dependent mitochondrial respiration and glucose-stimulated insulin secretion similar to islet cells.

公开(公告)号：US10421948B2

公开(公告)日：2019-09-24

申请号：US15683594

申请日：2017-08-22

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071

Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

公开(公告)号：US20170362572A1

公开(公告)日：2017-12-21

申请号：US15622931

申请日：2017-06-14

Applicant: Janssen Biotech, Inc.

Inventor： Sebastian Rieck ,  Alireza Rezania

IPC: C12N5/071 ,  C12Q1/68 ,  A61K35/39 ,  C07K14/72

CPC classification number: C12N5/0678 ,  A61K35/39 ,  C07K14/72 ,  C12N5/0677 ,  C12N5/068 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/335 ,  C12N2501/345 ,  C12N2501/375 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/90 ,  C12Q1/6806

Abstract: The invention provides for methods of differentiating pancreatic endocrine cells into pancreatic beta cells expressing PDX1, NKX6.1, MAFA, UCN3 and SLC2A. These pancreatic beta cells may be obtained by step-wise differentiation of pluripotent stem cells. The pancreatic beta cells exhibit glucose-dependent mitochondrial respiration and glucose-stimulated insulin secretion similar to islet cells.

公开(公告)号：US20170183626A1

公开(公告)日：2017-06-29

申请号：US15457952

申请日：2017-03-13

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/15

Abstract: The present invention provides methods to promote the proliferation of undifferentiated pluripotent stem cells in defined media. Specifically, the invention provides a defined cell culture formulation for the culture, maintenance, and expansion of pluripotent stem cells, wherein culturing stem cells in the defined cell culture formulation maintains the pluripotency and karyotypic stability of the cells for at least 10 passages. Further disclosed is a cell population, grown under defined media conditions, that expresses OCT4, SOX2, NANOG, and FOXA2.

公开(公告)号：US20170081634A1

公开(公告)日：2017-03-23

申请号：US15368359

申请日：2016-12-02

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

CPC classification number: C12N5/0608 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage.

公开(公告)号：US20130189777A1

公开(公告)日：2013-07-25

申请号：US13708369

申请日：2012-12-07

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells.