公开(公告)号：US20060248117A1

公开(公告)日：2006-11-02

申请号：US11106396

申请日：2005-04-14

申请人： Jason Bell ,  Mark Cavage ,  Kristin Hazlewood ,  Richard Heller

发明人： Jason Bell ,  Mark Cavage ,  Kristin Hazlewood ,  Richard Heller

IPC分类号： G06F17/00 ,  G06F7/00

CPC分类号： G06F17/30067 ,  Y10S707/99943 ,  Y10S707/99953

摘要： Each LDIF entry of a directory tree is read, split to a domain of LDIF fragments (corresponding to backend servers) and written to each LDIF fragment. The split may be accomplished through a hash function, establishing, for that iteration of LDIF entry, a write file. The LDIF entry is appended to the write file. A subsequent LDIF entry is read. A corresponding LDIF fragment is determined, which need not be different from the LDIF fragment to which the first LDIF entry was written. The current LDIF entry is written to the currently selected write file. The process continues until all LDIF entries are exhausted from the directory tree. LDIF fragments are each copied to distinct backend servers, where, each LDIF fragment may be loaded into a distributed directory data structure.

摘要翻译： 读取目录树的每个LDIF条目，分割为LDIF片段的域（对应于后端服务器）并写入每个LDIF片段。 可以通过散列函数完成拆分，为LDIF条目的迭代建立写入文件。 LDIF条目附加到写入文件。 读取随后的LDIF条目。 确定对应的LDIF片段，其不需要与写入第一LDIF条目的LDIF片段不同。 当前的LDIF条目写入当前选择的写入文件。 该过程继续，直到所有LDIF条目从目录树中用尽。 LDIF片段都被复制到不同的后端服务器，其中每个LDIF片段可以被加载到分布式目录数据结构中。

公开(公告)号：US20050076335A1

公开(公告)日：2005-04-07

申请号：US10677659

申请日：2003-10-02

申请人： Mark Cavage ,  Kristin Hazlewood ,  Richard Heller ,  Gary Williams

发明人： Mark Cavage ,  Kristin Hazlewood ,  Richard Heller ,  Gary Williams

IPC分类号： G06F9/46

CPC分类号： G06F9/3851 ,  G06F11/0715 ,  G06F11/0793 ,  H04L63/1458

摘要： A method is presented for processing data in a multithreaded application to alleviate impaired or substandard performance conditions. Work items that are pending processing by the multithreaded application are placed into a data structure. The work items are processed by a plurality of threads within the multithreaded application in accordance with a first algorithm, e.g., first-in first-out (FIFO). A thread within the multithreaded application is configured apart from the plurality of threads such that it processes work items in accordance with a second algorithm that differs from the first algorithm, thereby avoiding the impairing condition. For example, the thread may process a pending work item only if it has a particular characteristic. The thread restricts its own processing of work items by intermittently evaluating workflow conditions for the plurality of threads; if the workflow conditions improve or are unimpaired, then the thread does not process any work items.

摘要翻译： 提出了一种在多线程应用程序中处理数据以减轻受损或不合格的性能条件的方法。 多线程应用程序待处理的工作项被放置在数据结构中。 根据第一算法，例如先进先出（FIFO），由多线程应用程序内的多个线程处理工作项。 多线程应用程序内的线程被配置为与多个线程分开，使得它根据与第一算法不同的第二算法处理工作项，从而避免损害状况。 例如，线程可以仅在具有特定特征的情况下处理待处理的工作项。 线程通过间歇地评估多个线程的工作流条件来限制其自己的工作项的处理; 如果工作流条件改善或未受损，则线程不处理任何工作项。

公开(公告)号：US09610364B1

公开(公告)日：2017-04-04

申请号：US14589463

申请日：2015-01-05

申请人： Richard Heller ,  Melinda Lee Lucas ,  Loree C. Heller

发明人： Richard Heller ,  Melinda Lee Lucas ,  Loree C. Heller

IPC分类号： A61K48/00 ,  C12N15/87 ,  A61N1/32

CPC分类号： A61K48/0083 ,  A61K38/00 ,  A61K48/005 ,  A61N1/327 ,  C07K14/70532

摘要： A method of treating cancerous tumors is presented herein. The method includes injecting an effective dose of a plasmid encoded for IL-12, B7-1 or IL-15 into a cancerous tumor and subsequently administering at least one high voltage, short duration pulse to the tumor. The electroporation pulses may be administered at at least 700V/cm for a duration of less than 1 millisecond. The intratumor treatments with electroporation may be administered in at least a two-treatment protocol with the time between treatments being about 7 days. The intratumor treatments with electroporation may be administered in a three-treatment protocol with a time of four days between the first and second treatments and a time of three days between the second and third treatments. It was found that the intratumor treatments using electroporation not only resulted in tumor regression but also induced an immune memory response which prevented the formation of new tumors.

公开(公告)号：US07769440B2

公开(公告)日：2010-08-03

申请号：US10605126

申请日：2003-09-10

申请人： Andrew M. Hoff ,  Richard Gilbert ,  Richard Heller ,  Mark J. Jaroszeski

发明人： Andrew M. Hoff ,  Richard Gilbert ,  Richard Heller ,  Mark J. Jaroszeski

IPC分类号： A61N1/30

CPC分类号： A61N1/30 ,  A61N1/0424 ,  A61N1/325 ,  C12N15/87

摘要： The present invention is a device for electromanipulation of chemical species in vivo relative to a target tissue including a nonconductive, conformable array base adapted to be placed coincident to the target tissue, a plurality of electrode elements projecting from the array base towards the target tissue, the electrode elements addressable individually, an electrical source coupled to the plurality of electrodes, a control means interposed between the electrical source and the plurality of electrode elements and in circuit communication therein, the control means adapted to establish an electrical potential between at least two electrodes, and a delivery means adapted to introduce chemical species to the target tissue.

摘要翻译： 本发明是一种用于相对于靶组织在体内电化学物质的装置，该靶组织包括适于与靶组织重合放置的非导电适形阵列基底，从阵列基底朝向靶组织突出的多个电极元件， 所述电极元件单独寻址，耦合到所述多个电极的电源，插入在所述电源和所述多个电极元件之间并且与所述多个电极元件中的电路连通的控制装置，所述控制装置适于在至少两个电极之间建立电位 以及适于将化学物种引入目标组织的递送装置。

公开(公告)号：US20100109757A1

公开(公告)日：2010-05-06

申请号：US12577985

申请日：2009-10-13

申请人： Maciej Wiatr ,  Richard Heller ,  Rolf Geilenkeuser

发明人： Maciej Wiatr ,  Richard Heller ,  Rolf Geilenkeuser

IPC分类号： G05F1/10

CPC分类号： G06F1/26

摘要： By maintaining a substantially constant total die power during the entire lifetime of sophisticated integrated circuits, the performance degradation may be reduced. Consequently, greatly reduced guard bands for parts classification may be used compared to conventional strategies in which significant performance degradation may occur when the integrated circuits are operated on the basis of a constant supply voltage.

摘要翻译： 通过在复杂集成电路的整个寿命期间保持基本上恒定的总管芯功率，可以降低性能下降。 因此，与常规策略相比，可以大大减少用于部件分类的保护带，其中当基于恒定的电源电压来操作集成电路时可能发生显着的性能劣化。

公开(公告)号：US20090131994A1

公开(公告)日：2009-05-21

申请号：US12246958

申请日：2008-10-07

申请人： Jose I. Rey ,  Richard Gilbert ,  Mark J. Jaroszeski ,  Richard Heller

发明人： Jose I. Rey ,  Richard Gilbert ,  Mark J. Jaroszeski ,  Richard Heller

IPC分类号： A61N1/05 ,  C12N13/00

CPC分类号： A61N1/327 ,  A61N1/325 ,  C12M35/02

摘要： The present invention provides a system and method of directing, focusing, or concentrating electrical charges within a defined electric field so that these charges can be used to exert forces on cells and tissues in vivo and/or cell cultures in vitro. The present invention reduces and/or eliminates the damage at a target site that would normally be caused by an electrode that acts as a current source or sink to accomplish the same task.

摘要翻译： 本发明提供了在限定的电场内引导，聚焦或集中电荷的系统和方法，使得这些电荷可以用于在体外对体内和/或细胞培养物上的细胞和组织施加力。 本发明减少和/或消除了通常由用作电流源或吸收器来完成相同任务的电极引起的目标位置处的损伤。

公开(公告)号：US06937890B2

公开(公告)日：2005-08-30

申请号：US09772561

申请日：2001-01-30

申请人： Mark J. Jaroszeski ,  Richard Gilbert ,  Richard Heller

发明人： Mark J. Jaroszeski ,  Richard Gilbert ,  Richard Heller

IPC分类号： A61N1/30 ,  A61N1/32 ,  C12M1/42

CPC分类号： A61N1/327 ,  A61N1/0424 ,  A61N1/0476

摘要： The manipulator includes a support and at least one member affixed to and extending away from the support. The member has at least two differentially activatable areas of conductivity. The members are configured to establish a first electromagnetic field in vivo between selected areas of conductivity sufficient to manipulate a molecule relative to a target tissue and to establish a second, typically higher, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. Restraining means are also described for restricting movement of the members with relation to each other. One method of using the device is for enhancing the delivery of a molecule into a tissue site; another is for poration of the tissue alone or in combination with the migration. The target tissue may include a tumor, organ, or wound site.

摘要翻译： 操纵器包括支撑件和固定到支撑件上并远离支撑件的至少一个构件。 该构件具有至少两个差异可激活的导电区域。 构件被构造成在体内在选择的导电区域之间建立第一电磁场，以便相对于目标组织操纵分子并建立足以引起靶内细胞膜瞬时通透的第二，通常较高的电磁场 组织。 还描述了用于限制构件相对于彼此的运动的限制装置。 使用该装置的一种方法是用于增强分子进入组织部位的递送; 另一种是单独或与移植相结合的组织的培养。 靶组织可以包括肿瘤，器官或伤口部位。

公开(公告)号：US06929949B1

公开(公告)日：2005-08-16

申请号：US10250219

申请日：2003-06-13

申请人： Drew Hoff ,  Mark J. Jaroszeski ,  Richard Gilbert ,  Richard Heller

发明人： Drew Hoff ,  Mark J. Jaroszeski ,  Richard Gilbert ,  Richard Heller

IPC分类号： C12N13/00 ,  C12N15/87

CPC分类号： C12N15/87 ,  C12M35/08 ,  C12N13/00

摘要： The present invention provides a method and apparatus adapted to facilitate the entry of a preselected molecule into the intracellular space of a cellular sample through the use of ions generated by a corona charge source. With the present method and apparatus, molecules are manipulated within cells and in the extracellular space surrounding the cells. Manipulation enhances the permeability of cell barriers to allow the subsequent introduction of molecules of interest into the interior of a cell.

摘要翻译： 本发明提供了一种适于通过使用由电晕电荷源产生的离子来促进预选分子进入细胞样品的细胞内空间的方法和装置。 利用本发明的方法和装置，分子在细胞内和细胞周围的细胞外空间中被操纵。 操纵增强细胞屏障的渗透性，以允许随后将感兴趣的分子引入细胞内部。

公开(公告)号：US5827736A

公开(公告)日：1998-10-27

申请号：US782509

申请日：1997-01-09

申请人： Richard Heller ,  Don F. Cameron ,  Paul R. Sanberg ,  Mark J. Jaroszeski

发明人： Richard Heller ,  Don F. Cameron ,  Paul R. Sanberg ,  Mark J. Jaroszeski

IPC分类号： A61K35/12 ,  A61K35/39 ,  A61K35/48 ,  A61K38/00 ,  C12N5/071 ,  C12N5/16 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0683 ,  A61K35/39 ,  A61K35/48 ,  C12N5/16 ,  A61K2035/122 ,  A61K35/12 ,  A61K38/00

摘要： A purified and isolated Sertoli cell and secretory cell hybrid, or an aggregate of these two cells, wherein the secretory cells preferably are pancreatic islet cells and chromaffin cells characterized by being a) capable of survival in situ after transplantation; b) able to provide immunoprotection for the hybrid cells when transplanted; and c) able to provide a mechanism for prolonged viability and cellular functionality of the transplanted hybrid cells wherein the hybrid maintains both the immunoprotection characteristics of the Sertoli cell and the secretory function of the secretory cell.

摘要翻译： 纯化和分离的Sertoli细胞和分泌细胞杂交体或这两种细胞的聚集体，其中分泌细胞优选是胰岛细胞和嗜铬细胞，其特征在于a）能够在移植后就地存活; b）能够在移植时为杂交细胞提供免疫保护; 和c）能够提供移植杂交细胞的延长活力和细胞功能的机制，其中所述杂合物维持所述Sertoli细胞的免疫保护特征和分泌细胞的分泌功能。

公开(公告)号：US5702359A

公开(公告)日：1997-12-30

申请号：US467566

申请日：1995-06-06

申请人： Gunter A. Hofmann ,  Richard A. Gilbert ,  Yasuhiko Hayakawa ,  Richard Heller ,  Mark J. Jaroszeski

发明人： Gunter A. Hofmann ,  Richard A. Gilbert ,  Yasuhiko Hayakawa ,  Richard Heller ,  Mark J. Jaroszeski

IPC分类号： A61N1/30 ,  A61M37/00 ,  A61N1/32

CPC分类号： A61N1/327 ,  A61N1/325 ,  C12M35/02 ,  A61N1/0502

摘要： An electrode apparatus for the application of electroporation to a portion of the body of a patient, comprises a support member, a pair of electrodes adjustably mounted on the support member for movement toward and away from one another for positioning on opposite sides of a body portion to be electroporated, a sensing element for sensing a distance between the electrodes and generating a distance signal proportionate to the distance between said electrodes, and means responsive to said distance signal for applying pulses of high amplitude electric signal to the electrodes proportionate to the distance between said electrodes.

摘要翻译： 用于将电穿孔施加到患者身体的一部分的电极装置包括支撑构件，可调节地安装在支撑构件上的一对电极，用于朝向和远离彼此移动以在身体部分的相对侧上定位 用于感测电极之间的距离并且产生与所述电极之间的距离成比例的距离信号的感测元件，以及响应于所述距离信号的装置，用于将高振幅电信号的脉冲施加到电极的比例 所述电极。