公开(公告)号：US09657272B2

公开(公告)日：2017-05-23

申请号：US14498089

申请日：2014-09-26

Applicant: Georgetown University

Inventor： Richard Schlegel ,  Xuefeng Liu

IPC: C12N5/071 ,  C12Q1/68 ,  G01N33/50

CPC classification number: C12N5/0683 ,  C12N5/0625 ,  C12N5/0631 ,  C12N5/067 ,  C12N5/0688 ,  C12N2500/05 ,  C12N2500/40 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2503/02 ,  C12N2510/04 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/5091

Abstract: The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.

公开(公告)号：US20170114327A1

公开(公告)日：2017-04-27

申请号：US15277919

申请日：2016-09-27

Applicant: CELLARITY TECHNOLOGIES, INC.

Inventor： Constance M. JOHN ,  Alpa MAHUVAKAR

IPC: C12N5/071 ,  A61K35/48

CPC classification number: C12N5/0683 ,  A61K35/48 ,  C12N2501/31 ,  C12N2502/246 ,  C12N2503/00 ,  C12N2510/00

Abstract: Technology for the isolation and propagation of primary human Sertoli cells from normal testes tissue, including cultures of proliferative primary human Sertoli cells for research and clinical applications, and a pharmaceutical composition for cell therapy, ex vivo gene therapy, and for the reduction of autoimmune, allograft, and xenograft immune reactions.

公开(公告)号：US20170073635A1

公开(公告)日：2017-03-16

申请号：US15258751

申请日：2016-09-07

Applicant: PROPAGENIX INC.

Inventor： Chengkang ZHANG

IPC: C12N5/071 ,  C12N15/85

CPC classification number: C12N5/0602 ,  C12N5/0683 ,  C12N5/0688 ,  C12N15/85 ,  C12N2500/30 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/15 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2533/54

Abstract: The technology relates in part to methods and compositions for ex vivo proliferation and expansion of epithelial cells.

Abstract translation: 该技术部分地涉及上皮细胞离体增殖和扩增的方法和组合物。

公开(公告)号：US09434922B2

公开(公告)日：2016-09-06

申请号：US14639454

申请日：2015-03-05

Applicant: Washington State University

Inventor： Jon Michael Oatley ,  Melissa Joan Oatley

IPC: A61B17/43 ,  C12N5/00 ,  C12N5/076 ,  C12N5/073 ,  C12N5/071 ,  C12M1/00 ,  A61K35/52 ,  C12N5/077 ,  C12N15/873 ,  A01K67/027 ,  A61B17/425 ,  A61D19/00

CPC classification number: C12N5/061 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  A61B17/425 ,  A61D19/00 ,  A61K35/52 ,  C12M21/00 ,  C12N5/0602 ,  C12N5/0603 ,  C12N5/0656 ,  C12N5/0683 ,  C12N15/873 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/246 ,  C12N2510/04 ,  C12N2533/90

Abstract: The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

公开(公告)号：US20150329829A1

公开(公告)日：2015-11-19

申请号：US14722065

申请日：2015-05-26

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： Michael M. SHEN ,  Chee Wai CHUA ,  Ming LEI

IPC: C12N5/071 ,  G01N33/574 ,  C12N5/09 ,  G01N33/50

CPC classification number: C12N5/0683 ,  C12N5/0693 ,  C12N2501/11 ,  C12N2501/392 ,  G01N33/5011 ,  G01N33/57434

Abstract: The invention discloses a methodology for the culture of prostate tissue organoids from mouse and human prostate.

Abstract translation: 本发明公开了一种从小鼠和人前列腺培养前列腺组织有机体的方法。

公开(公告)号：US09161901B2

公开(公告)日：2015-10-20

申请号：US12918265

申请日：2009-02-23

Applicant: Arun Kumar ,  Shyam S. Mohapatra ,  Don F. Cameron

Inventor： Arun Kumar ,  Shyam S. Mohapatra ,  Don F. Cameron

IPC: A61K35/48 ,  A61K9/00 ,  A61B5/00 ,  A61K9/50 ,  A61K9/51 ,  A61K31/12 ,  A61K47/36 ,  A61K47/46 ,  A61K47/48 ,  A61K49/00 ,  B82Y5/00

CPC classification number: A61K31/12 ,  A61B5/0071 ,  A61B5/4848 ,  A61K9/0019 ,  A61K9/5068 ,  A61K9/5161 ,  A61K47/36 ,  A61K47/46 ,  A61K47/61 ,  A61K47/6901 ,  A61K47/6939 ,  A61K49/0043 ,  A61K49/0093 ,  A61K49/0097 ,  B82Y5/00 ,  C12N5/0683

Abstract: A method of delivering a compound of interest to the lungs of a subject by the intravenous injection of Sertoli cells loaded with a plurality of chitosan nanoparticles coupled with the compound of interest is provided. Testis-derived rat Sertoli cells were pre-loaded with chitosan nanoparticles coupled with or without the drug curcumin, pre-labeled with a fluorescent cell marker and then injected intravenously into the control or asthmatic mouse model host. Intact pre-loaded, pre-labeled Sertoli cells were present in the lungs at 15 minutes post-injection, appeared entrapped in the pulmonary pre-capillary vascular bed around alveolar sacs but were not present one hour post-injection although Sertoli cell label and cellular debris was. Most of the injected nanoparticle load (70%) and curcumin load (80%) was present in the lungs 15 minutes post-injection, and remained at 70% and 80%, respectively, one hour post-injection.

Abstract translation: 提供了通过静脉内注射负载有与感兴趣的化合物结合的多个壳聚糖纳米颗粒的Sertoli细胞将感兴趣的化合物递送到受试者的肺的方法。 睾丸衍生的大鼠支持细胞预先装载有或不含药物姜黄素的壳聚糖纳米颗粒，用荧光细胞标记物预先标记，然后静脉内注射到对照或哮喘小鼠模型宿主中。 完整的预加载，预先标记的Sertoli细胞在注射后15分钟存在于肺中，似乎被夹带在肺泡囊周围的肺前毛细血管血管床中，但注射后1小时不存在，尽管Sertoli细胞标记和细胞 碎片是。 在注射后15分钟，大部分注射的纳米粒子负载（70％）和姜黄色素负荷（80％）分别存在于肺中，并且在注射后1小时分别保持在70％和80％。

公开(公告)号：US20150175957A1

公开(公告)日：2015-06-25

申请号：US14639454

申请日：2015-03-05

Applicant: Washington State University

Inventor： Jon Michael Oatley ,  Melissa Joan Oatley

IPC: C12N5/076 ,  A61D19/00 ,  A61B17/425

CPC classification number: C12N5/061 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  A61B17/425 ,  A61D19/00 ,  A61K35/52 ,  C12M21/00 ,  C12N5/0602 ,  C12N5/0603 ,  C12N5/0656 ,  C12N5/0683 ,  C12N15/873 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/246 ,  C12N2510/04 ,  C12N2533/90

Abstract: The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

Abstract translation: 本发明涉及可以长期保持并且不含饲料的未分化精原干细胞的生产和培养。 所得的无饲料种群可以用于许多方案中的任一种，包括产生后代公牛。 本发明包括成功富集牛精原干细胞，新型细胞系和用于其的其它组分所需的新方法，以及所得的干细胞组合物。

公开(公告)号：US20130309681A1

公开(公告)日：2013-11-21

申请号：US13885078

申请日：2011-11-11

Applicant: Richard Schlegel ,  Xuefeng Liu

Inventor： Richard Schlegel ,  Xuefeng Liu

IPC: C12N5/071

CPC classification number: C12N5/0683 ,  C12N5/0625 ,  C12N5/0631 ,  C12N5/067 ,  C12N5/0688 ,  C12N2500/05 ,  C12N2500/40 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2503/02 ,  C12N2510/04 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/5091

Abstract: The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.

公开(公告)号：US07846726B2

公开(公告)日：2010-12-07

申请号：US10366032

申请日：2003-02-12

Applicant: Ronghao Li ,  Zhuangyu Pan

Inventor： Ronghao Li ,  Zhuangyu Pan

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC classification number: C12N5/0685 ,  A61K39/00 ,  A61K2039/515 ,  C12N5/0683 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/117 ,  C12N2501/195 ,  C12N2501/392 ,  C12N2501/70 ,  C12N2502/246 ,  C12N2503/02 ,  C12N2506/25

Abstract: This invention discloses a substantially pure population of human urinary bladder-derived epithelial cells and methods of isolating and culturing the urinary bladder-derived epithelial cells. In addition, several uses of human urinary bladder-derived epithelial cells and cells differentiating therefrom are disclosed herein.

Abstract translation: 本发明公开了一种基本纯净的人膀胱衍生上皮细胞群，以及分离和培养膀胱来源的上皮细胞的方法。 此外，本文公开了人膀胱衍生的上皮细胞和与其分化的细胞的几种用途。

公开(公告)号：US06982168B1

公开(公告)日：2006-01-03

申请号：US08913770

申请日：1997-01-30

Applicant: Suzanne L. Topalian ,  W. Marston Linehan ,  Robert K. Bright ,  Cathy D. Vocke

Inventor： Suzanne L. Topalian ,  W. Marston Linehan ,  Robert K. Bright ,  Cathy D. Vocke

IPC: C12N15/85

CPC classification number: A61K38/00 ,  A61K39/00 ,  C07K16/18 ,  C12N5/0683 ,  C12N5/0693 ,  C12N15/85

Abstract: The present invention relates to immortalized, malignant, human, adult prostate epithelial cell lines or cell lines derived therefrom useful in the diagnosis and treatment of prostate cancer. More particularly, the present invention relates to cloned, immortalized, malignant, human, adult prostate epithelial cell lines and uses of these cell lines for the diagnosis and treatment of cancer. Furthermore, the present invention provides for the characterization of said cell lines through the analysis of specific chromosomal deletions.

Abstract translation: 本发明涉及可用于诊断和治疗前列腺癌的永生化，恶性，人类，成年前列腺上皮细胞系或其衍生的细胞系。 更具体地，本发明涉及克隆的，永生化的，恶性的，人类，成年前列腺上皮细胞系和这些细胞系用于诊断和治疗癌症的用途。 此外，本发明通过分析特异性染色体缺失来提供所述细胞系的表征。