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    Method and support for biological analysis using oligonucleotides comprising a marker capable of enzymatic activation
    1.
    发明申请
    Method and support for biological analysis using oligonucleotides comprising a marker capable of enzymatic activation 失效
    使用包含能够进行酶活化的标记的寡核苷酸进行生物分析的方法和支持

    公开(公告)号：US20050042610A1

    公开(公告)日：2005-02-24

    申请号：US10484178

    申请日：2002-07-12

    申请人： Jean-Luc Decout ,  Marc Fontecave ,  Cecile Dueymes

    发明人： Jean-Luc Decout ,  Marc Fontecave ,  Cecile Dueymes

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/566 ,  G01N33/58

    CPC分类号： C12Q1/6816 ,  C12Q2563/125

    摘要： The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps: a) contacting the targets to be analyzed (ICAM-20) with oligonucleotide probes (ISIS) labeled with a cofactor A of an enzyme E; b) adding, to the reaction medium, enzyme E corresponding to cofactor A and a substrate S of enzyme E, substrate S being converted by enzyme E into a compound C; c) measuring a signal representative of the activity of enzyme E on substrate S, for example the fluorescence intensity (I) of substrate S; and d) comparing this signal with the signal obtained when the oligonucleotide probes labeled with cofactor A (ISIS) are contacted with enzyme E and substrate S, under the same conditions, but in the absence of the targets, the difference between the two signals indicating the presence of complementary targets (ICAM-20) of the oligonucleotide probes.

    摘要翻译： 本发明涉及一种用于分析DNA或RNA型生物靶标的方法，其包括以下步骤：a）使待分析的靶标（ICAM-20）与用酶E的辅因子A标记的寡核苷酸探针（ISIS）接触; b）向反应介质中加入对应于辅因子A的酶E和酶E底物S，底物S由酶E转化成化合物C; c）测量表示酶E在底物S上的活性的信号，例如底物S的荧光强度（I）; 和d）将该信号与在辅酶A（ISIS）标记的寡核苷酸探针与酶E和底物S接触时所获得的信号进行比较，但在不存在目标的情况下，两个信号之间的差异表明 存在寡核苷酸探针的互补靶（ICAM-20）。

    Method and support for biological analysis using oligonucleotides comprising a marker capable of enzymatic activation
    3.
    发明授权
    Method and support for biological analysis using oligonucleotides comprising a marker capable of enzymatic activation 失效
    使用包含能够进行酶活化的标记的寡核苷酸进行生物分析的方法和支持

    公开(公告)号：US07220543B2

    公开(公告)日：2007-05-22

    申请号：US10484178

    申请日：2002-07-12

    申请人： Jean-Luc Decout ,  Marc Fontecave ,  Cécile Dueymes

    发明人： Jean-Luc Decout ,  Marc Fontecave ,  Cécile Dueymes

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q2563/125

    摘要： The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps: a) contacting the targets to be analyzed (ICAM-20) with oligonucleotide probes (ISIS) labeled with a cofactor A of an enzyme E; b) adding, to the reaction medium, enzyme E corresponding to cofactor A and a substrate S of enzyme E, substrate S being converted by enzyme E into a compound C; c) measuring a signal representative of the activity of enzyme E on substrate S, for example the fluorescence intensity (I) of substrate S; and d) comparing this signal with the signal obtained when the oligonucleotide probes labeled with cofactor A (ISIS) are contacted with enzyme E and substrate S, under the same conditions, but in the absence of the targets, the difference between the two signals indicating the presence of complementary targets (ICAM-20) of the oligonucleotide probes.

    摘要翻译： 本发明涉及一种用于分析DNA或RNA型生物靶标的方法，其包括以下步骤：a）使待分析的靶标（ICAM-20）与用酶E的辅因子A标记的寡核苷酸探针（ISIS）接触; b）向反应介质中加入对应于辅因子A的酶E和酶E底物S，底物S由酶E转化成化合物C; c）测量表示酶E在底物S上的活性的信号，例如底物S的荧光强度（I）; 和d）将该信号与在辅酶A（ISIS）标记的寡核苷酸探针与酶E和底物S接触时所获得的信号进行比较，但在不存在目标的情况下，两个信号之间的差异表明 存在寡核苷酸探针的互补靶（ICAM-20）。

    BIODEGRADABLE ELECTROCONDUCTING NANOWIRE, METHOD OF MANUFACTURE AND USES THEREOF
    7.
    发明申请
    BIODEGRADABLE ELECTROCONDUCTING NANOWIRE, METHOD OF MANUFACTURE AND USES THEREOF 有权
    可生物降解的电极纳米管，其制造方法及其用途

    公开(公告)号：US20140148575A1

    公开(公告)日：2014-05-29

    申请号：US14003560

    申请日：2012-03-06

    申请人： Vincent Forge ,  Christophe Horvath ,  Marc Fontecave ,  Nicolas Duraffourg

    发明人： Vincent Forge ,  Christophe Horvath ,  Marc Fontecave ,  Nicolas Duraffourg

    IPC分类号： C07K14/00

    CPC分类号： C07K14/001 ,  B82Y30/00 ,  B82Y40/00 ,  C07K14/4711 ,  C07K2319/735

    摘要： The subject matter of the invention is an electroconducting nanowire, comprising an amyloid fibre which results from the self-assembling of peptides comprising a prion domain or a derivative of this domain and which is functionalized by electron transporting peptides, each comprising amino acids bound to one or more atoms of iron.Its subject matter is also a method of manufacturing this nanowire as well as the uses of said nanowire.Uses: manufacture of sensors for the detection of chemical or biological species, of nano-batteries, of radio-identification systems, of molecular memory systems, etc.

    摘要翻译： 本发明的主题是导电纳米线，其包含淀粉样蛋白纤维，其由包含朊病毒结构域或该结构域的衍生物的肽的自组装产生，并且其由电子传输肽官能化，每个包含与一个结合的氨基酸 或更多的铁原子。 其主题也是制造该纳米线的方法以及所述纳米线的用途。 用途：制造用于检测化学或生物物种，纳米电池，无线电识别系统，分子存储系统等的传感器。