利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

26 条记录 (耗时 0.033 秒)

    Sir2 products and activities
    4.
    发明授权
    Sir2 products and activities 失效
    Sir2产品和活动

    公开(公告)号：US07741295B2

    公开(公告)日：2010-06-22

    申请号：US12231880

    申请日：2008-09-05

    申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

    发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

    IPC分类号： A61K31/70 ,  A01N43/04

    CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

    摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

    摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

    In vitro transposition of article transposons
    5.
    发明授权
    In vitro transposition of article transposons 失效
    文章转座子的体外转座

    公开(公告)号：US5968785A

    公开(公告)日：1999-10-19

    申请号：US796364

    申请日：1997-02-06

    申请人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

    发明人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

    IPC分类号： C12N15/09 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/64 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12N15/90 ,  C12Q1/6869 ,  C12Q1/6895

    摘要： We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

    摘要翻译： 我们开发了有效的创建人工转座子的方法，并将这些转座子在体外插入到质粒靶标中，主要用于测序和测序DNA。 已经将工程改造成质粒，将几乎任何DNA序列或序列的组合转化成人工转座子; 因此，可以容易地设计和构造含有任何期望特征的定制转座子。 然后使用存在于酵母Ty1病毒样颗粒中的整合酶活性，将该转座子在体外有效地插入质粒靶标中。 与转座子末端互补的引物可用于对任何转座子插入侧翼的DNA进行序列比对。

    Sir2 products and activities
    7.
    发明授权
    Sir2 products and activities 有权
    Sir2产品和活动

    公开(公告)号：US07432246B2

    公开(公告)日：2008-10-07

    申请号：US11248523

    申请日：2005-10-12

    申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

    发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

    IPC分类号： C07H19/04 ,  C12P19/36 ,  C07F9/6512 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

    摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

    摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

    In vitro transposition of artificial transposons
    10.
    发明授权
    In vitro transposition of artificial transposons 失效
    人工转座子的体外转座

    公开(公告)号：US5677170A

    公开(公告)日：1997-10-14

    申请号：US204675

    申请日：1994-03-02

    申请人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

    发明人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

    IPC分类号： C12N15/09 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/65 ,  C07H21/04 ,  C12N15/11 ,  C12N15/70

    CPC分类号： C12N15/90 ,  C12Q1/6869 ,  C12Q1/6895

    摘要： We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

    摘要翻译： 我们开发了有效的创建人工转座子的方法，并将这些转座子在体外插入到质粒靶标中，主要用于测序和测序DNA。 已经将工程改造成质粒，将几乎任何DNA序列或序列的组合转化成人工转座子; 因此，可以容易地设计和构造含有任何期望特征的定制转座子。 然后使用存在于酵母Ty1病毒样颗粒中的整合酶活性，将该转座子在体外有效地插入质粒靶标中。 与转座子末端互补的引物可用于对任何转座子插入侧翼的DNA进行序列比对。