公开(公告)号：US5965368A

公开(公告)日：1999-10-12

申请号：US959536

申请日：1997-10-24

申请人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

发明人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N15/10 ,  C12Q1/68 ,  C12R1/865 ,  G01N33/50 ,  G01N33/53 ,  C12N15/81

CPC分类号： G01N33/5023 ,  C12N15/1055 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/505 ,  G01N33/53 ,  G01N2333/39

摘要： Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

公开(公告)号：US07033768B2

公开(公告)日：2006-04-25

申请号：US10027219

申请日：2001-12-21

申请人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

发明人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

IPC分类号： G01N33/53

CPC分类号： G01N33/5023 ,  C12N15/1055 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/505 ,  G01N33/53 ,  G01N2333/39 ,  C12Q2565/201 ,  C12Q2522/101

摘要： Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

公开(公告)号：US5955280A

公开(公告)日：1999-09-21

申请号：US923274

申请日：1997-09-04

申请人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

发明人： Marc Vidal ,  Jef D. Boeke ,  Ed Harlow

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N15/10 ,  C12Q1/68 ,  C12R1/865 ,  G01N33/50 ,  G01N33/53 ,  C07H21/04 ,  C12N5/10

CPC分类号： G01N33/5023 ,  C12N15/1055 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/505 ,  G01N33/53 ,  G01N2333/39

摘要： Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

摘要翻译： 公开了用于鉴定分子相互作用（例如蛋白质/蛋白质，蛋白质/ DNA，蛋白质/ RNA或RNA / RNA相互作用）的方法。 本发明中的所有方法都采用反选择和至少两种杂交分子。 相互作用的分子重构转录因子并直接表达报告基因，然后测定其表达。 还公开了可用于实践本发明的方法的遗传构建体。

公开(公告)号：US07741295B2

公开(公告)日：2010-06-22

申请号：US12231880

申请日：2008-09-05

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

IPC分类号： A61K31/70 ,  A01N43/04

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

公开(公告)号：US5968785A

公开(公告)日：1999-10-19

申请号：US796364

申请日：1997-02-06

申请人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

发明人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

IPC分类号： C12N15/09 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/64 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N15/90 ,  C12Q1/6869 ,  C12Q1/6895

摘要： We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

摘要翻译： 我们开发了有效的创建人工转座子的方法，并将这些转座子在体外插入到质粒靶标中，主要用于测序和测序DNA。 已经将工程改造成质粒，将几乎任何DNA序列或序列的组合转化成人工转座子; 因此，可以容易地设计和构造含有任何期望特征的定制转座子。 然后使用存在于酵母Ty1病毒样颗粒中的整合酶活性，将该转座子在体外有效地插入质粒靶标中。 与转座子末端互补的引物可用于对任何转座子插入侧翼的DNA进行序列比对。

公开(公告)号：US07432246B2

公开(公告)日：2008-10-07

申请号：US11248523

申请日：2005-10-12

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauvé ,  Ivana Celic

IPC分类号： C07H19/04 ,  C12P19/36 ,  C07F9/6512 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。

公开(公告)号：US5677170A

公开(公告)日：1997-10-14

申请号：US204675

申请日：1994-03-02

申请人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

发明人： Scott E. Devine ,  Jef D. Boeke ,  Lelita T. Braiterman

IPC分类号： C12N15/09 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/65 ,  C07H21/04 ,  C12N15/11 ,  C12N15/70

CPC分类号： C12N15/90 ,  C12Q1/6869 ,  C12Q1/6895

摘要： We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.

摘要翻译： 我们开发了有效的创建人工转座子的方法，并将这些转座子在体外插入到质粒靶标中，主要用于测序和测序DNA。 已经将工程改造成质粒，将几乎任何DNA序列或序列的组合转化成人工转座子; 因此，可以容易地设计和构造含有任何期望特征的定制转座子。 然后使用存在于酵母Ty1病毒样颗粒中的整合酶活性，将该转座子在体外有效地插入质粒靶标中。 与转座子末端互补的引物可用于对任何转座子插入侧翼的DNA进行序列比对。

公开(公告)号：US4595658A

公开(公告)日：1986-06-17

申请号：US416922

申请日：1982-09-13

申请人： Norman D. Zinder ,  Peter Model ,  Jef D. Boeke

发明人： Norman D. Zinder ,  Peter Model ,  Jef D. Boeke

IPC分类号： C07K14/005 ,  C12N15/62 ,  C12N15/70 ,  C12P21/00 ,  C12N1/00 ,  C12N1/02 ,  C12N1/20 ,  C12N15/00 ,  C12P21/02 ,  C12P21/04 ,  C12P21/06 ,  C12R1/19

CPC分类号： C07K14/005 ,  C12N15/625 ,  C12N15/70 ,  C07K2319/035 ,  C12N2795/14122 ,  Y10S435/849

摘要： A method of externalizing proteins from the periplasmic space of gram-negative bacteria and in particular, E. coli and its relatives, comprising utilizing bacteria which have a phage gene, coding for a protein (such as gene III protein) or a mutant bacterial gene (such as a gene coding for a membrane function) which causes perturbation of the outer bacterial membrane resulting in leakage of proteins in the periplasmic space from the cell.

摘要翻译： 包括利用具有噬菌体基因的细菌编码蛋白质（例如基因III蛋白质）或突变型细菌基因的革兰氏阴性细菌，特别是大肠杆菌及其亲属的周质空间的外源化蛋白质的方法 （例如编码膜功能的基因），其引起外部细菌膜的扰动，导致周质空间中的蛋白质从细胞泄漏。

公开(公告)号：US20110257086A1

公开(公告)日：2011-10-20

申请号：US13122438

申请日：2009-09-18

申请人： Philip A. Cole ,  Bradley P. Barnett ,  Yousang Hwang ,  Jef D. Boeke

发明人： Philip A. Cole ,  Bradley P. Barnett ,  Yousang Hwang ,  Jef D. Boeke

IPC分类号： A61K38/16 ,  C07K7/08 ,  A61P1/00 ,  C07K1/107 ,  A61P3/10 ,  A61P3/04 ,  C07K14/00 ,  A61K38/10

CPC分类号： C07K14/60 ,  A61K38/00 ,  A61K47/645

摘要： The invention provides inhibitors of ghrelin O-acyltransferase, and methods of making and using them. In some embodiments, the invention provides bisubstrate analog inhibitors of ghrelin O-acyltransferase, which can be effective in treating, for example, obesity and diabetes mellitus.

摘要翻译： 本发明提供生长素释放肽O-酰基转移酶的抑制剂及其制备和使用方法。 在一些实施方案中，本发明提供了生长素释放肽O-酰基转移酶的双底物类似物抑制剂，其可有效治疗例如肥胖症和糖尿病。

公开(公告)号：US20090068695A1

公开(公告)日：2009-03-12

申请号：US12231880

申请日：2008-09-05

申请人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauve ,  Ivana Celic

发明人： Vern L. Schramm ,  Jef D. Boeke ,  Anthony Sauve ,  Ivana Celic

IPC分类号： C12Q1/34

CPC分类号： C12N9/1077 ,  C07H19/04 ,  C07H19/20 ,  C12N9/99 ,  C12P19/32

摘要： A novel compound, 2′/3′-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2′ and 3′ regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2′-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD+. Analogs of 2′/3′-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2′/3′-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2′/3′-O-acetyl-ADP-ribose are also provided.

摘要翻译： 提供新的化合物2'/ 3'-O-乙酰基-ADP-核糖。 该化合物是O-乙酰基-ADP核糖的2'和3'区域异构体的混合物，并且由2'-O-乙酰基-ADP-核糖非酶催化形成，其是Sir2酶与 乙酰化肽和NAD +。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的类似物。 另外，制备2'/ 3'-O-乙酰基-ADP-核糖的方法，确定测试化合物是否为Sir2酶抑制剂的方法，组合物中Sir2活性检测方法，乙酰化肽脱乙酰化的方法， 并提供了抑制乙酰化肽脱乙酰化的方法。 还提供了2'/ 3'-O-乙酰基-ADP-核糖的前药。