公开(公告)号：US20090035826A1

公开(公告)日：2009-02-05

申请号：US12181358

申请日：2008-07-29

申请人： Jeffrey S. Tolan ,  Brian Foody ,  Stephen Rowland

发明人： Jeffrey S. Tolan ,  Brian Foody ,  Stephen Rowland

IPC分类号： C12P19/14 ,  C12P7/02 ,  C12P7/16 ,  C12P7/10

CPC分类号： C07H3/02 ,  B01D3/001 ,  C07K14/195 ,  C08H8/00 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/582

摘要： A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.

摘要翻译： 提供了从预处理的木质纤维素原料生产葡萄糖的方法。 该方法包括用纤维素酶酶促水解预处理的木质纤维素原料，以产生包含葡萄糖和未水解的纤维素的水解产物浆液，并在发酵反应中发酵水解产物浆料以产生包含醇的发酵液。 得到包含未水解纤维素的工艺流，然后将其进行变性步骤，优选包括将未水解的纤维素暴露于升高的温度，从而产生包含未水解纤维素的热处理流。 然后将含有未水解纤维素的经热处理的料流进一步用纤维素酶水解，以将纤维素水解成葡萄糖。

公开(公告)号：US08980599B2

公开(公告)日：2015-03-17

申请号：US12181358

申请日：2008-07-29

申请人： Jeffrey S. Tolan ,  Brian Foody ,  Stephen Rowland

发明人： Jeffrey S. Tolan ,  Brian Foody ,  Stephen Rowland

IPC分类号： C12P7/10 ,  C12P19/14 ,  C12P7/02 ,  C12P7/06 ,  C12P7/08 ,  C07K14/00 ,  C07H3/02 ,  B01D3/00 ,  C08H8/00 ,  C12P19/02 ,  C13K1/02 ,  C07K14/195

CPC分类号： C07H3/02 ,  B01D3/001 ,  C07K14/195 ,  C08H8/00 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/582

摘要： A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.

摘要翻译： 提供了从预处理的木质纤维素原料生产葡萄糖的方法。 该方法包括用纤维素酶酶促水解预处理的木质纤维素原料，以产生包含葡萄糖和未水解的纤维素的水解产物浆液，并在发酵反应中发酵水解产物浆料以产生包含醇的发酵液。 得到包含未水解纤维素的工艺流，然后将其进行变性步骤，优选包括将未水解的纤维素暴露于升高的温度，从而产生包含未水解纤维素的热处理流。 然后将含有未水解纤维素的经热处理的料流进一步用纤维素酶水解，以将纤维素水解成葡萄糖。

公开(公告)号：US20120209028A1

公开(公告)日：2012-08-16

申请号：US13392744

申请日：2010-08-18

申请人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand

发明人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand

IPC分类号： C07C51/42

CPC分类号： C12F3/00 ,  C07C51/43 ,  C07C51/445 ,  Y02E50/16 ,  C07C53/08

摘要： A process for recovering a volatile carboxylic acid from an aqueous stream comprising same, the process comprising the steps of: (i) steam stripping the carboxylic acid from the aqueous stream, which aqueous stream is produced by a conversion process using a lignocellulosic feedstock as a substrate, the steam stripping comprising contacting the aqueous stream with steam by flowing the aqueous stream and the steam countercurrent to one another, thereby producing a vapour stream comprising vapourized carboxylic acid and steam and a stripped aqueous stream; (ii) extracting the vapourized carboxylic acid with an organic solvent by contacting the vapour stream with the organic solvent to produce (a) a stream comprising the organic solvent and the carboxylic acid and (b) the steam at least substantially depleted of the carboxylic acid, wherein the organic solvent has an atmospheric boiling point of at least about 150° C. and is insoluble in water; (iii) returning the steam from step (ii) to the steam stripping step (step i) to further strip the carboxylic acid from the aqueous stream; and (iv) separating the carboxylic acid from the organic solvent.

摘要翻译： 一种从包含其的含水流中回收挥发性羧酸的方法，该方法包括以下步骤：（i）从水流中汽提汽提羧酸，该水流通过使用木质纤维素原料的转化方法生产， 蒸汽汽提包括通过使水流和蒸汽逆流地相互流动使水流与蒸汽接触，从而产生包含蒸发的羧酸和蒸汽和汽提的水流的蒸气流; （ii）通过使蒸气流与有机溶剂接触来提取挥发的羧酸，以产生（a）包含有机溶剂和羧酸的物流，和（b）至少基本上不含羧酸的蒸汽 其中所述有机溶剂的常压沸点为至少约150℃，并且不溶于水; （iii）将来自步骤（ii）的蒸汽返回到汽提步骤（步骤i），以进一步从水流中分离出羧酸; 和（iv）从有机溶剂中分离出羧酸。

公开(公告)号：US20100221819A1

公开(公告)日：2010-09-02

申请号：US12762589

申请日：2010-04-19

申请人： Brian Foody ,  Ziyad Rahme ,  Jeffrey S. Tolan

发明人： Brian Foody ,  Ziyad Rahme ,  Jeffrey S. Tolan

IPC分类号： C12M1/00

CPC分类号： C12P19/14 ,  C12M45/09 ,  C12P19/02 ,  C12P19/04 ,  Y02E50/16 ,  Y02E50/17

摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided.

摘要翻译： 提供了一种纤维素酶水解从预处理的纤维素原料产生水解产物的方法。 该方法包括在水解反应器的底部引入预处理的纤维素原料的含水浆料。 通过避免混合并保持约0.1至约20英尺/小时的平均浆料流速，使得未溶解的固体以比液体慢的速率向上流动，来限制反应器中的轴向分散。 在引入步骤之前或期间，将纤维素酶加入到含水浆料中。 从水解反应器中除去包含水解产物和未水解固体的水流。 还提供了包含用于该方法的纤维素酶和絮凝剂的酶组合物。 另外，提供了包含纤维素酶和絮状物的试剂盒。

公开(公告)号：US20100184151A1

公开(公告)日：2010-07-22

申请号：US12686485

申请日：2010-01-13

申请人： Jeffrey S. Tolan ,  Brian Foody

发明人： Jeffrey S. Tolan ,  Brian Foody

IPC分类号： C12P19/00 ,  C12P7/04

CPC分类号： C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

摘要： A method for producing glucose from a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock with acid to produce a pretreated feedstock composition. A calcium-containing stream is provided that comprises calcium that is obtained from the lignocellulosic feedstock and a calcium carbonate-containing stream is obtained by precipitation of the calcium from the calcium-containing stream. The pH of the pretreated feedstock is adjusted with (a) the calcium carbonate-containing stream; (b) a calcium hydroxide-containing stream that is derived from said calcium carbonate-containing stream by subjecting said calcium carbonate-containing stream to calcination; or (c) a combination of the calcium carbonate-containing stream and the calcium hydroxide-containing stream. The pH adjustment results in a neutralized pretreated lignocellulosic feedstock having a pH between about 3 and about 9 and enzymatic hydrolysis of the neutralized, pretreated lignocellulosic feedstock is then conducted with cellulase enzymes to produce the glucose.

摘要翻译： 提供了一种从木质纤维原料生产葡萄糖的方法。 该方法包括用酸处理木质纤维素原料以产生预处理的原料组合物。 提供含钙流，其包含从木质纤维素原料获得的钙，并且通过从含钙流中沉淀钙获得含碳酸钙的流。 用（a）含碳酸钙的料流调节预处理原料的pH; （b）通过使所述含碳酸钙的料流进行煅烧从含有所述含碳酸钙的料流中得到的含氢氧化钙的料流; 或（c）含碳酸钙的料流和含氢氧化钙的料流的组合。 pH调节导致pH为约3至约9的中和预处理木质纤维素原料，然后用纤维素酶进行中和的预处理的木质纤维素原料的酶水解以产生葡萄糖。

公开(公告)号：US6090595A

公开(公告)日：2000-07-18

申请号：US207468

申请日：1998-12-08

申请人： Brian Foody ,  Jeffrey S. Tolan ,  Jerome D. Bernstein ,  Patrick Foody, Sr.

发明人： Brian Foody ,  Jeffrey S. Tolan ,  Jerome D. Bernstein ,  Patrick Foody, Sr.

IPC分类号： C12P7/10 ,  C12P19/14 ,  C12P7/06 ,  C12P7/14

CPC分类号： C12P7/10 ,  Y02E50/16

摘要： An improved pretreatment of cellulosic feedstocks, to enable economical ethanol production by enzyme treatment. The improved pretreatment comprises choosing either a feedstock with a ratio of arabinoxylan to total nonstarch polysaccharides (AX/NSP) of greater than about 0.39, or a selectively bred feedstock on the basis of an increased ratio of AX/NSP over a starting feedstock material, and reacting at conditions that disrupt the fiber structure and hydrolyze a portion of the cellulose and hemicellulose. This pretreatment produces a superior substrate for enzymatic hydrolysis, by enabling the production of more glucose with less cellulose enzyme than any known procedures. This pretreatment is uniquely suited to ethanol production. Preferred feedstocks with an AX/NSP level greater than about 0.39 include varieties of oat hulls and corn cobs.

摘要翻译： 改进纤维素原料的预处理，以通过酶处理实现经济的乙醇生产。 改进的预处理包括选择具有大于约0.39的阿拉伯木聚糖与总非淀粉多糖（AX / NSP）的比例的原料或基于增加的起始原料材料的AX / NSP比例的选择性培育的原料， 并在破坏纤维结构并水解一部分纤维素和半纤维素的条件下进行反应。 该预处理通过使得能够以比任何已知方法更少的纤维素酶产生更多的葡萄糖来产生用于酶水解的优异底物。 这种预处理特别适用于乙醇生产。 AX / NSP水平大于约0.39的优选原料包括燕麦壳和玉米芯的品种。

公开(公告)号：US20120329096A1

公开(公告)日：2012-12-27

申请号：US13569308

申请日：2012-08-08

申请人： Brian Foody ,  Jeffrey S. Tolan ,  Ziyad Rahme ,  Vijay Anand

发明人： Brian Foody ,  Jeffrey S. Tolan ,  Ziyad Rahme ,  Vijay Anand

IPC分类号： C12P7/10 ,  C12P19/00

CPC分类号： B01D61/147 ,  B01B1/005 ,  B01D15/1821 ,  B01D15/365 ,  B01D61/44 ,  C01C1/28 ,  C01D3/04 ,  C01D5/04 ,  C01D5/16 ,  C05B7/00 ,  C05C3/00 ,  C05D1/00 ,  C05D1/02 ,  C05D5/00 ,  C05F11/00 ,  C12P3/00 ,  C12P7/06 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  Y02E50/16 ,  Y02E50/17

摘要： A method for recovering inorganic salt during processing of a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock by adding an acid to the feedstock to produce a pretreated lignocellulosic feedstock. A soluble base is then added to the pretreated lignocellulosic feedstock to adjust the pH and produce a neutralized feedstock. The neutralized feedstock is then enzymatically hydrolyzed to produce an enzyme hydrolyzed feedstock and a sugar stream. Inorganic salt is recovered from either a stream obtained from the lignocellulosic feedstock prior to the step of pretreating, a stream obtained from the pretreated lignocellulosic feedstock, a stream obtained from the neutralized feedstock, a stream obtained from the sugar stream, or a combination of these streams. The inorganic salt may be concentrated, clarified, recovered and purified by crystallization, electrodialysis drying, or agglomeration and granulation, and then used as desired, for example as a fertilizer.

摘要翻译： 提供了在木质纤维素原料的加工过程中回收无机盐的方法。 该方法包括通过向原料中加入酸来预处理木质纤维素原料以产生预处理的木质纤维素原料。 然后将可溶碱添加到预处理的木质纤维素原料中以调节pH并产生中和的原料。 然后将中和的原料酶水解以产生酶水解原料和糖流。 在预处理步骤之前从木质纤维素原料获得的物流中回收无机盐，从预处理的木质纤维素原料获得的物流，从中和原料获得的物流，从糖流获得的物流，或这些物质的组合 流。 无机盐可以通过结晶，电渗析干燥或附聚和造粒进行浓缩，澄清，回收和纯化，然后根据需要使用，例如作为肥料。

公开(公告)号：US20120215026A1

公开(公告)日：2012-08-23

申请号：US13392728

申请日：2010-08-18

申请人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand ,  Daphne Wahnon

发明人： Jeffrey S. Tolan ,  Brian Foody ,  Vijay Anand ,  Daphne Wahnon

IPC分类号： C07C51/44

CPC分类号： B01D3/02 ,  B01D3/002 ,  C07C51/445 ,  C12F3/00 ,  Y02E50/16 ,  C07C53/08

摘要： A process for recovering a volatile carboxylic acid from an aqueous stream containing same comprising the steps of: (a) evaporating the aqueous stream to produce a vapour stream comprising the volatile carboxylic acid that has been vapourized and water vapour, which aqueous stream is produced by a conversion process using a lignocellulosic feedstock as a substrate; (b) contacting the vapour stream with an organic solvent so as to extract the volatile carboxylic acid present in the vapour stream, thereby producing a liquid stream comprising the organic solvent and the volatile carboxylic acid, and a water vapour stream, wherein the organic solvent has an atmospheric boiling point of at least about 150° C. and is insoluble in water; and (c) separating the volatile carboxylic acid from the organic solvent.

摘要翻译： 一种从含有它的含水物流中回收挥发性羧酸的方法，包括以下步骤：（a）蒸发含水物流以产生蒸气流，该蒸汽流包含已蒸发的挥发性羧酸和水蒸汽，该水流由 使用木质纤维素原料作为底物的转化方法; （b）使蒸气流与有机溶剂接触以提取存在于蒸气流中的挥发性羧酸，从而产生包含有机溶剂和挥发性羧酸的液体流以及水蒸气流，其中有机溶剂 具有至少约150℃的常压沸点，并且不溶于水; 和（c）从有机溶剂中分离挥发性羧酸。

公开(公告)号：US20090023187A1

公开(公告)日：2009-01-22

申请号：US11658338

申请日：2005-07-15

申请人： Brian Foody ,  Jeffrey S. Tolan

发明人： Brian Foody ,  Jeffrey S. Tolan

IPC分类号： C12P19/00 ,  C12P7/10 ,  C12P7/56 ,  C13K1/02

CPC分类号： C12P7/56 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16

摘要： A process for obtaining a product sugar stream from cellulosic biomass is disclosed. In one process, the cellulosic biomass is pretreated at a pH between about 0.4 to 2.0 by adding one or more than one acid to produce a pretreated cellulosic biomass comprising acetic acid. One or more than one base is then added to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0 to produce a neutralized cellulosic biomass comprising inorganic salt and acetate salt. The neutralized biomass is then hydrolyzed by cellulase enzymes to produce a crude sugar stream. Insoluble residue is separated from the crude sugar stream and the resulting clarified sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more raffinate streams and a product stream. The raffinate stream comprises inorganic salts and acetate salts, and the product stream comprises sugar. The product stream may then be fermented or otherwise further processed. In an alternate process, a product sugar stream is obtained from a crude sugar stream that is produced from conversion of cellulosic biomass to sugar. The cellulosic biomass may be produced using any suitable method. In this process the crude sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, and the product sugar stream is obtained.

摘要翻译： 公开了一种从纤维素生物质获得产品糖流的方法。 在一个过程中，纤维素生物质在约0.4至2.0的pH下通过加入一种或多种酸预处理以产生包含乙酸的预处理的纤维素生物质。 然后将一个或多于一个碱加入到预处理的纤维素生物质中以将预处理的纤维素生物质调节至约4.0至约6.0的pH，以产生包含无机盐和乙酸盐的中和的纤维素生物质。 然后中和的生物质被纤维素酶水解以产生粗糖流。 将不溶性残余物与粗糖流分离，并使用约pH 5.0至约10.0的离子排斥色谱处理所得澄清的糖流，以产生一个或多个萃余液流和产物流。 萃余液流包含无机盐和乙酸盐，产物流包含糖。 然后可以将产物流发酵或进一步加工。 在替代方法中，从由纤维素生物质转化为糖产生的粗糖流获得产物糖流。 可以使用任何合适的方法制备纤维素生物质。 在该方法中，使用约pH 5.0至约10.0的离子排斥色谱法处理粗糖流，以产生一种或多于一种包含硫酸盐和乙酸盐的萃余液流和包含糖的产物流，并获得产物糖流。

公开(公告)号：US07419809B2

公开(公告)日：2008-09-02

申请号：US10381442

申请日：2001-09-25

申请人： Brian Foody ,  Theresa C. White ,  Jeffrey S. Tolan ,  Jennifer Donaldson

发明人： Brian Foody ,  Theresa C. White ,  Jeffrey S. Tolan ,  Jennifer Donaldson

IPC分类号： C12P1/00 ,  C12P19/00 ,  C12P19/02 ,  C12P19/14 ,  C12N9/00

CPC分类号： C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01091

摘要： The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and a modified CBHO, wherein the modified CBHI is present in the enzyme mixture at an amount relative to all CBHI-type enzymes from about 15% to about 100% (w/w),depending upon the modified CBHI used. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal dedicated ethanol crops forestry products, and pulp and paper products, or combinations thereof.

摘要翻译： 本发明涉及通过用包含纤维素酶和修饰的CBHO的酶混合物处理预处理的木质纤维素底物来将纤维素转化为葡萄糖的方法，其中所述修饰的CBHI以相对于所有CBHI型酶的量存在于酶混合物中 约15％至约100％（w / w），这取决于所使用的改性CBHI。 预处理的木质纤维素底物选自农业残留物，淀粉或糖去除后的残留物专用乙醇作物林业产品，纸浆和纸制品或其组合。