摘要:
The present invention discloses a cover including a base, a patterned layer, a protective layer and a bottom layer. The base has a first surface, and a second surface on the opposite side of the first surface. The patterned layer is formed on the first surface of the base. The protective layer and the bottom layer are integrally formed on the second surface of the base and on the patterned layer respectively by injection molding. The present invention also provides a method for manufacturing a cover. The present cover can tend give a various and vivid appearances whilst also having good performance.
摘要:
A minimal-copy-ratio of templates is a problem in detecting early stage cancer where minimal copies of somatic cancer-specific mutations are targeted in the presence of large copies of wildtype genome DNA, commonly a 1/10,000 or even less minimal-copy-ratios between the mutant target and wildtype control templates. To overcome this problem, delayed pyrophosphorolysis activated polymerization (delayed-PAP) was developed which can delay product accumulation of the wildtype control to a much later time or cycle, such as by 15 cycles or by 30,000 folds. In the multiplex format, delayed-PAP is particularly useful to amplify not only the wildtype control but also mutant target templates accurately and consistently in the minimal-copy-ratio situation.
摘要:
A new fluorescence detection method called pyrophosphorolysis activated fluorescence was developed to measure PAP amplification of nucleic acid. A fluorophore-quencher dual-attached blocked primer was used for PAP which has a fluorophore attached to a nucleotide in the internal region or at the 5′ end and a quencher attached to a blocked nucleotide at the 3′ end. Multiple fluorophore-quencher dual-labeled blocked primers were also used for multiplex PAP, which are attached with different fluorophores to distinguish multiple templates in a reaction.
摘要:
Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.
摘要:
The invention provides a method for lyophilizing integrated composition of pyrophosphorolysis activated polymerization (PAP) in an aqueous solution. It also provides lyophilized integrated PAP composition. Except for nucleic acid template, the integrated composition contains all components. For manipulation, simply add nucleic acid template in an aqueous solution to start amplification. In addition to the easy manipulation, the lyophilized integrated composition can be stored for prolonged period at ambiguous temperature.
摘要:
Wind turbine blades with layered, multi-component spars, and associated systems and methods are disclosed. A wind turbine blade system in accordance with a particular embodiment includes a first blade segment having a first spar element that includes first planks having a first thickness and a first plank composition, and a second blade segment having a second spar element that includes second planks having a second thickness and a second plank composition different than the first plank composition. The second blade segment is joined to the first blade segment at a joint, and, in particular embodiments, an overall product of thickness and elastic modulus of the first planks is approximately equal to an overall product of thickness and elastic modulus for the second planks.
摘要:
A method for scheduling communication traffic in an Advanced Telecom Computing Architecture based equipment and a corresponding apparatus are provided. In the method, communication traffic information of traffic processing means in the equipment is collected; based on the collected communication traffic information, a traffic distribution rule for the traffic processing means in next time period is generated; and based on the generated traffic distribution rule, incoming communication traffic of the equipment is scheduled. The method of the invention considers the quality of service and contracted capacity of the whole equipment and the dynamic processing capability and running environment of the traffic processing means, and can balance the loads of the respective traffic processing means in the equipment with asymmetric multi-application configuration well by adaptively adjusting the distribution of the communication traffic, to guarantee the quality of service of the whole equipment.
摘要:
The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane. Upon flowing a nucleic acid-containing solution through the first anion exchange membrane, the nucleic acid binds to and then elutes from the first membrane. The eluted solution then flows serially through the second silica membrane, which the nucleic acid binds to and then elutes from. Due to this novel serial coupled double-layer principle, the present invention is particularly suitable for co-isolating RNA and DNA, for isolating nucleic acid embraced by proteins, e.g., viruses, and for isolating diluted nucleic acid in a large volume, e.g., plasma. In addition, the eluted nucleic acid is ready for downstream applications.
摘要:
A new method of RNA-PAP was developed that can directly amplify RNA template without additional treatment. RNA-PAP brings in a new mechanism for amplification of RNA template in which RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization are serially coupled using 3′ blocked primers. Due to this serial coupling, RNA-PAP has high selectivity against mismatches on the RNA template, providing highly specific amplification of RNA template. In addition, mutant polymerases were genetically engineered for higher efficiency of RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization.
摘要:
A data processing system and method are disclosed. The system comprises an instruction-fetch stage where an instruction is fetched and a specific instruction is input into decode stage; a decode stage where said specific instruction indicates that contents of a register in a register file are used as an index, and then, the register file pointed to by said index is accessed based on said index; an execution stage where an access result of said decode stage is received, and computations are implemented according to the access result of the decode stage.