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    Polypeptides having xylanase activity from Aspergillus fumigatus
    7.
    发明授权
    Polypeptides having xylanase activity from Aspergillus fumigatus 有权
    具有来自烟曲霉的木聚糖酶活性的多肽

    公开(公告)号：US07960160B2

    公开(公告)日：2011-06-14

    申请号：US11054191

    申请日：2005-02-09

    申请人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

    发明人： Debbie Yaver ,  Paul Harris ,  Suzanne Otani ,  Janine Lin ,  Haiyan Ge

    IPC分类号： C12N9/24 ,  C12N15/56

    CPC分类号： C12N9/2482 ,  C12N9/248 ,  C12Y302/01008

    摘要： The present invention relates to an isolated polypeptide having xylanase activity from Aspergillus fumigatus with a molecular weight of approximately 50 kDa by SDS-PAGE and homologous polypeptides thereof comprising an amino acid sequence having at least 95% identity with amino adds 20 to 397 of SEQ ID NO: 6; encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with nucleotides 107 to 1415 of SEQ ID NO: 5: or encoded by a polynucleotide that hybridizes under high stringency conditions with (i) nucleotides 107 to 1415 of SEQ ID NO: 5, (ii) the cDNA sequence of (i), or (iii) a full-length complementary strand of (i) or (ii). The present invention also relates to a method for producing such polypeptides having xylanase activity comprising: (a) cultivating a wild-type Aspergillus fumigatus cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

    摘要翻译： 本发明涉及通过SDS-PAGE分析重量为约50kDa的烟曲霉具有木聚糖酶活性的分离多肽，并且其同源多肽包含与氨基具有至少95％同一性的氨基酸序列，添加SEQ ID NO：20至397 NO：6; 由包含与SEQ ID NO：5的核苷酸107至1415具有至少95％同一性的核苷酸序列的多核苷酸编码，或由在高严格条件下与（i）SEQ ID NO：5的核苷酸107至1415杂交的多核苷酸编码。 5，（ii）（i）或（iii）（i）或（ii）的全长互补链的cDNA序列。 本发明还涉及生产具有木聚糖酶活性的多肽的方法，其包括：（a）在有利于产生多肽的条件下培养野生型烟曲霉细胞; 和（b）回收多肽。