公开(公告)号：US20100192308A1

公开(公告)日：2010-08-05

申请号：US12667577

申请日：2008-06-16

申请人： Jian Chen ,  Guocheng Du ,  Hejing Yan ,  Jianghua Li

发明人： Jian Chen ,  Guocheng Du ,  Hejing Yan ,  Jianghua Li

IPC分类号： D06L3/14

CPC分类号： D06M16/003 ,  D06L4/40 ,  D06L4/75 ,  D06M2101/06

摘要： A method for scouring and bleaching cotton fabric with composite enzyme preparation in one bath is provided. The method relates to the application of composite alkaline enzyme preparation in scouring and bleaching processes of cotton fabric. The enzyme preparation for scouring and bleaching cotton fabric is compounded from cutinase, alkaline pectase, alkaline xylanase, alkaline cellulose, and carbohydrate oxidase. The method includes: scouring the cotton fabric by adding the composite enzyme preparation, glucose, oxidation bleaching stabilizer RB-3, penetrant JFC, and Triton X-100 in a treating bath at 55-70° C. and pH 8-10, and then bleaching the cotton fabric for 30 min by directly adding tetraacetyl ethylenediamine (TAED) in the scouring bath to activate hydrogen peroxide. After being treated by the method of the present invention, the cotton fabric has high impurity removal ratio, favorable wettability, and high whiteness. The present invention has the advantages of low power consumption, short process flow, and being environment friendly, and may be used to replace conventional chemical treatment process under the condition of concentrated alkali and high temperature.

摘要翻译： 提供了在一个浴中用复合酶制剂冲洗和漂白棉织物的方法。 该方法涉及复合碱性酶制剂在棉织物的漂洗和漂白过程中的应用。 用于冲洗和漂白棉织物的酶制剂由角质酶，碱性果胶酶，碱性木聚糖酶，碱性纤维素和碳水化合物氧化酶组合。 该方法包括：通过在55-70℃和pH8-10的处理浴中加入复合酶制剂，葡萄糖，氧化漂白稳定剂RB-3，渗透剂JFC和Triton X-100来洗涤棉织物，以及 然后通过在洗涤浴中直接加入四乙酰乙二胺（TAED）来活化过氧化氢，使棉织物漂白30分钟。 在通过本发明的方法处理之后，棉织物具有高杂质去除率，良好的润湿性和高白度。 本发明具有功耗低，工艺流程短，环保的优点，可用于在浓碱和高温条件下代替常规化学处理工艺。

公开(公告)号：US08722364B2

公开(公告)日：2014-05-13

申请号：US13228572

申请日：2011-09-09

申请人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

发明人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

IPC分类号： C12P21/04

CPC分类号： C12N9/0065 ,  C12N1/20

摘要： Disclosed are methods for increasing microbial catalase production. 1-10 g/L sodium hexametaphosphate was added to the culture medium between 30-40 hours of fermentation to inhibit proteinase activity and increase the production of catalase. This simple modification of fermentation procedure can result in up to 45% increase of the production of catalase.

摘要翻译： 公开了增加微生物过氧化氢酶产生的方法。 在发酵30-40小时之间向培养基中加入1-10g / L六偏磷酸钠以抑制蛋白酶活性并增加过氧化氢酶的产生。 发酵过程的这种简单的修改可以导致过氧化氢酶的产量提高高达45％。

公开(公告)号：US09187773B2

公开(公告)日：2015-11-17

申请号：US14047968

申请日：2013-10-07

申请人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

发明人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

IPC分类号： C12P7/52

CPC分类号： C12P7/52

摘要： The invention provides a simple and effective method for improving acid tolerance of P. acidipropionici by adding arginine and/or aspartic acid to the culture medium. The acid tolerance of P. acidipropionici was improved by 60% and 20% respectively through adding arginine or aspartic acid into the culture medium. Consequently, PA production was improved by 36% and 26%, respectively. The maximal PA production was obtained by adding both 20 mM arginine and 20 mM aspartic acid. This method can be applied to large scale production of PA.

摘要翻译： 本发明提供了一种通过向培养基中加入精氨酸和/或天冬氨酸来改善酸性丙酸酸的耐酸性的简单和有效的方法。 通过向培养基中加入精氨酸或天冬氨酸，分别将酸性丙酸的耐酸性提高了60％和20％。 因此，PA产量分别提高了36％和26％。 通过加入20mM精氨酸和20mM天冬氨酸获得最大的PA产生。 该方法可应用于大规模生产PA。

公开(公告)号：US20140178952A1

公开(公告)日：2014-06-26

申请号：US14047968

申请日：2013-10-07

申请人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

发明人： Jian Chen ,  Guocheng Du ,  Long Liu ,  Jianghua Li ,  Ningzi Guan

IPC分类号： C12P7/52

CPC分类号： C12P7/52

摘要： The invention provides a simple and effective method for improving acid tolerance of P. acdipropionici by adding arginine and/or aspartic acid to the culture medium. The acid tolerance of P. acdipropionici was improved by 60% and 20% respectively through adding arginine or aspartic acid into the culture medium. Consequently, PA production was improved by 36% and 26%, respectively. The maximal PA production was obtained by adding both 20 mM arginine and 20 mM aspartic acid. This method can be applied to large scale production of PA.

摘要翻译： 本发明提供了一种简单有效的方法，通过向培养基中加入精氨酸和/或天冬氨酸来改善丙酸二氢吡啶的耐酸性。 通过向培养基中加入精氨酸或天冬氨酸，分别提高了丙酰丙酸的耐酸性分别提高了60％和20％。 因此，PA产量分别提高了36％和26％。 通过加入20mM精氨酸和20mM天冬氨酸获得最大的PA产生。 该方法可应用于大规模生产PA。

公开(公告)号：US20130065292A1

公开(公告)日：2013-03-14

申请号：US13228572

申请日：2011-09-09

申请人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

发明人： Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Long Liu ,  Zhuolin Feng

IPC分类号： C12N9/08 ,  C12N1/20

CPC分类号： C12N9/0065 ,  C12N1/20

摘要： Disclosed are methods for increasing microbial catalase production. 1-10 g/L sodium hexametaphosphate was added to the culture medium between 30-40 hours of fermentation to inhibit proteinase activity and increase the production of catalase. This simple modification of fermentation procedure can result in up to 45% increase of the production of catalase.

摘要翻译： 公开了增加微生物过氧化氢酶产生的方法。 在发酵30-40小时之间向培养基中加入1-10g / L六偏磷酸钠以抑制蛋白酶活性并增加过氧化氢酶的产生。 发酵过程的这种简单的修改可以导致过氧化氢酶的产量提高高达45％。

公开(公告)号：US20170009267A1

公开(公告)日：2017-01-12

申请号：US14934166

申请日：2015-11-06

申请人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

发明人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

IPC分类号： C12P19/26 ,  C12N15/75

CPC分类号： C12P19/26 ,  C12N15/75 ,  C12R1/125

摘要： The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

摘要翻译： 本发明提供了通过使用具有glcK敲除的重组枯草芽孢杆菌来增强N-乙酰葡糖胺产生的方法。 本发明通过敲除编码葡糖激酶的glcK基因来增强GlcNAc的产生，从而消除GlcNAc磷酸化成GlcNAc-6-P。 具有glcK敲除的重组枯草芽孢杆菌上清液中GlcNAc的比生长速率和含量分别为0.15h-1和3.0g / L，分别为没有glcK敲除的对照菌株的2.32倍和2.14倍。 本发明的重组枯草芽孢杆菌可能用于GlcNAc的工业生产。

公开(公告)号：US09914949B2

公开(公告)日：2018-03-13

申请号：US14934166

申请日：2015-11-06

申请人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

发明人： Long Liu ,  Jian Chen ,  Guocheng Du ,  Jianghua Li ,  Yanfeng Liu ,  Hannes Link ,  Uwe Sauer

IPC分类号： C12N15/75 ,  C12P19/26

CPC分类号： C12P19/26 ,  C12N15/75 ,  C12R1/125

摘要： The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h−1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

公开(公告)号：US08671097B2

公开(公告)日：2014-03-11

申请号：US12516868

申请日：2007-09-29

申请人： Weixun Wu ,  Jianghua Li ,  Jinyin Zhang ,  Ang Li

发明人： Weixun Wu ,  Jianghua Li ,  Jinyin Zhang ,  Ang Li

IPC分类号： G06F7/00

CPC分类号： G06F11/3476 ,  G06F11/3409 ,  G06F11/3438 ,  G06F11/3452 ,  H04L63/1425 ,  H04L67/22

摘要： The present invention discloses a method and a system for log file analysis based on distributed computing network. The method includes: storing user identifiers and related log information into a log file; dividing the log file into target files each including the log information having the same user identifier; separately analyzing the target files to obtain analysis results using at least two nodes; and combining the analysis results of the nodes. The method thereby establishes relationships among various log files through user identifiers, and further analyzes the relationships among the user's accesses to various contents of a website.

摘要翻译： 本发明公开了一种基于分布式计算网络的日志文件分析方法和系统。 该方法包括：将用户标识符和相关日志信息存储到日志文件中; 将日志文件分为目标文件，每个目标文件包括具有相同用户标识符的日志信息; 使用至少两个节点分别分析目标文件以获得分析结果; 并结合节点的分析结果。 因此，该方法通过用户标识符建立各种日志文件之间的关系，并进一步分析用户对网站各种内容的访问之间的关系。

公开(公告)号：US08463822B2

公开(公告)日：2013-06-11

申请号：US12158689

申请日：2008-03-28

申请人： Jianghua Li ,  Weixun Wu ,  Song Chen

发明人： Jianghua Li ,  Weixun Wu ,  Song Chen

IPC分类号： G06F17/30

CPC分类号： G06F9/5027 ,  G06F2209/5017

摘要： A data merging method for distributed computing uses a configuration file to guide the insertion of computational results obtained by distributed nodes into a database table, and to merge the computational results inserted in the database table. The configuration file is established according to the task splitting conditions of distributed computing. The method uses a data merging server to import and host the configuration file and create a database table corresponding to the configuration file. Each distributed node inserting its distributed computational result into the database table after completing the respective distributed computing task, while the data merging server merges the data in the database table. An interface standard applicable to various uses and various distributed computing tasks may be used such that the user only needs to establish a job-specific configuration file based on the interface standard.

摘要翻译： 用于分布式计算的数据合并方法使用配置文件来指导将由分布式节点获得的计算结果插入到数据库表中，并且合并插入到数据库表中的计算结果。 根据分布式计算任务分解条件建立配置文件。 该方法使用数据合并服务器导入和托管配置文件，并创建与配置文件对应的数据库表。 每个分布式节点在完成相应的分布式计算任务之后将其分布式计算结果插入到数据库表中，同时数据合并服务器将数据库中的数据合并。 可以使用适用于各种用途和各种分布式计算任务的接口标准，使得用户仅需要基于接口标准建立特定于作业的配置文件。

公开(公告)号：US20100223297A1

公开(公告)日：2010-09-02

申请号：US12158689

申请日：2008-03-28

申请人： Jianghua Li ,  Wei Wu ,  Song Chen

发明人： Jianghua Li ,  Wei Wu ,  Song Chen

IPC分类号： G06F17/30

CPC分类号： G06F9/5027 ,  G06F2209/5017

摘要： A data merging method for distributed computing uses a configuration file to guide the insertion of computational results obtained by distributed nodes into a database table, and to merge the computational results inserted in the database table. The configuration file is established according to the task splitting conditions of distributed computing. The method uses a data merging server to import and host the configuration file and create a database table corresponding to the configuration file. Each distributed node inserting its distributed computational result into the database table after completing the respective distributed computing task, while the data merging server merges the data in the database table. An interface standard applicable to various uses and various distributed computing tasks may be used such that the user only needs to establish a job-specific configuration file based on the interface standard.

摘要翻译： 用于分布式计算的数据合并方法使用配置文件来指导将由分布式节点获得的计算结果插入到数据库表中，并且合并插入到数据库表中的计算结果。 根据分布式计算任务分解条件建立配置文件。 该方法使用数据合并服务器导入和托管配置文件，并创建与配置文件对应的数据库表。 每个分布式节点在完成相应的分布式计算任务之后将其分布式计算结果插入到数据库表中，同时数据合并服务器将数据库中的数据合并。 可以使用适用于各种用途和各种分布式计算任务的接口标准，使得用户仅需要基于接口标准建立特定于作业的配置文件。