公开(公告)号：US20110045570A1

公开(公告)日：2011-02-24

申请号：US11719713

申请日：2005-11-16

申请人： Jie Lu ,  Zhijun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

发明人： Jie Lu ,  Zhijun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

IPC分类号： C12N9/52 ,  C12N9/50 ,  C07H21/04 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21

CPC分类号： C12N9/52

摘要： The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis. The protease cleaves 26 peptide bonds among 29 peptide bonds of acidic insulin B-chain.

摘要翻译： 本发明的目的在于提供一种具有高碱性，耐表面活性和钙依赖性热稳定性等特性，在高碱性洗涤剂中表现出优异性能的新型碱性蛋白酶及其氨基酸序列的编码基因。 提供了具有这样的性质的碱性蛋白酶，其活性pH范围为5至13，最佳pH为约12.6，最适温度为70℃，在65℃下不观察到加热下的活性降低 pH 10，最佳温度和热稳定性不受Ca2 +离子的影响。 具体地，例如提供了具有SEQ ID NO：3所示的构成成熟酶的氨基酸序列的氨基酸序列的碱性蛋白酶或由缺失，取代，位置反转排列，添加或插入部分 的氨基酸，或衍生自变形白菜（Alkaliphillus transvaalensis）。 蛋白酶在酸性胰岛素B链的29个肽键中切割26个肽键。

公开(公告)号：US08153413B2

公开(公告)日：2012-04-10

申请号：US11719713

申请日：2005-11-16

申请人： Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

发明人： Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

IPC分类号： C12N9/00 ,  C12N9/92 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/52

摘要： The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis. The protease cleaves 26 peptide bonds among 29 peptide bonds of acidic insulin B-chain.

摘要翻译： 本发明的目的在于提供一种具有高碱性，耐表面活性和钙依赖性热稳定性等特性，在高碱性洗涤剂中表现出优异性能的新型碱性蛋白酶及其氨基酸序列的编码基因。 提供了具有这样的性质的碱性蛋白酶，其活性pH范围为5至13，最佳pH为约12.6，最适温度为70℃，在65℃下不观察到加热下的活性降低 pH 10，最佳温度和热稳定性不受Ca2 +离子的影响。 具体地，例如提供了具有SEQ ID NO：3所示的构成成熟酶的氨基酸序列的氨基酸序列的碱性蛋白酶或由缺失，取代，位置反转排列，添加或插入部分 的氨基酸，或衍生自变形白菜（Alkaliphillus transvaalensis）。 蛋白酶在酸性胰岛素B链的29个肽键中切割26个肽键。

公开(公告)号：US07693664B2

公开(公告)日：2010-04-06

申请号：US11062833

申请日：2005-02-23

申请人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

发明人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

IPC分类号： G01N33/48

CPC分类号： G06F19/24

摘要： The invention relates to a method of judging the thermostability of a protein, comprising the steps of calculating an analytical value specific to a test protein by a principal component analysis based on the amino acid composition of the protein calculated from the data of the amino acid sequence of the protein or the nucleotide sequence of the gene and comparing the analytical value with an analytical value of a protein which is retained by a thermostable organism and corresponds to the test protein, and further relates to a program for allowing a computer to execute processing for judging the thermostability of a protein by the method, and a computer readable recording medium having recorded the program thereon.

摘要翻译： 本发明涉及一种判断蛋白质的热稳定性的方法，包括以下步骤：基于由氨基酸序列的数据计算的蛋白质的氨基酸组成，通过主成分分析计算测试蛋白质特异性的分析值 的蛋白质或该基因的核苷酸序列，并将分析值与由热稳定性生物体保留并对应于测试蛋白质的蛋白质的分析值进行比较，并且还涉及允许计算机执行处理的程序 通过该方法判断蛋白质的热稳定性，以及在其上记录有程序的计算机可读记录介质。

公开(公告)号：US20050202409A1

公开(公告)日：2005-09-15

申请号：US11062833

申请日：2005-02-23

申请人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

发明人： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

IPC分类号： C12Q1/00 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G06F19/24

摘要： The invention relates to a method of judging the thermostability of a protein, comprising the steps of calculating an analytical value specific to a test protein by a principal component analysis based on the amino acid composition of the protein calculated from the data of the amino acid sequence of the protein or the nucleotide sequence of the gene and comparing the analytical value with an analytical value of a protein which is retained by a thermostable organism and corresponds to the test protein, and further relates to a program for allowing a computer to execute processing for judging the thermostability of a protein by the method, and a computer readable recording medium having recorded the program thereon.

摘要翻译： 本发明涉及一种判断蛋白质的热稳定性的方法，包括以下步骤：基于由氨基酸序列的数据计算的蛋白质的氨基酸组成，通过主成分分析计算测试蛋白质特异性的分析值 的蛋白质或该基因的核苷酸序列，并将分析值与由热稳定性生物体保留并对应于测试蛋白质的蛋白质的分析值进行比较，并且还涉及允许计算机执行处理的程序 通过该方法判断蛋白质的热稳定性，以及在其上记录有程序的计算机可读记录介质。

公开(公告)号：US07790457B2

公开(公告)日：2010-09-07

申请号：US10591288

申请日：2005-02-28

申请人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

发明人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

IPC分类号： C12N5/00 ,  A61K9/00

CPC分类号： C12N1/22 ,  C08L1/02

摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel.The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

摘要翻译： 本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶， 为50％以上，以及其制造方法。 本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂， 凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。 提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。

公开(公告)号：US20070172938A1

公开(公告)日：2007-07-26

申请号：US10591288

申请日：2005-02-28

申请人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

发明人： Shigeru Deguchi ,  Mikiko Tsudome ,  Susumu Ito ,  Koki Horikoshi

IPC分类号： C12N1/20 ,  C08B5/00

CPC分类号： C12N1/22 ,  C08L1/02

摘要： The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel. The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

摘要翻译： 本发明涉及一种固体培养基，其中含有纤维素凝胶，特别是作为纤维素作为骨架部分并且纤维素浓度为0.01％以上的多孔纤维素凝胶结构的纤维素凝胶的纤维素凝胶， 为50％以上，以及其制造方法。 本发明的固体培养基可以通过将纤维素分散在溶剂中，特别是硫氰酸盐水溶液中，搅拌和/或加热分散体以溶解纤维素，随后冷却溶液和/或除去溶剂， 凝胶，并将渗透的营养物质加入所得的纤维素凝胶中。 提供了在不能使用常规固体培养基如琼脂培养基的广泛培养条件下可使用的固体培养基及其制备方法。

公开(公告)号：US06358700B2

公开(公告)日：2002-03-19

申请号：US09190168

申请日：1998-11-12

申请人： Abe Fumiyoshi ,  Koki Horikoshi

发明人： Abe Fumiyoshi ,  Koki Horikoshi

IPC分类号： C12Q104

CPC分类号： G01N1/30 ,  C12Q1/04 ,  G01N2001/302

摘要： A method for detecting microorganisms present in a sample comprises the steps of: applying a non-lethal hydrostatic pressure to a sample containing microorganisms; and staining the microorganisms with a fluorescent dye. The method permits a significant increase in the uptake of a fluorescent substance by applying, to a sample, a desired non-lethal hydrostatic pressure, without causing any reduction of the survival rate of microorganisms. The method also permits the elimination of such a secondary effect that the subject microorganism undergoes proliferation during staining the same with a fluorescent dye. Thus, the present invention would contribute to the determination of the correct viable count in a wide variety of technical fields.

摘要翻译： 用于检测样品中存在的微生物的方法包括以下步骤：向含有微生物的样品施加非致死性静水压力; 并用荧光染料染色微生物。 该方法允许通过向样品施加期望的非致死性静水压力而不引起微生物存活率的任何降低来显着增加荧光物质的摄取。 该方法还允许消除这样的次要作用，使得受试微生物在用荧光染料染色时经历增殖。 因此，本发明将有助于在各种各样的技术领域中确定正确的可行计数。

公开(公告)号：US5143835A

公开(公告)日：1992-09-01

申请号：US318907

申请日：1989-03-06

申请人： Naoki Nakatsugawa ,  Koki Horikoshi

发明人： Naoki Nakatsugawa ,  Koki Horikoshi

IPC分类号： C02F3/28 ,  C02F3/34 ,  C12P5/02

CPC分类号： C12R1/01 ,  C02F3/28 ,  C02F3/34 ,  C12P5/023 ,  Y02E50/343

摘要： Methanosarcina alcaliphilum which is alkalophilic methanogen having the optimum growth pH range of from about 8.1 to about 8.7. This strain exhibits excellent properties such as alkalophilicity and resistance to low temperature. This strain makes it possible to carry out methane fermentation methods at an alkaline pH and at a low temperature conditions. This strain is applied to methane fermentation in treating solid waste and waste water. Moreover, the bacterial concentration thereof in a reactor can be increased within a very short time and, therefore, the size of such a reactor can be made compact.

摘要翻译： Methanosarcina alcaliphilum，其是具有约8.1至约8.7的最佳生长pH范围的碱性产甲烷菌。 该菌株具有优良的性质，如嗜碱性和耐低温性。 该菌株可以在碱性pH和低温条件下进行甲烷发酵方法。 该菌株用于甲烷发酵处理固体废物和废水。 此外，反应器中的细菌浓度可以在非常短的时间内增加，因此可以使这种反应器的尺寸更小。

公开(公告)号：US4135977A

公开(公告)日：1979-01-23

申请号：US843263

申请日：1977-10-18

申请人： Koki Horikoshi ,  Nobuyuki Nakamura

发明人： Koki Horikoshi ,  Nobuyuki Nakamura

IPC分类号： C12N9/10 ,  C12P19/18 ,  C12D13/02

CPC分类号： C12N9/1074 ,  C12P19/18 ,  Y10S435/832

摘要： This invention provides a novel process for producing cyclodextrin by reacting cyclodextrin glycosyl transferase having an optimum pH on the alkaline side with gelatinized starch. According to this invention, cyclodextrin can be obtained in the form of a pure crystal in a high yield by reacting a glucomylase with the reaction mixture liquid formed by the above enzymatic reaction to decompose unreacted starch, concentrating the liquid, adding a small amount of cyclodextrin as a seed crystal and recovering the precipitated cyclodextrin.

公开(公告)号：US07273741B2

公开(公告)日：2007-09-25

申请号：US10252093

申请日：2002-09-23

申请人： Fumiyoshi Abe ,  Koki Horikoshi

发明人： Fumiyoshi Abe ,  Koki Horikoshi

IPC分类号： C12N9/26 ,  C12N1/14 ,  C12N1/20 ,  C08B30/04

CPC分类号： C12N9/2408 ,  C12P3/00 ,  C12R1/645

摘要： The object of the present invention is to provide a yeast which is tolerant to copper and which can incorporate copper at a high concentration, and also a method of removing or recovering copper from extracellular solution. The present invention is copper-tolerant yeast and the pectinases produced by the yeast. Particularly, the present invention is copper-tolerant yeast Cryptococcus sp. N6 strain isolated from deep-sea sediments and the pectinases produced by the yeast.

摘要翻译： 本发明的目的是提供一种对铜具有耐受性并且可以以高浓度掺入铜的酵母，以及从细胞外溶液中除去或回收铜的方法。 本发明是耐铜酵母和由酵母生产的果胶酶。 特别地，本发明是耐铜酵母隐球菌属 从深海沉积物中分离的N6菌株和由酵母产生的果胶酶。