公开(公告)号：US20140186896A1

公开(公告)日：2014-07-03

申请号：US13640471

申请日：2011-08-22

申请人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

发明人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

IPC分类号： C12P19/14 ,  C12P7/14 ,  C12P19/02

CPC分类号： C12P19/02 ,  C12N9/242 ,  C12N9/2434 ,  C12P7/10 ,  C12P7/14 ,  C12P19/14 ,  Y02E50/16 ,  Y02P20/52

摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

摘要翻译： 本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。 本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。 可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。

公开(公告)号：US09493802B2

公开(公告)日：2016-11-15

申请号：US13640471

申请日：2011-08-22

申请人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

发明人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

IPC分类号： C12P19/02 ,  C12N9/30 ,  C12N9/42 ,  C12P7/10 ,  C12P19/14 ,  C12P7/14

CPC分类号： C12P19/02 ,  C12N9/242 ,  C12N9/2434 ,  C12P7/10 ,  C12P7/14 ,  C12P19/14 ,  Y02E50/16 ,  Y02P20/52

摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

摘要翻译： 本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。 本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。 可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，β-葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。

公开(公告)号：US08298795B2

公开(公告)日：2012-10-30

申请号：US13215193

申请日：2011-08-22

申请人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

发明人： Jie Yang ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok ,  Dipnath Baidyaroy

IPC分类号： C12P19/14 ,  C12P1/00 ,  C12P7/14 ,  C12N9/42

CPC分类号： C12P19/02 ,  C12N9/242 ,  C12N9/2434 ,  C12P7/10 ,  C12P7/14 ,  C12P19/14 ,  Y02E50/16 ,  Y02P20/52

摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

摘要翻译： 本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。 本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。 可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。

公开(公告)号：US20120083019A1

公开(公告)日：2012-04-05

申请号：US13215193

申请日：2011-08-22

申请人： Dipnath Baidyaroy ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok

发明人： Dipnath Baidyaroy ,  Louis Clark ,  Onorato Campopiano ,  Kripa Rao ,  Lorand Szabo ,  Janos Torok

IPC分类号： C12P19/14 ,  C12P1/00 ,  C12P7/14 ,  C12N9/42

CPC分类号： C12P19/02 ,  C12N9/242 ,  C12N9/2434 ,  C12P7/10 ,  C12P7/14 ,  C12P19/14 ,  Y02E50/16 ,  Y02P20/52

摘要： The invention provides recombinant GH61 proteins obtained from Myceliophtora thermophila, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated from M. thermophila supernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, β-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

摘要翻译： 本发明提供了从嗜热丝霉菌（Myceliophtora thermophila）获得的重组GH61蛋白质和编码这种蛋白质的核酸。 本发明还提供从具有GH61蛋白活性的嗜热绵羊嗜热上清液分离的蛋白质级分。 可以使用这些制剂从含有纤维素的底物通过一种或多种纤维素酶如内切葡聚糖酶，葡糖苷酶或纤维二糖水解酶进行糖化的反应来提高产品的产量。 GH61蛋白和纤维素酶的组合可用于在生产乙醇中将纤维素生物质分解成可发酵糖。

公开(公告)号：US20120190076A1

公开(公告)日：2012-07-26

申请号：US13496368

申请日：2010-09-30

申请人： Louis Clark ,  Dipnath Baidyaroy ,  Lorand Szabo

发明人： Louis Clark ,  Dipnath Baidyaroy ,  Lorand Szabo

IPC分类号： C12P19/14 ,  C12N1/15 ,  C12N9/42 ,  C12N15/56 ,  C12N15/63

CPC分类号： C12P19/14 ,  C12N9/2445 ,  C12P19/02 ,  C12Y302/01021

摘要： The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

摘要翻译： 本发明涉及重组C1＆bgr-葡糖苷酶的表达。 本发明还提供了从纤维二糖生产可发酵糖的方法，该方法通过使细胞生长因子与重组宿主细胞接触，所述重组宿主细胞包含可操作地连接于异源启动子的编码C 1 - 葡萄糖苷酶的多核苷酸序列，其中所述葡糖苷酶被表达和分泌 细胞和纤维二糖由所述葡糖苷酶酶转化成葡萄糖。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。

公开(公告)号：US08900847B2

公开(公告)日：2014-12-02

申请号：US13496368

申请日：2010-09-30

申请人： Louis Clark ,  Dipnath Baidyaroy ,  Lorand Szabo

发明人： Louis Clark ,  Dipnath Baidyaroy ,  Lorand Szabo

IPC分类号： C07H21/00 ,  C12N15/00 ,  C12P21/00 ,  C12N1/21 ,  C12N5/10 ,  C12P19/14 ,  C12N9/42 ,  C12N9/24

CPC分类号： C12P19/14 ,  C12N9/2445 ,  C12P19/02 ,  C12Y302/01021

摘要： The invention relates to expression of a recombinant C1 β-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 β-glucosidase, operably linked to heterologous promoter, under conditions in which β-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said β-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

摘要翻译： 本发明涉及重组C1＆bgr-葡糖苷酶的表达。 本发明还提供了从纤维二糖生产可发酵糖的方法，该方法通过使细胞生长因子与重组宿主细胞接触，所述重组宿主细胞包含可操作地连接于异源启动子的编码C 1 - 葡萄糖苷酶的多核苷酸序列，其中所述葡糖苷酶被表达和分泌 细胞和纤维二糖由所述葡糖苷酶酶转化成葡萄糖。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。

公开(公告)号：US20090093627A1

公开(公告)日：2009-04-09

申请号：US12231438

申请日：2008-09-02

申请人： Lorand Szabo ,  Laszlo Toth ,  Nurit Perlman

发明人： Lorand Szabo ,  Laszlo Toth ,  Nurit Perlman

IPC分类号： C07D205/08 ,  C12P17/10

CPC分类号： C12P17/10

摘要： Processes of preparing an ezetimibe intermediate by microbial reduction and further converting the intermediate to ezetimibe are provided. Also provided is an ezetimibe intermediate with high diastereomeric excess.

摘要翻译： 提供了通过微生物还原制备依泽替米贝中间体并进一步将中间体转化为依泽替米贝的方法。 还提供了具有高非对映异构体过量的依泽替米贝中间体。

公开(公告)号：US07504244B2

公开(公告)日：2009-03-17

申请号：US11295142

申请日：2005-12-05

申请人： Lorand Szabo ,  Ronen Tchelet

发明人： Lorand Szabo ,  Ronen Tchelet

IPC分类号： C12P7/62 ,  C12N15/00 ,  C12N15/74 ,  C12N15/75 ,  C12N15/76 ,  C12N1/20 ,  C12N9/02 ,  C12N15/70 ,  C07H21/04

CPC分类号： C12P7/62 ,  C07K14/36 ,  C12N9/0071 ,  C12P17/06

摘要： The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to express both cytochrome P-450 and the fdxshe or fdxshe-like protein. The invention further relates to the use of such microorganisms in processes designed for production of pravastatin for use in treatment of disease such as hypercholesterolemia and hyperlipidemia.

公开(公告)号：US20090005537A1

公开(公告)日：2009-01-01

申请号：US11827200

申请日：2007-07-10

申请人： Lorand Szabo ,  Ronen Tchelet

发明人： Lorand Szabo ,  Ronen Tchelet

IPC分类号： C07K14/195

CPC分类号： C12P7/62 ,  C07K14/36 ,  C12N9/0071 ,  C12P17/06

摘要： The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to express both cytochrome P-450 and the fdxshe or fdxshe-like protein. The invention further relates to the use of such microorganisms in processes designed for production of pravastatin for use in treatment of disease such as hypercholesterolemia and hyperlipidemia.

公开(公告)号：US20060172383A1

公开(公告)日：2006-08-03

申请号：US11295142

申请日：2005-12-05

申请人： Lorand Szabo ,  Ronen Tchelet

发明人： Lorand Szabo ,  Ronen Tchelet

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12P7/62 ,  C12N9/02 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12P7/62 ,  C07K14/36 ,  C12N9/0071 ,  C12P17/06

摘要： The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to express both cytochrome P-450 and the fdxshe or fdxshe-like protein. The invention further relates to the use of such microorganisms in processes designed for production of pravastatin for use in treatment of disease such as hypercholesterolemia and hyperlipidemia.