Test system for determining an analyte in a liquid sample
    5.
    发明申请
    Test system for determining an analyte in a liquid sample 审中-公开
    用于测定液体样品中分析物的测试系统

    公开(公告)号:US20050142032A1

    公开(公告)日:2005-06-30

    申请号:US10977379

    申请日:2004-10-29

    摘要: A test system for determining an analyte in a liquid sample is provided comprising at least two compartments, wherein the detection reactions necessary to determine the analyte are carried out in a first compartment and an analytical determination of at least one substance which participates in the detection reactions and is different from the analyte, the indicator substance, takes place in a second compartment. The two compartments are separated from one another in a manner which allows the indicator substance to pass into the second compartment and at least partially prevents passage of other substances that could interfere with the analytical determination of the indicator substance in the second compartment. At least one other substance is present in an immobilized form in the second compartment which, as a capture substance, selectively enriches the indicator substance in the second compartment. The indicator substance is not a coenzyme and at the same time the capture substance is not a catalytically inactive coenzyme-binding protein.

    摘要翻译: 提供了用于确定液体样品中的分析物的测试系统,其包括至少两个隔室,其中确定分析物所需的检测反应在第一隔室中进行,并且对参与检测反应的至少一种物质进行分析测定 并且与分析物不同,指示剂物质发生在第二隔室中。 两个隔室以允许指示剂物质进入第二隔室的方式彼此分开,并且至少部分地防止可能干扰第二隔室中指示剂物质的分析确定的其它物质的通过。 至少一种其它物质以固定形式存在于第二隔室中,其作为捕获物质选择性地富集第二隔室中的指示剂物质。 指示物质不是辅酶,同时捕获物质不是催化活性的辅酶结合蛋白。

    AMINE-N-OXIDE REDOX INDICATORS FOR FLUORIMETRIC DETERMINATION OF ANALYTES
    9.
    发明申请
    AMINE-N-OXIDE REDOX INDICATORS FOR FLUORIMETRIC DETERMINATION OF ANALYTES 有权
    用于荧光测定分析仪的胺-N-氧化物氧化还原指示剂

    公开(公告)号:US20100221699A1

    公开(公告)日:2010-09-02

    申请号:US12757372

    申请日:2010-04-09

    IPC分类号: C12Q1/00 C07D271/12 G01N21/64

    摘要: A reagent for detecting an analyte by a redox reaction and a fluorimetric determination is disclosed. The reagent comprises a compound of formula (I): wherein R1 and R2 are each independently selected from R, (CH2CH2O)mR, COR, COOR, and OCOR; each R3 is independently selected from NO2, CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C1-C4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR2, COOR, CONR2, SO3R and salts thereof, and PO(OR)3 and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3. The reagent may further comprise an enzyme or a coenzyme for reducing or oxidizing an analyte.

    摘要翻译: 公开了一种用于通过氧化还原反应和荧光测定来检测分析物的试剂。 试剂包含式(I)化合物:其中R 1和R 2各自独立地选自R,(CH 2 CH 2 O)m R,COR,COOR和OCOR; 每个R 3独立地选自NO 2,CN,R,OR,OCOR,COOR,SR和卤素; R是H或C 1 -C 4烷基,其中烷基任选被一个或多个独立地选自卤素,OR,SR,NR 2,COOR,CONR 2,SO 3 R及其盐和PO(OR)3的官能团取代 及其盐; m为1〜20的整数; 并且n为1,2或3.试剂可以进一步包含用于还原或氧化分析物的酶或辅酶。