公开(公告)号：US07390389B2

公开(公告)日：2008-06-24

申请号：US10486181

申请日：2002-08-30

申请人： Joel Stephane Rossier ,  Frédéric Reymond ,  Philippe Michel

发明人： Joel Stephane Rossier ,  Frédéric Reymond ,  Philippe Michel

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44795 ,  G01N27/44773

摘要： The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.

摘要翻译： 本发明涉及一种用分离和纯化分析物溶液中的带电荷和中性化合物的装置和方法，该电泳和中性化合物通过使用多个隔室（1）以包含分析物溶液的电泳装置，化学缓冲系统（2） pH值或其与分析物溶液接触的部分中的pH梯度和由设置在所述隔室中的至少两个中的电气装置（4）施加的电场。 本发明允许差异地分离带电和中性化合物，以从化学缓冲系统（2）中提取迁移的化合物，以主要在溶液中回收纯化的化合物并将其收集在各个室（4）中，优选以各种pI 。

公开(公告)号：US07098450B2

公开(公告)日：2006-08-29

申请号：US10467973

申请日：2002-02-13

申请人： Joel Stephane Rossier ,  Frédéric Reymond ,  Hubert Hugues Girault ,  Tatiana Rohner ,  Niels Lion ,  Véronique Gobry

发明人： Joel Stephane Rossier ,  Frédéric Reymond ,  Hubert Hugues Girault ,  Tatiana Rohner ,  Niels Lion ,  Véronique Gobry

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/42 ,  G01N27/44704 ,  G01N30/6095 ,  G01N30/7266 ,  H01J49/04 ,  H01J49/165

摘要： The present invention relates to an apparatus and a method of fabricating the apparatus for dispensing a sample for subsequent analysis by mass spectrometry. The apparatus comprises a polymer substrate (1), in which a covered microchannel (3) has been etched, a wall of the microchannel comprising an electrically conductive material (2) for applying a voltage allowing the sample to be sprayed into a mass spectrometer from the open end (4) of the microchannel. The present application also discloses various applications of the apparatus.

摘要翻译： 本发明涉及一种制造用于分配样品以便随后通过质谱分析的装置的装置和方法。 该设备包括聚合物基底（1），其中已经对覆盖的微通道（3）进行蚀刻，微通道的壁包括导电材料（2），用于施加允许将样品喷射到质谱仪中的电压 微通道的开口端（4）。 本申请还公开了该设备的各种应用。

公开(公告)号：US07615354B2

公开(公告)日：2009-11-10

申请号：US10275041

申请日：2001-05-04

申请人： Michel D. Faupel ,  Hubert H. Girault ,  Frederic Reymond ,  Alexandra Ros ,  Joel Stephane Rossier

发明人： Michel D. Faupel ,  Hubert H. Girault ,  Frederic Reymond ,  Alexandra Ros ,  Joel Stephane Rossier

IPC分类号： G01N33/53

CPC分类号： G01N27/44795 ,  G01N27/44769 ,  G01N2030/0035 ,  G01N2030/285

摘要： A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

摘要翻译： 用于在分析物溶液中电荷分离和纯化带电和中性化合物的装置，方法和试剂盒。 该装置包括室（1），其至少一个壁由化学缓冲系统（4）组成。 在缓冲系统上施加电位差，导致带电和中性化合物通过将带电荷的化合物萃取到缓冲系统中而被差异地分离。 该装置还包括用于收集分离的化合物的装置，优选以不含两性电解质或不含缓冲液的溶液和用于再循环分离的级分的方法。

公开(公告)号：US20090178935A1

公开(公告)日：2009-07-16

申请号：US12296639

申请日：2007-03-29

申请人： Frederic Reymond ,  Joel Stephane Rossier ,  Patrick Morier

发明人： Frederic Reymond ,  Joel Stephane Rossier ,  Patrick Morier

IPC分类号： G01N27/26

CPC分类号： G01N27/3273

摘要： A method to optimize the amperometric detection in a microsystem consists in limiting the detection to times when the diffusion layer (18-20) of the analyte to detect remains smaller than the microchannel (7) height. The charge detected during the second part of the amperometric measurement (which corresponds to the integral of the measured current over the corresponding time period) can also be considered so as to remove the contribution of the capacitive current and, when applicable, of the current resulting from the reduction or oxidation of the analyte molecules present in a recess above the electrode at the beginning of the detection. A microfluidic amperometric sensor for performing the method comprises at least one microchannel (7) having at least one electrode (15-17), integrated in one wall of the microchannel, and having a characteristic length or radius which is smaller than half the microchannel height.

摘要翻译： 在微系统中优化电流检测的方法包括将检测限于待检测的分析物的扩散层（18-20）保持小于微通道（7）高度的时间。 在电流分析测量的第二部分期间检测到的电荷（其对应于相应时间段内的测量电流的积分）也可以被考虑，以消除电容电流的贡献，并且在适用时消除所产生的电流 从在检测开始时存在于电极上方的凹槽中的分析物分子的还原或氧化。 用于执行该方法的微流体电流计传感器包括至少一个微通道（7），其具有集成在微通道的一个壁中的至少一个电极（15-17），并且具有小于微通道高度的一半的特征长度或半径 。