公开(公告)号：US07265348B2

公开(公告)日：2007-09-04

申请号：US10534301

申请日：2003-11-07

申请人： Joël Stéphane Rossier ,  Frédéric Reymond

发明人： Joël Stéphane Rossier ,  Frédéric Reymond

IPC分类号： H01J49/00 ,  H01J49/04 ,  B01D59/44

CPC分类号： H01J49/0018 ,  H01J49/167

摘要： The present invention relates to an apparatus to dispense a sample for subsequent electrospray ionisation (ESI) mass spectrometry (MS) analysis, to a method of fabricating such apparatus and to applications of such apparatus in biological and chemical analysis. The apparatus consists of an electrically non-conductive substrate comprising at least two covered microstructures (generally microchannels) having one extremity formed at the edge of the substrate, one of said microstructures containing the sample to be dispensed into a mass spectrometer by electrospray ionisation and at least a second of said microstructure containing a fluid used as sheath liquid or sheath gas, said at least two microstructures being formed in such a manner that the sample and the sheath liquid/gas come in contact to each other and/or are mixed directly in the Taylor cone of the spray.

摘要翻译： 本发明涉及一种用于随后的电喷雾离子化（ESI）质谱（MS）分析的样品的分配装置，涉及制造这种装置的方法以及这种装置在生物和化学分析中的应用。 该装置由包括至少两个覆盖的微结构（通常为微通道）的非导电衬底组成，其具有在衬底的边缘处形成的一个末端，所述微结构之一包含通过电喷雾电离分配到质谱仪中的样品 所述至少二分之一的所述微结构包含用作鞘液或鞘气的流体，所述至少两个微结构以这样的方式形成，使得样品和鞘液/气彼此接触和/或直接混合在 泰勒锥的喷雾。

公开(公告)号：US07098450B2

公开(公告)日：2006-08-29

申请号：US10467973

申请日：2002-02-13

申请人： Joel Stephane Rossier ,  Frédéric Reymond ,  Hubert Hugues Girault ,  Tatiana Rohner ,  Niels Lion ,  Véronique Gobry

发明人： Joel Stephane Rossier ,  Frédéric Reymond ,  Hubert Hugues Girault ,  Tatiana Rohner ,  Niels Lion ,  Véronique Gobry

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/42 ,  G01N27/44704 ,  G01N30/6095 ,  G01N30/7266 ,  H01J49/04 ,  H01J49/165

摘要： The present invention relates to an apparatus and a method of fabricating the apparatus for dispensing a sample for subsequent analysis by mass spectrometry. The apparatus comprises a polymer substrate (1), in which a covered microchannel (3) has been etched, a wall of the microchannel comprising an electrically conductive material (2) for applying a voltage allowing the sample to be sprayed into a mass spectrometer from the open end (4) of the microchannel. The present application also discloses various applications of the apparatus.

摘要翻译： 本发明涉及一种制造用于分配样品以便随后通过质谱分析的装置的装置和方法。 该设备包括聚合物基底（1），其中已经对覆盖的微通道（3）进行蚀刻，微通道的壁包括导电材料（2），用于施加允许将样品喷射到质谱仪中的电压 微通道的开口端（4）。 本申请还公开了该设备的各种应用。

公开(公告)号：US07087181B2

公开(公告)日：2006-08-08

申请号：US10182577

申请日：2001-01-30

申请人： Walter Schmidt ,  Frédéric Reymond ,  Joël S. Rossier

发明人： Walter Schmidt ,  Frédéric Reymond ,  Joël S. Rossier

IPC分类号： B81C1/00

CPC分类号： H01B13/00 ,  B01F13/0059 ,  B01J2219/00828 ,  B01J2219/0086 ,  B01L3/502707 ,  B01L2200/12 ,  B29C59/14 ,  B29C2059/023 ,  B29L2009/00 ,  B81C1/00119 ,  B81C1/00206 ,  G01N27/44791 ,  G01N30/6095 ,  G01N2030/285 ,  H05K3/002 ,  H05K3/0032 ,  H05K3/0041 ,  H05K2203/0554

摘要： The technology is based on the anisotropic plasma etching of organic polymer sheets partially protected by a metallic mask. The originality of the process is to pattern the surface properties by the same physical means as the one used for the three dimensional fabrication and simultaneously to this fabrication. Surface properties means, but are not limited to hydrophobicity, hydrophilicity, conductivity, reflectability, rugosity and more precisely the chemical and/or physical state of the surface. It is also possible to generate the desired fonctionalities, for instance carboxylic acid, ester, ether, amid or imid, during the etching process. The patterning of the different properties may be achieved by two different techniques that may be used separately or simultaneously.

公开(公告)号：US07390389B2

公开(公告)日：2008-06-24

申请号：US10486181

申请日：2002-08-30

申请人： Joel Stephane Rossier ,  Frédéric Reymond ,  Philippe Michel

发明人： Joel Stephane Rossier ,  Frédéric Reymond ,  Philippe Michel

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44795 ,  G01N27/44773

摘要： The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.

摘要翻译： 本发明涉及一种用分离和纯化分析物溶液中的带电荷和中性化合物的装置和方法，该电泳和中性化合物通过使用多个隔室（1）以包含分析物溶液的电泳装置，化学缓冲系统（2） pH值或其与分析物溶液接触的部分中的pH梯度和由设置在所述隔室中的至少两个中的电气装置（4）施加的电场。 本发明允许差异地分离带电和中性化合物，以从化学缓冲系统（2）中提取迁移的化合物，以主要在溶液中回收纯化的化合物并将其收集在各个室（4）中，优选以各种pI 。