利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.035 秒)

    Methods and means for producing proteins with predetermined post-translational modifications
    1.
    发明授权
    Methods and means for producing proteins with predetermined post-translational modifications 有权
    用预定的翻译后修饰产生蛋白质的方法和方法

    公开(公告)号：US07785833B2

    公开(公告)日：2010-08-31

    申请号：US11657202

    申请日：2007-01-24

    申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianius Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianius Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    IPC分类号： C12N5/00 ,  C12N15/00 ,  G01N33/53 ,  C07K14/475

    CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

    摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules include erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties such that significant amounts of recombinant proteins can be produced that harbor “neural- or brain-type” properties.

    摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生具有“神经或脑型”特性的显着量的重组蛋白。

    Methods and means for producing proteins with predetermined post-translational modifications
    2.
    发明授权
    Methods and means for producing proteins with predetermined post-translational modifications 有权
    用预定的翻译后修饰产生蛋白质的方法和方法

    公开(公告)号：US07304031B2

    公开(公告)日：2007-12-04

    申请号：US10494140

    申请日：2002-10-29

    申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    IPC分类号： A61K38/00 ,  C07K14/505

    CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

    摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules includes erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties such that significant amounts of recombinant proteins can be produced that harbor ‘neural- or brain-type’ properties.

    摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用在很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生含有“神经或脑型”性质的显着量的重组蛋白。

    Methods and means for producing proteins with predetermined post-translational modifications
    3.
    发明授权
    Methods and means for producing proteins with predetermined post-translational modifications 失效
    用预定的翻译后修饰产生蛋白质的方法和方法

    公开(公告)号：US07696157B2

    公开(公告)日：2010-04-13

    申请号：US11888776

    申请日：2007-08-01

    申请人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    发明人： Dirk Jan Elbertus Opstelten ,  Johan Christiaan Kapteyn ,  Petrus Christianus Johannes Josephus Passier ,  Ronald Hendrik Peter Brus ,  Abraham Bout

    IPC分类号： A61K38/18 ,  C07K14/505

    CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/505 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12P21/005

    摘要： Described are methods for identifying, selecting, and obtaining mammalian cells capable of producing proteinaceous molecules having predetermined post-translational modifications, wherein the post-translational modifications are brought about by the mammalian cell in which the proteinaceous molecule is expressed. Preferably, the predetermined post-translational modifications include glycosylation. Also described are methods for obtaining and producing proteinaceous molecules, using mammalian cells obtainable by a method of the present invention. Preferably, the proteinaceous molecules include erythropoietin (EPO), since EPO's effect depends heavily on its glycosylation pattern. Mammalian cells that have been obtained on the basis of their ability to produce proteins and/or post-translational modifications that are indicative for a predetermined post-translational modification that is desired are also provided. Preferably, the mammalian cells have neural characteristics and properties, such that significant amounts of recombinant proteins can be produced that harbor “neural- or brain-type” properties.

    摘要翻译： 描述了用于鉴定，选择和获得能够产生具有预定的翻译后修饰的蛋白质分子的哺乳动物细胞的方法，其中翻译后修饰由其中表达蛋白质分子的哺乳动物细胞产生。 优选地，预定的翻译后修饰包括糖基化。 还描述了使用可通过本发明的方法获得的哺乳动物细胞获得和产生蛋白质分子的方法。 优选地，蛋白质分子包括促红细胞生成素（EPO），因为EPO的作用很大程度上取决于其糖基化模式。 还提供了基于其产生蛋白质的能力获得的哺乳动物细胞和/或指示期望的预定的翻译后修饰的翻译后修饰。 优选地，哺乳动物细胞具有神经特征和性质，使得可以产生含有“神经或脑型”性质的显着量的重组蛋白。

    Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
    6.
    发明申请
    Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby 有权
    从表达腺病毒E1A蛋白的细胞中获得具有增加的唾液酸化的重组蛋白的方法，以及由此获得的蛋白质

    公开(公告)号：US20100221774A1

    公开(公告)日：2010-09-02

    申请号：US12592384

    申请日：2009-11-23

    申请人： Dirk Jan Elbertus Opstelten

    发明人： Dirk Jan Elbertus Opstelten

    IPC分类号： C12P21/06

    CPC分类号： C07K14/505 ,  C12P21/005

    摘要： Provided are compositions comprising one or more isoforms of an erythropoietin (“EPO”) comprising glycans linked thereto, wherein the glycans have Lewis x structures and on average at least six sialic acid moieties per EPO molecule. Further provided are methods for obtaining a composition comprising one or more isoforms of EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures, the method comprising: a) providing a eukaryotic cell containing a nucleic acid sequence encoding an adenoviral E1A protein in expressible format and a nucleic acid encoding EPO in expressible format, wherein the cell further contains a nucleic acid sequence encoding a sialyltransferase, e.g., an α-2,6-sialyltransferase or an α-2,3-sialyltransferase, under control of a heterologous promoter; b) culturing the cell in a serum-free culture medium and allowing expression of EPO in the cell; c) harvesting the expressed EPO from the cell and/or from the culture medium; and d) purifying and fractionating the EPO to obtain fractions that have an increased average sialic acid content of the N-linked glycans per EPO molecule, to obtain a composition comprising one or more isoforms of an EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures.

    摘要翻译： 提供了包含一种或多种包含与其连接的聚糖的促红细胞生成素（“EPO”）的同种型的组合物，其中所述聚糖具有路易斯x结构，并且每个EPO分子平均至少六个唾液酸部分。 还提供了获得组合物的方法，所述组合物包含一种或多种包含与其连接的聚糖的EPO同种型，其中聚糖平均每EPO分子包含至少六个唾液酸和从零到两个Lewis x结构，所述方法包括：a）提供 包含编码可表达形式的腺病毒E1A蛋白的核酸序列和编码可表达形式的EPO的核酸的真核细胞，其中所述细胞还含有编码唾液酸转移酶的核酸序列，例如α-2,6-唾液酸转移酶或 在异源启动子控制下的α-2,3唾液酸转移酶; b）在无血清培养基中培养细胞并允许EPO在细胞中表达; c）从细胞和/或培养基中收获表达的EPO; 和d）纯化和分馏EPO以​​获得每EPO分子具有增加的N-连接聚糖的平均唾液酸含量增加的组分，以获得包含一种或多种包含与其连接的聚糖的EPO的同种型的组合物，其中聚糖包含 每个EPO分子平均至少六个唾液酸和从零到两个Lewis x结构。